ID   D5G3L4_CITAM            Unreviewed;       137 AA.
AC   D5G3L4;
DT   15-JUN-2010, integrated into UniProtKB/TrEMBL.
DT   15-JUN-2010, sequence version 1.
DT   27-MAR-2024, entry version 32.
DE   RecName: Full=beta-galactosidase {ECO:0000256|ARBA:ARBA00012756};
DE            EC=3.2.1.23 {ECO:0000256|ARBA:ARBA00012756};
DE   Flags: Fragment;
GN   Name=lacZ {ECO:0000313|EMBL:CAX65582.1};
OS   Citrobacter amalonaticus.
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Citrobacter.
OX   NCBI_TaxID=35703 {ECO:0000313|EMBL:CAX65582.1};
RN   [1] {ECO:0000313|EMBL:CAX65582.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=CECT 863 {ECO:0000313|EMBL:CAX65582.1};
RA   Martin M.C., Martinez N., del Rio B., Ladero V., Fernandez M.,
RA   Alvarez M.A.;
RT   "A novel real-time polymerase chain reaction-based method for the detection
RT   and quantification of lactose-fermenting Enterobacteriaceae in the dairy
RT   and other food industries.";
RL   J. Dairy Sci. 93:860-867(2010).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC         in beta-D-galactosides.; EC=3.2.1.23;
CC         Evidence={ECO:0000256|ARBA:ARBA00001412};
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DR   EMBL; FN297872; CAX65582.1; -; Genomic_DNA.
DR   AlphaFoldDB; D5G3L4; -.
DR   CAZy; GH2; Glycoside Hydrolase Family 2.
DR   GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   InterPro; IPR023232; Glyco_hydro_2_AS.
DR   InterPro; IPR006103; Glyco_hydro_2_cat.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   PANTHER; PTHR46323; BETA-GALACTOSIDASE; 1.
DR   PANTHER; PTHR46323:SF2; BETA-GALACTOSIDASE; 1.
DR   Pfam; PF02836; Glyco_hydro_2_C; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   PROSITE; PS00608; GLYCOSYL_HYDROL_F2_2; 1.
PE   4: Predicted;
KW   Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801}.
FT   DOMAIN          1..132
FT                   /note="Glycoside hydrolase family 2 catalytic"
FT                   /evidence="ECO:0000259|Pfam:PF02836"
FT   NON_TER         1
FT                   /evidence="ECO:0000313|EMBL:CAX65582.1"
FT   NON_TER         137
FT                   /evidence="ECO:0000313|EMBL:CAX65582.1"
SQ   SEQUENCE   137 AA;  15707 MW;  60DAB87603E20ACA CRC64;
     PRWYTLCDRY GLYVVDEANI ETHGMVPMNR LSDDPTWLPA MSQRVTRMVQ RDRNHPSIII
     WSLGNESGHG VNHDALYRWL KSDDPSRPVQ YEGGGADTAA TDIICPMYAR VDQDQPFPAV
     PKWSIKKWLS LPGEQRP
//