ID E6KWU9_9PAST Unreviewed; 1108 AA.
AC E6KWU9;
DT 08-MAR-2011, integrated into UniProtKB/TrEMBL.
DT 08-MAR-2011, sequence version 1.
DT 24-JAN-2024, entry version 46.
DE SubName: Full=MscS family small conductance mechanosenstive potassium channel KefA {ECO:0000313|EMBL:EFU67980.1};
GN Name=kefA {ECO:0000313|EMBL:EFU67980.1};
GN ORFNames=HMPREF9064_0643 {ECO:0000313|EMBL:EFU67980.1};
OS Aggregatibacter segnis ATCC 33393.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
OC Pasteurellaceae; Aggregatibacter.
OX NCBI_TaxID=888057 {ECO:0000313|EMBL:EFU67980.1, ECO:0000313|Proteomes:UP000032871};
RN [1] {ECO:0000313|EMBL:EFU67980.1, ECO:0000313|Proteomes:UP000032871}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 33393 {ECO:0000313|EMBL:EFU67980.1,
RC ECO:0000313|Proteomes:UP000032871};
RA Muzny D., Qin X., Deng J., Jiang H., Liu Y., Qu J., Song X.-Z., Zhang L.,
RA Thornton R., Coyle M., Francisco L., Jackson L., Javaid M., Korchina V.,
RA Kovar C., Mata R., Mathew T., Ngo R., Nguyen L., Nguyen N., Okwuonu G.,
RA Ongeri F., Pham C., Simmons D., Wilczek-Boney K., Hale W., Jakkamsetti A.,
RA Pham P., Ruth R., San Lucas F., Warren J., Zhang J., Zhao Z., Zhou C.,
RA Zhu D., Lee S., Bess C., Blankenburg K., Forbes L., Fu Q., Gubbala S.,
RA Hirani K., Jayaseelan J.C., Lara F., Munidasa M., Palculict T., Patil S.,
RA Pu L.-L., Saada N., Tang L., Weissenberger G., Zhu Y., Hemphill L.,
RA Shang Y., Youmans B., Ayvaz T., Ross M., Santibanez J., Aqrawi P.,
RA Gross S., Joshi V., Fowler G., Nazareth L., Reid J., Worley K.,
RA Petrosino J., Highlander S., Gibbs R.;
RL Submitted (DEC-2010) to the EMBL/GenBank/DDBJ databases.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the MscS (TC 1.A.23) family.
CC {ECO:0000256|ARBA:ARBA00008017}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EFU67980.1}.
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DR EMBL; AEPS01000003; EFU67980.1; -; Genomic_DNA.
DR RefSeq; WP_006717631.1; NZ_LS483443.1.
DR AlphaFoldDB; E6KWU9; -.
DR STRING; 739.GCA_001059425_01774; -.
DR GeneID; 60800040; -.
DR HOGENOM; CLU_007829_3_0_6; -.
DR OrthoDB; 9799209at2; -.
DR Proteomes; UP000032871; Unassembled WGS sequence.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-KW.
DR GO; GO:0034220; P:monoatomic ion transmembrane transport; IEA:UniProtKB-KW.
DR Gene3D; 1.10.287.1260; -; 1.
DR Gene3D; 2.30.30.60; -; 1.
DR Gene3D; 3.30.70.100; -; 1.
DR InterPro; IPR010920; LSM_dom_sf.
DR InterPro; IPR049142; MS_channel_1st.
DR InterPro; IPR049278; MS_channel_C.
DR InterPro; IPR023408; MscS_beta-dom_sf.
DR InterPro; IPR006685; MscS_channel_2nd.
DR InterPro; IPR011066; MscS_channel_C_sf.
DR InterPro; IPR006686; MscS_channel_CS.
DR InterPro; IPR011014; MscS_channel_TM-2.
DR InterPro; IPR025692; MscS_IM_dom1.
DR InterPro; IPR024393; MscS_porin.
DR PANTHER; PTHR30347:SF8; MECHANOSENSITIVE CHANNEL MSCK; 1.
DR PANTHER; PTHR30347; POTASSIUM CHANNEL RELATED; 1.
DR Pfam; PF21088; MS_channel_1st; 1.
DR Pfam; PF00924; MS_channel_2nd; 1.
DR Pfam; PF21082; MS_channel_3rd; 1.
DR Pfam; PF12795; MscS_porin; 1.
DR Pfam; PF12794; MscS_TM; 1.
DR SUPFAM; SSF82689; Mechanosensitive channel protein MscS (YggB), C-terminal domain; 1.
DR SUPFAM; SSF82861; Mechanosensitive channel protein MscS (YggB), transmembrane region; 1.
DR SUPFAM; SSF50182; Sm-like ribonucleoproteins; 1.
DR PROSITE; PS01246; UPF0003; 1.
PE 3: Inferred from homology;
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Ion channel {ECO:0000313|EMBL:EFU67980.1};
KW Ion transport {ECO:0000313|EMBL:EFU67980.1};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000032871};
KW Signal {ECO:0000256|SAM:SignalP};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}; Transport {ECO:0000313|EMBL:EFU67980.1}.
FT SIGNAL 1..26
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 27..1108
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5003207343"
FT TRANSMEM 486..506
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 535..562
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 568..589
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 616..633
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 645..663
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 684..707
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 787..807
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 838..857
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 878..899
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 911..939
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 45..271
FT /note="Mechanosensitive ion channel MscS porin"
FT /evidence="ECO:0000259|Pfam:PF12795"
FT DOMAIN 492..823
FT /note="Mechanosensitive ion channel inner membrane"
FT /evidence="ECO:0000259|Pfam:PF12794"
FT DOMAIN 884..925
FT /note="Mechanosensitive ion channel transmembrane helices
FT 2/3"
FT /evidence="ECO:0000259|Pfam:PF21088"
FT DOMAIN 927..991
FT /note="Mechanosensitive ion channel MscS"
FT /evidence="ECO:0000259|Pfam:PF00924"
FT DOMAIN 1000..1083
FT /note="Mechanosensitive ion channel MscS C-terminal"
FT /evidence="ECO:0000259|Pfam:PF21082"
FT COILED 72..140
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 188..267
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 1108 AA; 124527 MW; F155CED8E68858EA CRC64;
MKIKTFLTAL CAALFLNMAV SQSAFADQNT LSLPTEKSLK ADLAAAQKMP DGDEKQTLVT
DIQTSLDLLQ QIQAQQKANE ALQATLNGAE AEIQKNNAEL QTLKKQLNTA TAEDYQSRHL
ADLQAQLEKL IEQQQETQSA LGAVNSQLAG QSSVSERAQA TLTANLKRAQ ELNQKLGDST
TSAALKQQAQ LELQLIDLKN NYNQILLKNS DQLTVLYQSR YDLLNARAQV LQQQIAAIQE
AINQQHLAQT QNQVDQVQQQ SQSAVKNNYI QKELERNAQL SQFLLEQTEK TNALTQDELR
MRSVLDSLTQ TQRTIDEQIS ALQGTLVLSR IIQQQKQKLP TNLNIQELSK QIADLRVQIF
DMTQRRNELY DLDAYLAKVQ ADQNQTFTEA EKTQLINLLT ERRKIASDVI KSLNNQLNLA
ISLELTQQQI TQISDQIQAK LDQQSFWVKS NNPINLAWFK NLPMSLGEQL EGISKKLGFP
SNFDNLPYLL TYVAILVLIG GIIFRFKTFI KQKLASINSE INRLRTDSQW HTPMALLFTG
IISLSSTLWF LAVCQLFGFF FVRNPAEFWQ WSLSMAAYWW FFTFVLAVLR PNGILVHHFG
FTQDAAEKLQ AITKRIIVTV VLLLNTSVFS DVMESGLADD VLGEVNTIIA LVFCIVIIAP
RFSKASKAFG QTSHDHRDKL LLKIVRILLQ LVPVGLIVLV VLGYYYTALN LINHMINTYI
AWVVWSLARH TIYRAITVSS QRLAYRRLQE KRQQKAAELN DGSPSDDVVV VSEQEEGMAV
NQVRSQLLRF ADLFIWTALF AILYYVWSDL VTVASYLRDI TLWQQTTTTE AGVVTESISL
FNLLVALVII GITYILVRNL PGILEVLIFS RVKLSQGTPY TINTLLTYTL VAIGGAWAFA
TLGMSWSKLQ WLFAALSVGL GFGMQEIFAN FVSGIILLFE RPIRVGDTVT INDVTGTVAK
IRIRAITIID PDRKEVIVPN KSFVTGQVIN WALSNTITRV VISIGVAYGS DLDLVKRLLL
KAASEQANIM KDPEPRALFL SFGASTLDHE LRVYVSQVSE RTNTIDALNR RINELFAENN
IEIAFNQLDV FIKNQDTGAI MPLVEVKS
//
