ID E6RD51_CRYGW Unreviewed; 813 AA.
AC E6RD51;
DT 08-MAR-2011, integrated into UniProtKB/TrEMBL.
DT 08-MAR-2011, sequence version 1.
DT 24-JAN-2024, entry version 61.
DE RecName: Full=alpha-1,2-Mannosidase {ECO:0000256|RuleBase:RU361193};
DE EC=3.2.1.- {ECO:0000256|RuleBase:RU361193};
GN OrderedLocusNames=CGB_K0370C {ECO:0000313|EMBL:ADV24786.1};
OS Cryptococcus gattii serotype B (strain WM276 / ATCC MYA-4071)
OS (Filobasidiella gattii) (Cryptococcus bacillisporus).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes;
OC Tremellales; Cryptococcaceae; Cryptococcus;
OC Cryptococcus gattii species complex.
OX NCBI_TaxID=367775 {ECO:0000313|EMBL:ADV24786.1, ECO:0000313|Proteomes:UP000007805};
RN [1] {ECO:0000313|EMBL:ADV24786.1, ECO:0000313|Proteomes:UP000007805}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=WM276 / ATCC MYA-4071 {ECO:0000313|Proteomes:UP000007805};
RX PubMed=21304167; DOI=10.1128/mBio.00342-10;
RA D'Souza C.A., Kronstad J.W., Taylor G., Warren R., Yuen M., Hu G.,
RA Jung W.H., Sham A., Kidd S.E., Tangen K., Lee N., Zeilmaker T., Sawkins J.,
RA McVicker G., Shah S., Gnerre S., Griggs A., Zeng Q., Bartlett K., Li W.,
RA Wang X., Heitman J., Stajich J.E., Fraser J.A., Meyer W., Carter D.,
RA Schein J., Krzywinski M., Kwon-Chung K.J., Varma A., Wang J., Brunham R.,
RA Fyfe M., Ouellette B.F., Siddiqui A., Marra M., Jones S., Holt R.,
RA Birren B.W., Galagan J.E., Cuomo C.A.;
RT "Genome variation in Cryptococcus gattii, an emerging pathogen of
RT immunocompetent hosts.";
RL MBio 2:E342-E342(2011).
RN [2]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=WM276;
RA D'Souza C.A., Kronstad J.W., Taylor G., Warren R., Yuen M., Hu G.,
RA Jung W.H., Sham A., Kidd S.E., Tangen K., Lee N., Zeilmaker T., Sawkins J.,
RA McVicker G., Shah S., Gnerre S., Griggs A., Zeng Q., Bartlett K., Li W.,
RA Wang X., Heitman J., Stajich J.E., Fraser J.A., Meyer W., Carter D.,
RA Schein J., Krzywinski M., Kwong-Chung K.J., Varma A., Wang J., Brunham R.,
RA Fyfe M., Ouellette B.F.F., Siddiqui A., Marra M., Jones S., Holt R.,
RA Birren B.W., Galagan J.E., Cuomo C.A.;
RT "Genome variation in Cryptococcus gattii, an emerging pathogen of
RT immunocompetent hosts.";
RL MBio 0:0-0(2011).
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000256|PIRSR:PIRSR601382-2};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 47 family.
CC {ECO:0000256|ARBA:ARBA00007658, ECO:0000256|RuleBase:RU361193}.
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DR EMBL; CP000296; ADV24786.1; -; Genomic_DNA.
DR RefSeq; XP_003196573.1; XM_003196525.1.
DR AlphaFoldDB; E6RD51; -.
DR GeneID; 10185062; -.
DR KEGG; cgi:CGB_K0370C; -.
DR VEuPathDB; FungiDB:CGB_K0370C; -.
DR eggNOG; KOG2429; Eukaryota.
DR HOGENOM; CLU_003818_2_1_1; -.
DR OrthoDB; 942598at2759; -.
DR Proteomes; UP000007805; Chromosome K.
DR GO; GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IEA:InterPro.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0004571; F:mannosyl-oligosaccharide 1,2-alpha-mannosidase activity; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR GO; GO:0030433; P:ubiquitin-dependent ERAD pathway; IEA:UniProt.
DR Gene3D; 1.50.10.10; -; 1.
DR Gene3D; 3.50.30.30; -; 1.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR044674; EDEM1/2/3.
DR InterPro; IPR001382; Glyco_hydro_47.
DR InterPro; IPR003137; PA_domain.
DR InterPro; IPR036026; Seven-hairpin_glycosidases.
DR PANTHER; PTHR45679:SF5; ER DEGRADATION-ENHANCING ALPHA-MANNOSIDASE-LIKE PROTEIN 1; 1.
DR PANTHER; PTHR45679; ER DEGRADATION-ENHANCING ALPHA-MANNOSIDASE-LIKE PROTEIN 2; 1.
DR Pfam; PF01532; Glyco_hydro_47; 1.
DR Pfam; PF02225; PA; 1.
DR PRINTS; PR00747; GLYHDRLASE47.
DR SUPFAM; SSF48225; Seven-hairpin glycosidases; 1.
PE 3: Inferred from homology;
KW Calcium {ECO:0000256|PIRSR:PIRSR601382-2};
KW Endoplasmic reticulum {ECO:0000256|ARBA:ARBA00022824};
KW Glycosidase {ECO:0000256|RuleBase:RU361193};
KW Hydrolase {ECO:0000256|RuleBase:RU361193};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR601382-2}.
FT DOMAIN 640..700
FT /note="PA"
FT /evidence="ECO:0000259|Pfam:PF02225"
FT REGION 768..789
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 371
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000256|PIRSR:PIRSR601382-2"
SQ SEQUENCE 813 AA; 89908 MW; DB251E6997693564 CRC64;
MTIRALGGLL SMYQYLDDLP DSPYEQVKVL GIMREGKEGW LLGLGLGAQE KVDKMDVKKY
KQRILELAED LGKRMLPAFN TKTGLPYARV NLRHGVEKGE TVETCTAGAG SLILEFSLLS
RLTGDDRYEQ LARNAYYSIW NRRSDHNLLG NTIGATHGHW LMPGLSGVGA GMDSFFEYGV
KAGIMLGDDS FYDIFYDSYA AIQTHVRTPD GFIYRPIHTR LLQIPSPTTI DSLSAFLPAI
QVLAGDIPSA IRAHLVFWNV WRKFGALPES WRWQERQIEW AGWPGRPEFI ESTYYLYQAT
KDAFYLRVGE RVLKDLARRT KTSCGFATIK NVLTGELEDR MESFMLSETL KYLYLLFSET
PFPNQNKVFT TEGHPLYIPQ PLLQPITNAR KSLHKGEWLT CPAYQTSFAA GGIDFRETDD
VGGRREKAGI IVGIEGTSEY EYARALVFGW GEEGLKVEDQ KRMWFEGGVC SIQEVPKFAF
DIVLTTSSNQ TLPEDPSPGP DKVYQNTTTG DYVINNITGI RLGVRWRLDG QGYDVSIGPH
RVRTGQQVTV IDSSMRNHFP LPSLAPGPPQ PEYEPTEVIL RFVFLSPVIV KDKHLEAGTV
FLHALGATAT FGKDFGASAT SPNKEDQQGW QLDGGALPIL VPPNPSDGCS PLTLSTPDRP
FILLLDRGNC TFAEKAQNTE TVGASGLLIV GYPYPPEGGV TEGDQTVYAV PEEGMLRPSA
EPNEVEGLDS MGIVYMEHLV GELVRNASQK GEVGAAIMRI EGVGEEAAGS SGFPQAGSRA
TKKHLEGDSR TREGRLAVGD WEICNLMIVE TSV
//
