ID E1RGI7_METP4 Unreviewed; 240 AA.
AC E1RGI7;
DT 30-NOV-2010, integrated into UniProtKB/TrEMBL.
DT 30-NOV-2010, sequence version 1.
DT 27-MAR-2024, entry version 60.
DE RecName: Full=Putative thymidylate synthase {ECO:0000256|HAMAP-Rule:MF_01686};
DE Short=TS {ECO:0000256|HAMAP-Rule:MF_01686};
DE Short=TSase {ECO:0000256|HAMAP-Rule:MF_01686};
DE EC=2.1.1.- {ECO:0000256|HAMAP-Rule:MF_01686};
GN Name=thyA {ECO:0000256|HAMAP-Rule:MF_01686};
GN OrderedLocusNames=Mpet_0424 {ECO:0000313|EMBL:ADN35198.1};
OS Methanolacinia petrolearia (strain DSM 11571 / OCM 486 / SEBR 4847)
OS (Methanoplanus petrolearius).
OC Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC Methanomicrobiales; Methanomicrobiaceae; Methanolacinia.
OX NCBI_TaxID=679926 {ECO:0000313|EMBL:ADN35198.1, ECO:0000313|Proteomes:UP000006565};
RN [1] {ECO:0000313|EMBL:ADN35198.1, ECO:0000313|Proteomes:UP000006565}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 11571 / OCM 486 / SEBR 4847
RC {ECO:0000313|Proteomes:UP000006565};
RX PubMed=21304750;
RA Brambilla E., Djao O.D., Daligault H., Lapidus A., Lucas S., Hammon N.,
RA Nolan M., Tice H., Cheng J.F., Han C., Tapia R., Goodwin L., Pitluck S.,
RA Liolios K., Ivanova N., Mavromatis K., Mikhailova N., Pati A., Chen A.,
RA Palaniappan K., Land M., Hauser L., Chang Y.J., Jeffries C.D., Rohde M.,
RA Spring S., Sikorski J., Goker M., Woyke T., Bristow J., Eisen J.A.,
RA Markowitz V., Hugenholtz P., Kyrpides N.C., Klenk H.P.;
RT "Complete genome sequence of Methanoplanus petrolearius type strain (SEBR
RT 4847).";
RL Stand. Genomic Sci. 3:203-211(2010).
CC -!- FUNCTION: May catalyze the biosynthesis of dTMP using an unknown
CC cosubstrate. {ECO:0000256|HAMAP-Rule:MF_01686}.
CC -!- PATHWAY: Pyrimidine metabolism; dTTP biosynthesis. {ECO:0000256|HAMAP-
CC Rule:MF_01686}.
CC -!- SUBUNIT: Monomer. {ECO:0000256|HAMAP-Rule:MF_01686}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_01686}.
CC -!- SIMILARITY: Belongs to the thymidylate synthase family. Archaeal-type
CC ThyA subfamily. {ECO:0000256|HAMAP-Rule:MF_01686}.
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DR EMBL; CP002117; ADN35198.1; -; Genomic_DNA.
DR RefSeq; WP_013328376.1; NC_014507.1.
DR AlphaFoldDB; E1RGI7; -.
DR STRING; 679926.Mpet_0424; -.
DR GeneID; 9742871; -.
DR KEGG; mpi:Mpet_0424; -.
DR eggNOG; arCOG03214; Archaea.
DR HOGENOM; CLU_084975_0_0_2; -.
DR OrthoDB; 50118at2157; -.
DR UniPathway; UPA00575; -.
DR Proteomes; UP000006565; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004799; F:thymidylate synthase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006231; P:dTMP biosynthetic process; IEA:UniProtKB-UniRule.
DR GO; GO:0006235; P:dTTP biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR CDD; cd00351; TS_Pyrimidine_HMase; 1.
DR Gene3D; 3.30.572.10; Thymidylate synthase/dCMP hydroxymethylase domain; 1.
DR HAMAP; MF_01686; Thymidy_synth_arch; 1.
DR InterPro; IPR045097; Thymidate_synth/dCMP_Mease.
DR InterPro; IPR023451; Thymidate_synth/dCMP_Mease_dom.
DR InterPro; IPR036926; Thymidate_synth/dCMP_Mease_sf.
DR InterPro; IPR014620; Thymidylate_synthase_arc.
DR NCBIfam; TIGR03283; thy_syn_methano; 1.
DR PANTHER; PTHR11548; THYMIDYLATE SYNTHASE 1; 1.
DR PANTHER; PTHR11548:SF1; THYMIDYLATE SYNTHASE-RELATED; 1.
DR Pfam; PF00303; Thymidylat_synt; 1.
DR PIRSF; PIRSF036752; TSase_MJ051; 1.
DR SUPFAM; SSF55831; Thymidylate synthase/dCMP hydroxymethylase; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|HAMAP-Rule:MF_01686};
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|HAMAP-
KW Rule:MF_01686}; Nucleotide biosynthesis {ECO:0000256|HAMAP-Rule:MF_01686};
KW Reference proteome {ECO:0000313|Proteomes:UP000006565};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|HAMAP-
KW Rule:MF_01686}.
FT DOMAIN 82..211
FT /note="Thymidylate synthase/dCMP hydroxymethylase"
FT /evidence="ECO:0000259|Pfam:PF00303"
FT ACT_SITE 145
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01686"
SQ SEQUENCE 240 AA; 27738 MW; 66021A1663DF9317 CRC64;
MFNVRAFSLG KAHEEVIKTI LKHGKYLVTE DGEKTIELPE PLNIHVKNPF EDYMISPFNM
FGEGAMRQYV NDLLNGTENE FVYTYHDRLF DYPVGDSEFR GDGDGKGIDQ VEYLIEKLKA
EPNSRRAEAI TWFPPKDDQS DNPPCLQRIQ CFVRDGRLNM HVDFRSNDML SALGANMYAL
VHLQKMIADR LKADVGWYSH TSVSAHMYHE RDHDELMKYV KGLGLEADLK HYESSKLIKD
//
