ID E2AYG2_CAMFO Unreviewed; 537 AA.
AC E2AYG2;
DT 30-NOV-2010, integrated into UniProtKB/TrEMBL.
DT 30-NOV-2010, sequence version 1.
DT 27-MAR-2024, entry version 46.
DE RecName: Full=Luciferin 4-monooxygenase {ECO:0000256|ARBA:ARBA00019043};
DE EC=1.13.12.7 {ECO:0000256|ARBA:ARBA00012532};
GN ORFNames=EAG_11733 {ECO:0000313|EMBL:EFN61497.1};
OS Camponotus floridanus (Florida carpenter ant).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Hymenoptera; Apocrita; Aculeata; Formicoidea;
OC Formicidae; Formicinae; Camponotus.
OX NCBI_TaxID=104421 {ECO:0000313|Proteomes:UP000000311};
RN [1] {ECO:0000313|EMBL:EFN61497.1, ECO:0000313|Proteomes:UP000000311}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C129 {ECO:0000313|Proteomes:UP000000311};
RX PubMed=20798317; DOI=10.1126/science.1192428;
RA Bonasio R., Zhang G., Ye C., Mutti N.S., Fang X., Qin N., Donahue G.,
RA Yang P., Li Q., Li C., Zhang P., Huang Z., Berger S.L., Reinberg D.,
RA Wang J., Liebig J.;
RT "Genomic comparison of the ants Camponotus floridanus and Harpegnathos
RT saltator.";
RL Science 329:1068-1071(2010).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + firefly D-luciferin + O2 = AMP + CO2 + diphosphate +
CC firefly oxyluciferin + hnu; Xref=Rhea:RHEA:10732, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:16526, ChEBI:CHEBI:16792, ChEBI:CHEBI:30212,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:58038,
CC ChEBI:CHEBI:456215; EC=1.13.12.7;
CC Evidence={ECO:0000256|ARBA:ARBA00001278};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- SUBCELLULAR LOCATION: Peroxisome {ECO:0000256|ARBA:ARBA00004275}.
CC -!- SIMILARITY: Belongs to the ATP-dependent AMP-binding enzyme family.
CC {ECO:0000256|ARBA:ARBA00006432}.
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DR EMBL; GL443910; EFN61497.1; -; Genomic_DNA.
DR RefSeq; XP_011266235.1; XM_011267933.2.
DR RefSeq; XP_011266236.1; XM_011267934.2.
DR AlphaFoldDB; E2AYG2; -.
DR STRING; 104421.E2AYG2; -.
DR EnsemblMetazoa; XM_011267933.3; XP_011266235.1; LOC105257365.
DR EnsemblMetazoa; XM_011267934.3; XP_011266236.1; LOC105257365.
DR GeneID; 105257365; -.
DR KEGG; cfo:105257365; -.
DR InParanoid; E2AYG2; -.
DR OMA; SISCNQA; -.
DR OrthoDB; 1404660at2759; -.
DR Proteomes; UP000000311; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0005777; C:peroxisome; IEA:UniProtKB-SubCell.
DR GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR GO; GO:0008218; P:bioluminescence; IEA:UniProtKB-KW.
DR CDD; cd05911; Firefly_Luc_like; 1.
DR Gene3D; 3.30.300.30; -; 1.
DR Gene3D; 3.40.50.980; -; 2.
DR InterPro; IPR025110; AMP-bd_C.
DR InterPro; IPR045851; AMP-bd_C_sf.
DR InterPro; IPR020845; AMP-binding_CS.
DR InterPro; IPR000873; AMP-dep_Synth/Lig_com.
DR PANTHER; PTHR24096:SF353; GH16244P-RELATED; 1.
DR PANTHER; PTHR24096; LONG-CHAIN-FATTY-ACID--COA LIGASE; 1.
DR Pfam; PF00501; AMP-binding; 1.
DR Pfam; PF13193; AMP-binding_C; 1.
DR SUPFAM; SSF56801; Acetyl-CoA synthetase-like; 1.
DR PROSITE; PS00455; AMP_BINDING; 1.
PE 3: Inferred from homology;
KW Luminescence {ECO:0000256|ARBA:ARBA00023223};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Monooxygenase {ECO:0000256|ARBA:ARBA00023033, ECO:0000313|EMBL:EFN61497.1};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023033,
KW ECO:0000313|EMBL:EFN61497.1}; Peroxisome {ECO:0000256|ARBA:ARBA00023140};
KW Photoprotein {ECO:0000256|ARBA:ARBA00023262};
KW Reference proteome {ECO:0000313|Proteomes:UP000000311};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 228..259
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 26..397
FT /note="AMP-dependent synthetase/ligase"
FT /evidence="ECO:0000259|Pfam:PF00501"
FT DOMAIN 448..524
FT /note="AMP-binding enzyme C-terminal"
FT /evidence="ECO:0000259|Pfam:PF13193"
SQ SEQUENCE 537 AA; 60302 MW; 79E77D4747259598 CRC64;
MGKIFERRLE RPSPTISVGQ AILEKLRENG DEVLLIDGHN GNQMTNKGLL EKSVKFANFL
QKNGIKIGDR IAIASENRID WLIPACATFY IGAILASYNP LYTEYEFQYL LNIAKPRIIL
VSQRTERLIA KIVPNLSWKI TLIELDDQPF ATDIRTLKDI LNNEEAVDFL GYKAVDIGDN
SKHPLVILCS SGTTGLPKGV TLSHKNLLAF ISKITQPEYL DIRHRDRILL FLPFFHGYAT
GMLLACMYTG SIIVMMSAFE QELFLDLIQK YKITHLPLVP PILIFLAKHP LVDKYDFRSV
RELVCGAAPL AMDVAAAVKS RIGIKYIRNG YGMTELSVVS NLCGREDEDE SFENAATGVS
LPGFLSKVVD LETQETLDVG QVGEICYMGE QLMMGYWNNS EATRQTIDHD GWLHTGDVGY
FDDKGNLRVV NRIKELIKYK GYQVSPTEIE TVLLSHPAVK DTAVIGRPDE RSGEIPMAFI
VRQPGTTITV QDLQDFVKQK LSPQKWLRGG VQFIDAIPKN PSGKILRREL QAMIPKL
//
