GenomeNet

Database: UniProt
Entry: D6MWL8_RICCO
LinkDB: D6MWL8_RICCO
Original site: D6MWL8_RICCO 
ID   D6MWL8_RICCO            Unreviewed;       529 AA.
AC   D6MWL8;
DT   13-JUL-2010, integrated into UniProtKB/TrEMBL.
DT   13-JUL-2010, sequence version 1.
DT   27-MAR-2024, entry version 42.
DE   SubName: Full=Preproricin {ECO:0000313|EMBL:ADG29084.1};
DE   Flags: Fragment;
OS   Ricinus communis (Castor bean).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Malpighiales; Euphorbiaceae; Acalyphoideae; Acalypheae;
OC   Ricinus.
OX   NCBI_TaxID=3988 {ECO:0000313|EMBL:ADG29084.1};
RN   [1] {ECO:0000313|EMBL:ADG29084.1}
RP   NUCLEOTIDE SEQUENCE.
RX   PubMed=20211195; DOI=10.1016/j.toxicon.2010.03.001;
RA   Weeks A., Leshin J.A., Dretchen K.L., Skowronski E.W., O'Connell K.P.;
RT   "Population-level variation of the preproricin gene contradicts expectation
RT   of neutral equilibrium for generalist plant defense toxins.";
RL   Toxicon 55:1475-1483(2010).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of the N-glycosidic bond at one specific
CC         adenosine on the 28S rRNA.; EC=3.2.2.22;
CC         Evidence={ECO:0000256|ARBA:ARBA00000237};
CC   -!- SIMILARITY: In the N-terminal section; belongs to the ribosome-
CC       inactivating protein family. Type 2 RIP subfamily.
CC       {ECO:0000256|ARBA:ARBA00010414}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; GQ916875; ADG29084.1; -; Genomic_DNA.
DR   AlphaFoldDB; D6MWL8; -.
DR   GO; GO:0030598; F:rRNA N-glycosylase activity; IEA:UniProtKB-EC.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0017148; P:negative regulation of translation; IEA:UniProtKB-KW.
DR   CDD; cd00161; RICIN; 2.
DR   Gene3D; 2.80.10.50; -; 2.
DR   Gene3D; 3.40.420.10; Ricin (A subunit), domain 1; 1.
DR   Gene3D; 4.10.470.10; Ricin (A Subunit), domain 2; 1.
DR   InterPro; IPR036041; Ribosome-inact_prot_sf.
DR   InterPro; IPR017989; Ribosome_inactivat_1/2.
DR   InterPro; IPR001574; Ribosome_inactivat_prot.
DR   InterPro; IPR017988; Ribosome_inactivat_prot_CS.
DR   InterPro; IPR016138; Ribosome_inactivat_prot_sub1.
DR   InterPro; IPR016139; Ribosome_inactivat_prot_sub2.
DR   InterPro; IPR035992; Ricin_B-like_lectins.
DR   InterPro; IPR000772; Ricin_B_lectin.
DR   PANTHER; PTHR33453; -; 1.
DR   PANTHER; PTHR33453:SF34; DUF6598 DOMAIN-CONTAINING PROTEIN; 1.
DR   Pfam; PF00652; Ricin_B_lectin; 2.
DR   Pfam; PF00161; RIP; 1.
DR   PRINTS; PR00396; SHIGARICIN.
DR   SMART; SM00458; RICIN; 2.
DR   SUPFAM; SSF56371; Ribosome inactivating proteins (RIP); 1.
DR   SUPFAM; SSF50370; Ricin B-like lectins; 2.
DR   PROSITE; PS50231; RICIN_B_LECTIN; 2.
DR   PROSITE; PS00275; SHIGA_RICIN; 1.
PE   3: Inferred from homology;
KW   Disulfide bond {ECO:0000256|ARBA:ARBA00023157};
KW   Glycoprotein {ECO:0000256|ARBA:ARBA00023180};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW   Protein synthesis inhibitor {ECO:0000256|ARBA:ARBA00023193};
KW   Toxin {ECO:0000256|ARBA:ARBA00022656}.
FT   DOMAIN          301..427
FT                   /note="Ricin B lectin"
FT                   /evidence="ECO:0000259|SMART:SM00458"
FT   DOMAIN          431..529
FT                   /note="Ricin B lectin"
FT                   /evidence="ECO:0000259|SMART:SM00458"
FT   NON_TER         1
FT                   /evidence="ECO:0000313|EMBL:ADG29084.1"
FT   NON_TER         529
FT                   /evidence="ECO:0000313|EMBL:ADG29084.1"
SQ   SEQUENCE   529 AA;  58702 MW;  92865CC04C96BC0A CRC64;
     GSTSGWSFTL EDNNIFPKQY PIINFTTAGA TVQSYTNFIR AVRGRLTTGA DVRHEIPVLP
     NRVGLPINQR FILVELSNHA ELSVTLALDV TNAYVVGYRA GNSAYFFHPD NQEDAEAITH
     LFTDVQNRYT FAFGGNYDRL EQLAGNLREN IELGNGPLEE AISALYYYST GGTQLPTLAR
     SFIICIQMIS EAARFQYIEG EMRTRIRYNR RSAPDPSVIT LENSWGRLST AIQESNQGAF
     ASPIQLQRRN GSKFSVYDVS ILIPIIALMV YRCAPPPSSQ FSLLIRPVVP NFNADVCMDP
     EPIVRIVGRN GLCVDVRDGR FHNGNAIQLW PCKSNTDANQ LWTLKRDNTI RSNGKCLTTY
     GYSPGVYVMI YDCNTAATDA TRWQIWDNGT IINPRSSLVL AATSGNSGTT LTVQTNIYAV
     SQGWLPTNNT QPFVTTIVGL YGLCLQANSG QVWIEDCSSE KAEQQWALYA DGSIRPQQNR
     DNCLTSDSNI RETVVKILSG GPASSGQRWM FKNDGTILNL YSGLVLDVR
//
DBGET integrated database retrieval system