ID D5KTQ2_KLEPN Unreviewed; 277 AA.
AC D5KTQ2;
DT 15-JUN-2010, integrated into UniProtKB/TrEMBL.
DT 15-JUN-2010, sequence version 1.
DT 27-MAR-2024, entry version 81.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN ORFNames=SAMEA3538658_05391 {ECO:0000313|EMBL:SYK86374.1};
OS Klebsiella pneumoniae.
OG Plasmid PKPCAPSS {ECO:0000313|EMBL:AJF79824.1},
OG Plasmid pIT-01C22 {ECO:0000313|EMBL:CDO50774.1},
OG Plasmid pKpQIL {ECO:0000313|EMBL:ADE44027.1},
OG Plasmid pKpQIL-10 {ECO:0000313|EMBL:AHN60242.1},
OG Plasmid pKpQIL-234 {ECO:0000313|EMBL:AHN60486.1},
OG Plasmid pKpQIL-IT {ECO:0000313|EMBL:AEV55292.1},
OG Plasmid pKpQIL-LS6 {ECO:0000313|EMBL:AGO62535.1}, and
OG Plasmid pVGH151 {ECO:0000313|EMBL:AIT42022.1}.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
OX NCBI_TaxID=573 {ECO:0000313|EMBL:ADE44027.1};
RN [1] {ECO:0000313|EMBL:ADE44027.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Kpn557 {ECO:0000313|EMBL:ADE44027.1};
RC PLASMID=pKpQIL {ECO:0000313|EMBL:ADE44027.1};
RX PubMed=20696875; DOI=10.1128/AAC.00175-10;
RA Leavitt A., Chmelnitsky I., Carmeli Y., Navon-Venezia S.;
RT "Complete Nucleotide Sequence of KPC-3-Encoding Plasmid pKpQIL in the
RT Epidemic Klebsiella pneumoniae Sequence Type 258.";
RL Antimicrob. Agents Chemother. 54:4493-4496(2010).
RN [2] {ECO:0000313|EMBL:AEV55292.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ST258 {ECO:0000313|EMBL:AEV55292.1};
RC PLASMID=pKpQIL-IT {ECO:0000313|EMBL:AEV55292.1};
RX PubMed=22252815; DOI=10.1128/AAC.05308-11;
RA Garcia-Fernandez A., Villa L., Carta C., Venditti C., Giordano A.,
RA Venditti M., Mancini C., Carattoli A.;
RT "Klebsiella pneumoniae ST258 Producing KPC-3 Identified in Italy Carries
RT Novel Plasmids and OmpK36/OmpK35 Porin Variants.";
RL Antimicrob. Agents Chemother. 56:2143-2145(2012).
RN [3] {ECO:0000313|EMBL:AGO62535.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=LS6 {ECO:0000313|EMBL:AGO62535.1};
RC PLASMID=pKpQIL-LS6 {ECO:0000313|EMBL:AGO62535.1};
RX PubMed=23800906; DOI=10.1093/jac/dkt235;
RA Villa L., Capone A., Fortini D., Dolejska M., Rodriguez I., Taglietti F.,
RA De Paolis P., Petrosillo N., Carattoli A.;
RT "Reversion to susceptibility of a carbapenem-resistant clinical isolate of
RT Klebsiella pneumoniae producing KPC-3.";
RL J. Antimicrob. Chemother. 68:2482-2486(2013).
RN [4] {ECO:0000313|EMBL:AHN60242.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=BK26633 {ECO:0000313|EMBL:AHN60486.1}, and BK30799
RC {ECO:0000313|EMBL:AHN60242.1};
RC PLASMID=pKpQIL-10 {ECO:0000313|EMBL:AHN60242.1}, and pKpQIL-234
RC {ECO:0000313|EMBL:AHN60486.1};
RX PubMed=24614371; DOI=10.1128/AAC.00120-14;
RA Chen L., Chavda K.D., Melano R.G., Jacobs M.R., Koll B., Hong T.,
RA Rojtman A.D., Levi M.H., Bonomo R.A., Kreiswirth B.N.;
RT "Comparative Genomic Analysis of KPC-Encoding pKpQIL-Like Plasmids and
RT Their Distribution in New Jersey and New York Hospitals.";
RL Antimicrob. Agents Chemother. 58:2871-2877(2014).
RN [5] {ECO:0000313|EMBL:AIT42022.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=TpeVGH151 {ECO:0000313|EMBL:AIT42022.1};
RC PLASMID=pVGH151 {ECO:0000313|EMBL:AIT42022.1};
RA Chen Y.-T., Lin A.-C., Siu K.;
RT "Complete sequencing and comparative analyses of KPC-3 plasmids.";
RL Submitted (APR-2014) to the EMBL/GenBank/DDBJ databases.
RN [6] {ECO:0000313|EMBL:AJF79824.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=565 {ECO:0000313|EMBL:AJF79824.1};
RC PLASMID=PKPCAPSS {ECO:0000313|EMBL:AJF79824.1};
RA Ageevets V.A., Sopova I.V., Partina I.V., Malakhova M.V., Ilina E.N.,
RA Kostryukova E.S., Sidorenko S.V.;
RL Submitted (OCT-2014) to the EMBL/GenBank/DDBJ databases.
RN [7] {ECO:0000313|EMBL:CDO50774.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=01C22 {ECO:0000313|EMBL:CDO50774.1};
RC PLASMID=pIT-01C22 {ECO:0000313|EMBL:CDO50774.1};
RX PubMed=27334661; DOI=10.1093/jac/dkw227;
RA Di Pilato V., Papagiannitsis K., Giani T., Giakkoupi P., Riccobono E.,
RA Miriagou V., Vatopoulos A., Rossolini G.M.;
RT "Characterization of KPC-encoding plasmids from two endemic settings,
RT Greece and Italy.";
RL J. Antimicrob. Chemother. 71:2824-2830(2016).
RN [8] {ECO:0000313|EMBL:AJF79824.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=565 {ECO:0000313|EMBL:AJF79824.1};
RC PLASMID=PKPCAPSS {ECO:0000313|EMBL:AJF79824.1};
RX PubMed=27919902;
RA Ageevets V., Sopova J., Lazareva I., Malakhova M., Ilina E.,
RA Kostryukova E., Babenko V., Carattoli A., Lobzin Y., Uskov A.,
RA Sidorenko S.;
RT "Genetic Environment of the blaKPC-2 Gene in a Klebsiella pneumoniae
RT Isolate That May Have Been Imported to Russia from Southeast Asia.";
RL Antimicrob. Agents Chemother. 61:e01856-16(2017).
RN [9] {ECO:0000313|EMBL:SYK86374.1, ECO:0000313|Proteomes:UP000258808}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=EuSCAPE_IT290 {ECO:0000313|EMBL:SYK86374.1,
RC ECO:0000313|Proteomes:UP000258808};
RG Pathogen Informatics;
RL Submitted (AUG-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
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DR EMBL; GU595196; ADE44027.1; -; Genomic_DNA.
DR EMBL; JN233705; AEV55292.1; -; Genomic_DNA.
DR EMBL; JX442975; AGO62535.1; -; Genomic_DNA.
DR EMBL; KJ146687; AHN60242.1; -; Genomic_DNA.
DR EMBL; KJ146689; AHN60486.1; -; Genomic_DNA.
DR EMBL; KJ721790; AIT42022.1; -; Genomic_DNA.
DR EMBL; KP008371; AJF79824.1; -; Genomic_DNA.
DR EMBL; HG969997; CDO50774.1; -; Genomic_DNA.
DR EMBL; UKXO01000072; SYK86374.1; -; Genomic_DNA.
DR RefSeq; WP_004152751.1; NZ_WWPX01000077.1.
DR RefSeq; YP_003560457.1; NC_014016.1.
DR RefSeq; YP_006958908.1; NC_019155.1.
DR RefSeq; YP_008146423.1; NC_021655.1.
DR RefSeq; YP_009022561.1; NC_023903.1.
DR RefSeq; YP_009022668.1; NC_023904.1.
DR RefSeq; YP_009022880.1; NC_023906.1.
DR RefSeq; YP_009070167.1; NC_025166.1.
DR RefSeq; YP_009071917.1; NC_025187.1.
DR PATRIC; fig|573.1664.peg.6552; -.
DR Proteomes; UP000258808; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0006306; P:DNA methylation; IEA:InterPro.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR003356; DNA_methylase_A-5.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR42933:SF1; SITE-SPECIFIC DNA-METHYLTRANSFERASE (ADENINE-SPECIFIC); 1.
DR PANTHER; PTHR42933; SLR6095 PROTEIN; 1.
DR Pfam; PF02384; N6_Mtase; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
PE 4: Predicted;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603,
KW ECO:0000313|EMBL:AJF79824.1}; Plasmid {ECO:0000313|EMBL:ADE44027.1};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 139..238
FT /note="DNA methylase adenine-specific"
FT /evidence="ECO:0000259|Pfam:PF02384"
SQ SEQUENCE 277 AA; 31333 MW; ABD78BD00CAC4ACB CRC64;
MSQLSFADVF NMAREAADNS PVIQAGQQIA EMVPTVQRMM SEQYSRSRFI RVFKDTGRHL
GRWEVFSDFL SLAASELDMA RIRTPESMEN CRKICARYGA TDIANMQEMF CLMVCALEAK
FHDFLGAIFM ELELGDNFRG QYFTPYSVQC LMARMLMPGV RDTIRREGIA TVSDPACGAA
GMLIAYAECL LEADINPSMH MFGSCIDIDP VAADMAFIQL SLLGIAAEVV TGNTLTMQYR
RVRYTPVYYL NAFEKRLADL RRFRAMRDFL RGIQEAA
//
