ID E2ZBG2_9FIRM Unreviewed; 1220 AA.
AC E2ZBG2;
DT 11-JAN-2011, integrated into UniProtKB/TrEMBL.
DT 11-JAN-2011, sequence version 1.
DT 27-MAR-2024, entry version 68.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN Name=addA {ECO:0000313|EMBL:EFQ04359.1};
GN ORFNames=HMPREF9429_00789 {ECO:0000313|EMBL:EFQ04359.1};
OS Megasphaera micronuciformis F0359.
OC Bacteria; Bacillota; Negativicutes; Veillonellales; Veillonellaceae;
OC Megasphaera.
OX NCBI_TaxID=706434 {ECO:0000313|EMBL:EFQ04359.1, ECO:0000313|Proteomes:UP000003195};
RN [1] {ECO:0000313|EMBL:EFQ04359.1, ECO:0000313|Proteomes:UP000003195}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=F0359 {ECO:0000313|EMBL:EFQ04359.1,
RC ECO:0000313|Proteomes:UP000003195};
RA Weinstock G., Sodergren E., Clifton S., Fulton L., Fulton B., Courtney L.,
RA Fronick C., Harrison M., Strong C., Farmer C., Delahaunty K., Markovic C.,
RA Hall O., Minx P., Tomlinson C., Mitreva M., Hou S., Chen J., Wollam A.,
RA Pepin K.H., Johnson M., Bhonagiri V., Zhang X., Suruliraj S., Warren W.,
RA Chinwalla A., Mardis E.R., Wilson R.K.;
RL Submitted (AUG-2010) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EFQ04359.1}.
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DR EMBL; AECS01000035; EFQ04359.1; -; Genomic_DNA.
DR AlphaFoldDB; E2ZBG2; -.
DR STRING; 706434.HMPREF9429_00789; -.
DR eggNOG; COG1074; Bacteria.
DR HOGENOM; CLU_001114_3_1_9; -.
DR Proteomes; UP000003195; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0004527; F:exonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0006302; P:double-strand break repair; IEA:InterPro.
DR Gene3D; 3.90.320.10; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 4.
DR InterPro; IPR014152; AddA.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR011604; PDDEXK-like_dom_sf.
DR InterPro; IPR038726; PDDEXK_AddAB-type.
DR InterPro; IPR011335; Restrct_endonuc-II-like.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR NCBIfam; TIGR02785; addA_Gpos; 1.
DR PANTHER; PTHR11070:SF48; ATP-DEPENDENT HELICASE_NUCLEASE SUBUNIT A; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF12705; PDDEXK_1; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF52980; Restriction endonuclease-like; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Exonuclease {ECO:0000256|ARBA:ARBA00022839};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000003195}.
FT DOMAIN 5..481
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 517..795
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT BINDING 26..33
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 1220 AA; 138858 MW; 1909BF3D98A130B2 CRC64;
MVDKMAWTEA QEKAITSRNQ NLLLSAAAGS GKTAVLVERI IRRLIDVDNP TDITEIMVVT
FTKAAAGEMR ERIGGALLKA AEDETLSAAV RESARRQAAL LPSARISTFH AFCQYVLRTR
FYTIDLDPQF TIAGTEELEL LKQSVWDDVA LSYYEDEEKV RTMTALTDVF GNDRGDAGLT
EIVMKAYEYI RSQPWPLQWL EEATAAYEKV DSSTDLGSLP WASPILANIR SALSRCADEY
DESLKILEYV EELNGQYERF KEERNRFKAA SEIKSWSRME DFFSDFDFGR LKSMRNLVAD
VADAWNDCKA HRDKAKDTVK KIRDTYFTKT SKQWVDDLIQ AGHTMSALSG LIRDFYKAYN
AAKREKNWLD FGDLEHYCLQ ILTVHDEQTG EVLPSAAAKE MRSTFKEILI DEYQDTNGVQ
ELITTLVSDG TNRFMVGDIK QSIYRFRLAD PTLFLEKYMT FERRAEAVNR CIVLSMTFRS
APAVIEAVND IFSYAMTEAA TGMDYGDNEK LYVGRTDDEP GVAELHILDE VTTYESLETD
GGEESDDDDD SAEESSFIRQ CRFVAARIGE LKAADPSLKY KDIVVLLRSV SRKAEDLLTV
LQDAGIPAYA EQKGGYFNVT EVRLMTALLA CIDNPCRDIE LAAVLRSPIV GINEATLAEI
RMYRDGYLVD ALEAYTAGLH DGVYKERLSE FLFRLDAWRT YSRRAGVAEL LRRIYEKTSY
IDYVAAMKNG DLRRANLEAL YERAGQYERA GFRGLFRFLK FIERMKKKGL DLAPARVVGE
EEDVVRIMTI HKSKGLEFPV VFLVNTEKTF NKQDEQEPVL YHKELGIGIR AFDAKWRMTW
PTLAYNGIKE RTVWENRAEE ERLLYVAMTR AKNRLILVGT VKSRGSKNTD CLYDRLARFR
NDVHAEAGTS YLDWILPPLL RKLPDVTDLT ASDENSEFVH AGVWQVTVHT EPVDAGKAMA
DVLRPEEAHL EAVRADMPTG TAIPAWLDEA LSFTYPFEAA THTVAKLSVS EIKRRLAEAT
EEEDLLQEGE HLFSTGVEDD ETDLTRPPWL CEKASQSPTF AGTVFHKAVQ LLDFADLTKE
NIVEQVEGWK NSGRFTSEEL SRLRMNDLYH FAESHLAARI QASPDVRREY GFTFLADSDK
VSRVEGERIL VQGVVDCLFV EGDEWIIVDY KTDRLNTADE FKKRYAVQLN LYKLAVEQIS
QRKVKEMIIY SSRLGKEIFI
//
