ID E1F763_GIAIA Unreviewed; 2632 AA.
AC E1F763;
DT 30-NOV-2010, integrated into UniProtKB/TrEMBL.
DT 30-NOV-2010, sequence version 1.
DT 24-JAN-2024, entry version 51.
DE RecName: Full=HECT-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012485};
DE EC=2.3.2.26 {ECO:0000256|ARBA:ARBA00012485};
GN ORFNames=GLP15_2224 {ECO:0000313|EMBL:EFO61735.1};
OS Giardia intestinalis (strain P15) (Giardia lamblia).
OC Eukaryota; Metamonada; Diplomonadida; Hexamitidae; Giardiinae; Giardia.
OX NCBI_TaxID=658858 {ECO:0000313|EMBL:EFO61735.1, ECO:0000313|Proteomes:UP000008974};
RN [1] {ECO:0000313|EMBL:EFO61735.1, ECO:0000313|Proteomes:UP000008974}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=P15 {ECO:0000313|EMBL:EFO61735.1,
RC ECO:0000313|Proteomes:UP000008974};
RX PubMed=20929575; DOI=10.1186/1471-2164-11-543;
RA Jerlstrom-Hultqvist J., Franzen O., Ankarklev J., Xu F., Nohynkova E.,
RA Andersson J.O., Svard S.G., Andersson B.;
RT "Genome analysis and comparative genomics of a Giardia intestinalis
RT assemblage E isolate.";
RL BMC Genomics 11:543-543(2010).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.26; Evidence={ECO:0000256|ARBA:ARBA00000885};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EFO61735.1}.
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DR EMBL; ACVC01000211; EFO61735.1; -; Genomic_DNA.
DR STRING; 658858.E1F763; -.
DR EnsemblProtists; EFO61735; EFO61735; GLP15_2224.
DR VEuPathDB; GiardiaDB:GLP15_2224; -.
DR OMA; ICSIYLI; -.
DR OrthoDB; 5478214at2759; -.
DR Proteomes; UP000008974; Unassembled WGS sequence.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR Gene3D; 3.30.2410.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.90.1750.10; Hect, E3 ligase catalytic domains; 1.
DR InterPro; IPR000569; HECT_dom.
DR InterPro; IPR035983; Hect_E3_ubiquitin_ligase.
DR PANTHER; PTHR11254:SF67; E3 UBIQUITIN-PROTEIN LIGASE HUWE1; 1.
DR PANTHER; PTHR11254; HECT DOMAIN UBIQUITIN-PROTEIN LIGASE; 1.
DR Pfam; PF00632; HECT; 1.
DR SMART; SM00119; HECTc; 1.
DR SUPFAM; SSF56204; Hect, E3 ligase catalytic domain; 1.
DR PROSITE; PS50237; HECT; 1.
PE 4: Predicted;
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786,
KW ECO:0000256|PROSITE-ProRule:PRU00104}.
FT DOMAIN 2290..2632
FT /note="HECT"
FT /evidence="ECO:0000259|PROSITE:PS50237"
FT REGION 302..328
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 548..586
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 616..649
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1708..1737
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1934..1957
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 555..573
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 628..644
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 2600
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00104"
SQ SEQUENCE 2632 AA; 292790 MW; CF9F40725E3309B1 CRC64;
MEGEYDSDII PYDPPQPKSA EDFLFNPNPS RFRFTMPPRW QDLDPPLLVT ETCNVELTRG
RETILSIGRP RTRQRRLRRT LAWLRDHTSC LSTAYNCLYD SGIFVSVSDH VHNTMEVSTI
ASLSLISDAQ TNPPSSRPSS AGSVEVQTFL QNCLNNFTDP AALKHHTQML RENNNLFPSE
LVTASVIGKV RDCLAELAAM LSNSYSVPGV LNISQKQFLT ELLRRTNKFF LSNSRRYESA
LSFVCALWPL WVYLSQVFDK AICDVVSINL NTIAKFYNIN VSKRVGEYIQ AMQELDSARQ
NLNKSADDPE ESTGKSNESK APSTPSIDNV ISSLPSHSLL RRFCDTTFKN PFLQATSCKL
ICQLFTVCND LVPFHPDLRL LIYGSVTFES LQSLPLSVES IKIARYTSSK VFLLQAQRVY
RKYGDEIAAN ADLDDLTLLL HDYYFHVIHS MTEDGVEELK GTPCDVSHSG YVHHSSLNTV
SRNNEPEETI TDMFYKRGKE VDRAVLNAIN LLKERAREDA VSPVFNGDDG SSDEDDTAIA
AELARLMAAP PSESDSDIAE STTTATTATD VSEEESGHNN DPIDELDAAL PNSLCTAVDV
PDNATSATAE PVLEATVTQL DDTPNEQDPT PRLSEDRLNE QAEEGPTEDV LQSDVYDIRQ
MRVISEDYVS NWLSQNFLED SPSMSLETRM RYMCMFISSV SLAVSEVDSG AINTELQQAQ
SFSEQEEYLF SQAIEKLATL FVEAISLDAP LNNVSITIVH EFLCIFLYCT GLGFVGEFEC
INLDTFLQIL QHIDLYSETI RSVNRYIKSM SLLQNEDIRG SLVSYDKKIF NFLLKKFSVL
DDTYYLNAGN PMPVRDIKVT HLQLRVQNAD ALSTTVRDLQ RLVERQCNMT TCEFISSCLI
EHQCLNCILI DLIIKSQKDF AAFLIANPDQ LLQILHVCKN NLLDNVPAKE LATENIDVTL
RNRLSYFLLM LFILSLLDTD TIVHSMQPLL SLATNAEEIC VDGNAAEDNT VGAEKQCATS
DPTRAMSKFT IDNIVTLICA YLQSICKYSQ QYSTDRSITY ALNTLNKQTR GSGKAIKIPF
ELFRNKVDGS VYFVEGTNVA KLLTIITEPC YAGLFEKSYA LIPKLKVRSD AGGIARRLLT
TGHLPLAGMR TLNTDLYDRI KLMTGRERTE DLRYVDKAIP RILLASLFLD RVAKSRIPIS
ASPLESLLMT LEYLVHKKLT THLADPPDGD SVLAQGLFCF PAGIKPKVSS FIVSLFALSK
HPISMTLSYA LFHWLELPAT ELALFYNALE KLPNFGFINL PTERIAEALA AKDDPESLDI
VDPHSAEQAP VSLLAQQGLP GILVATQAQA ETSSLGLASS TSTDLLDQSF IHPPIDKLLS
SMGTQSTCAF QYQAQVALEE YTSLAQLFYR SFTHVIVTLL FALGNWKCYP LGQFLYLPAE
RISRLQGKIF SIPDRLAMLN SLSVILGEIE AIVAFIPFIQ QTLPIVTLLS GCLAHIDEAI
FLGNEPIIRT LLRVLLPGEL YFSLMRPPSA QPGQMHSLTS SLATKPLSAG GSILPARPLS
SSLTTKPGYF MTASTTQSYR GLSVSLKTKQ MVLACLRTTL MNAIQLCALS TLPQTYPFYN
IYTNELLQFS QLEICSIYLI GWLRLTQNLT LCYDGALVAQ LITILSYFPH LKYDIQRSIP
EESSELEHAA SFNAFGSLTS NATPLMHTNS PALRPSSRAS TASKATLRGT SVGTSSLNSR
SIPGLFSPNF NVVSTPFIAL LFPAFTFDGA VSNFVGWIIY HFLLGYVKLG GLEGMLHQIH
ISEESVPLAG LPVHANLDSG SKKYIESLTN YFSTQMESVS ACKNICSLVS AFTFVLQYDM
LHLVGSSVQL GHKLSAHVLQ KMLMALPETV CSTGECMFDD PNSTSRMAKI LVPLSAPLGA
FSTAGCFAID LPGKSTTKTD PDEADTTEEQ TEETKEETEK QAFGTKMVDV YIAVSKTAGS
LSETHFSYLS AESGLADVWT SYQVFLTSEY NEYFFLQSHA IVDPACLCGD HLGIFFALCS
YHIWFRMLLD PLFRRSTLIM SVDNQTDPYY ALYSEVQHAH RYYCLEANNL SSVWFGADLI
AITRILRHPN AEIDEADIAH RKQLCLLKRA CIYIAELKEA LHVKSLAEGS YSHLIAQQNA
MKSVEEHTIL SLVLPNSSID DHTITQILME DLAAQSKQEY NSVVFLGALS DIYLNGAALF
MLSFDDKLFM FRHLMRLSSN CYLPEQTMSE TMVEATKYVP AQSTTLEISV RRDAPLQSLI
EYLDQNVAGS QAALQKPWRV AFLDENALDL HGPRQEYMNL VTSDLMSHCQ AFSRKNYLPK
VCKASAAPLR RQSGYLEYII LAYLISRSIT EFLSLSFDVP TTLLRRILGL PFKAQDIMLS
SPELYYGNLI ALLNVDKYRF EDCVGLDITD VPFDDTIDAE HSTIKYSGNT MDHVNMMVAE
IISRRSVPIN VVAKLVQRSL LSVDLRIFTP VELGLVISGI NELSSAQLIS LFKWIHPPPA
VSSGLVSQAT ELAFSTVLNE LTCEERSEFL CFITGSSRVP PIGFEPELKV QRLAADGETP
DTKPEDIDPK KLRLPSASTC FHSLKLPPYC NPADLKRVLK ISISEGKTFE FA
//
