ID E3RH37_PYRTT Unreviewed; 1569 AA.
AC E3RH37;
DT 11-JAN-2011, integrated into UniProtKB/TrEMBL.
DT 11-JAN-2011, sequence version 1.
DT 24-JAN-2024, entry version 55.
DE RecName: Full=Glycogen debranching enzyme {ECO:0000256|ARBA:ARBA00020723};
DE EC=2.4.1.25 {ECO:0000256|ARBA:ARBA00012560};
DE EC=3.2.1.33 {ECO:0000256|ARBA:ARBA00012778};
DE AltName: Full=Glycogen debrancher {ECO:0000256|ARBA:ARBA00031477};
GN ORFNames=PTT_07191 {ECO:0000313|EMBL:EFQ94963.1};
OS Pyrenophora teres f. teres (strain 0-1) (Barley net blotch fungus)
OS (Drechslera teres f. teres).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Dothideomycetes;
OC Pleosporomycetidae; Pleosporales; Pleosporineae; Pleosporaceae;
OC Pyrenophora.
OX NCBI_TaxID=861557 {ECO:0000313|Proteomes:UP000001067};
RN [1] {ECO:0000313|EMBL:EFQ94963.1, ECO:0000313|Proteomes:UP000001067}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=0-1 {ECO:0000313|EMBL:EFQ94963.1,
RC ECO:0000313|Proteomes:UP000001067};
RX PubMed=21067574; DOI=10.1186/gb-2010-11-11-r109;
RA Ellwood S.R., Liu Z., Syme R.A., Lai Z., Hane J.K., Keiper F., Moffat C.S.,
RA Oliver R.P., Friesen T.L.;
RT "A first genome assembly of the barley fungal pathogen Pyrenophora teres f.
RT teres.";
RL Genome Biol. 11:R109.1-R109.14(2010).
CC -!- FUNCTION: Multifunctional enzyme acting as 1,4-alpha-D-glucan:1,4-
CC alpha-D-glucan 4-alpha-D-glycosyltransferase and amylo-1,6-glucosidase
CC in glycogen degradation. {ECO:0000256|ARBA:ARBA00003530}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of (1->6)-alpha-D-glucosidic branch linkages in
CC glycogen phosphorylase limit dextrin.; EC=3.2.1.33;
CC Evidence={ECO:0000256|ARBA:ARBA00000927};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Transfers a segment of a (1->4)-alpha-D-glucan to a new
CC position in an acceptor, which may be glucose or a (1->4)-alpha-D-
CC glucan.; EC=2.4.1.25; Evidence={ECO:0000256|ARBA:ARBA00000439};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- SIMILARITY: Belongs to the glycogen debranching enzyme family.
CC {ECO:0000256|ARBA:ARBA00025780}.
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DR EMBL; GL533036; EFQ94963.1; -; Genomic_DNA.
DR RefSeq; XP_003296942.1; XM_003296894.1.
DR STRING; 861557.E3RH37; -.
DR EnsemblFungi; EFQ94963; EFQ94963; PTT_07191.
DR KEGG; pte:PTT_07191; -.
DR eggNOG; KOG3625; Eukaryota.
DR HOGENOM; CLU_001517_2_0_1; -.
DR OrthoDB; 1427975at2759; -.
DR Proteomes; UP000001067; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004134; F:4-alpha-glucanotransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0004135; F:amylo-alpha-1,6-glucosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0102500; F:beta-maltose 4-alpha-glucanotransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0005978; P:glycogen biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0005980; P:glycogen catabolic process; IEA:InterPro.
DR CDD; cd11327; AmyAc_Glg_debranch_2; 1.
DR Gene3D; 1.50.10.10; -; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 2.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR010401; AGL/Gdb1.
DR InterPro; IPR032788; AGL_central.
DR InterPro; IPR029436; AGL_euk_N.
DR InterPro; IPR032792; AGL_glucanoTrfase.
DR InterPro; IPR032790; GDE_C.
DR InterPro; IPR006421; Glycogen_debranch_met.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR NCBIfam; TIGR01531; glyc_debranch; 1.
DR PANTHER; PTHR10569; GLYCOGEN DEBRANCHING ENZYME; 1.
DR PANTHER; PTHR10569:SF2; GLYCOGEN DEBRANCHING ENZYME; 1.
DR Pfam; PF06202; GDE_C; 1.
DR Pfam; PF14701; hDGE_amylase; 1.
DR Pfam; PF14702; hGDE_central; 1.
DR Pfam; PF14699; hGDE_N; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Glycogen biosynthesis {ECO:0000256|ARBA:ARBA00023056};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW Glycosyltransferase {ECO:0000256|ARBA:ARBA00022676};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW Reference proteome {ECO:0000313|Proteomes:UP000001067};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 49..143
FT /note="Eukaryotic glycogen debranching enzyme N-terminal"
FT /evidence="ECO:0000259|Pfam:PF14699"
FT DOMAIN 147..592
FT /note="Glycogen debranching enzyme glucanotransferase"
FT /evidence="ECO:0000259|Pfam:PF14701"
FT DOMAIN 753..997
FT /note="Glycogen debranching enzyme central"
FT /evidence="ECO:0000259|Pfam:PF14702"
FT DOMAIN 1087..1542
FT /note="Glycogen debranching enzyme C-terminal"
FT /evidence="ECO:0000259|Pfam:PF06202"
SQ SEQUENCE 1569 AA; 177331 MW; D1E6006A6D9843C6 CRC64;
MAVALPHHSK SSSPRKVVYL LPLTDYGCPD VAGTYIYMPP PTDPAYSICF QIQGTSSICR
EGSLWVNIPA KGEKFERSNY REYKLQPNFT GLCEIEIPIH EANAFSFYTT YSPLPQWSFT
DVASPKPTRT DTYYIDVCPS LRLKGEHLPV EAVCIFSTLS KFMGNYPTDW DNHLRGISQR
GYNMVHFTPL MMRGSSNSPY SIYDQLKFDD AIFKNGEQDI ADMVQKMHSE FNLLAMTDVV
WNHTANNSPW LEEHPEAGYN VDTAPHLRPA LELDTALLQF GQELAERGLP TTFNNEADLL
KVMQGIKAHV LSKVKLWEYY VLDVERDTKA TMEAWISGQV NFVDGSFSEA GLRGLDAVKD
WPLKQKADWL VEHALRGGDR MGERFRRTMD PNISASLLCA LFGRFDTRTS DKPDERAAQG
TMTAIFNEVN LPFYREYDGD QSEILEQLFN RIKYVRIDAH GPKLGEVTEA NPLIETYFTR
LPLNDTTKKH NPEALALVNN GWVWAADAMR DNAGPKSRAY LRREVIVWGD CVKLRYGNGP
EDNPFLWEHM AKYTRLMAKY FQGFRIDNCH STPIHLAEYM LDQARSVNSN IMVCAELFTG
SEEMDYKFCM KLGICALIRE AMQSWSTQEM SRLVHRHGGV PIGSFETDEV MNAAQATDGS
DQTKPIIKKI KRSPVHALFM DCTHDNETPA QKRDARDTLP SAALVAMCDC ATGSVFGFDE
VYPELIELVH EKRLYSSANS TGGPIKPQAG EGGIGGIRKI INEIHVKMGR EEYNETYIHH
DNEYITVHRV NPHTRKGYFL IAHTAFPGYG NGNGGFGKTN LPGTQAKLIG AWNLEVDDSP
ETKARIIGDK TTLRGLPSKT NNLQGVNIEL SGDDTTITIP DKFPPGSIAL FETWVPSAEH
ADGLDKFVTS GARTAFSGVN LTDLNYILYR CEEEERDATD HKDGTYNIPG HGNLVYAGLQ
GWWSVLRDIV NDNNLGHPLC NHLREGQWAL DYCLGRLEHI SQKDNYANIK GPAKWLKSKF
DAIRKVPSYM LPRYFALVIQ TAYNAAVERA IELMGENVRE GNQFLKSLAL VSCQVTGYMN
SASLWPEKSV PSMAAGLPHF AYDWARCWGR DITISARGLY MGTGRYDDAK EHIYAFASVL
KHGMVPNLLG GGKNPRYNSR DSVWWFLQNI QDYTKIVPKG MELLKDQVKR RFLPYDDTWF
AHTDKQAYSK SSTIEDVIQE ALQRHASGIE FREYDAGPNI DSQMKSEGFN IKIWTDWETG
LIFGGNQWNC GTWMDKMGES EKAGSKGVPG TPRDGAPVEI IGMLYSTVRW CANMYEAGQF
KYEGVTLDDG KKTVTYKEWA DLIKANFERC FYVPRSAEED SKYDVNSAIV NRRGIYKDLY
RSGKEYEDYQ LRPNFPVTMT VAPDLFDPEH ALYALQMADR VLLGPQGMKT LDPSDLNYRG
YYINSEDSTD FHTSKGRNYH QGPEWVWPTG FFLRALLKFD LKRRKTPEER IESYQQVTRR
LAGCMKAIQE SPWAGLTELT NKDGAFCGDS CPTQSWSASC IIDLFQDARE YEMEGSGTGA
SKSKKQPSS
//
