ID E6ZHI0_DICLA Unreviewed; 1390 AA.
AC E6ZHI0;
DT 08-MAR-2011, integrated into UniProtKB/TrEMBL.
DT 08-MAR-2011, sequence version 1.
DT 27-MAR-2024, entry version 44.
DE RecName: Full=DNA-directed RNA polymerase subunit {ECO:0000256|RuleBase:RU004279};
DE EC=2.7.7.6 {ECO:0000256|RuleBase:RU004279};
GN Name=POLR3A {ECO:0000313|EMBL:CBN81514.1};
GN ORFNames=DLA_Ib03560 {ECO:0000313|EMBL:CBN81514.1};
OS Dicentrarchus labrax (European seabass) (Morone labrax).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Eupercaria; Moronidae; Dicentrarchus.
OX NCBI_TaxID=13489 {ECO:0000313|EMBL:CBN81514.1};
RN [1] {ECO:0000313|EMBL:CBN81514.1}
RP NUCLEOTIDE SEQUENCE.
RA Kuhl H., Tine M., Hecht J., Knaust F., Reinhardt R.;
RT "Analysis of single nucleotide polymorphisms in three chromosomes of
RT European sea bass Dicentrarchus labrax.";
RL Comp. Biochem. Physiol. Part D Genomics Proteomics 6:70-75(2011).
RN [2] {ECO:0000313|EMBL:CBN81514.1}
RP NUCLEOTIDE SEQUENCE.
RX DOI=10.1016/j.ygeno.2011.06.004;
RA Kuhl H., Tine M., Beck A., Timmermann B., Kodira C., Reinhardt R.;
RT "Directed sequencing and annotation of three Dicentrarchus labrax L.
RT chromosomes by applying Sanger- and pyrosequencing technologies on pooled
RT DNA of comparatively mapped BAC clones.";
RL Genomics 0:0-0(2011).
RN [3] {ECO:0000313|EMBL:CBN81514.1}
RP NUCLEOTIDE SEQUENCE.
RA Tine M., Kuhl H., Beck A., Bargelloni L., Reinhardt R.;
RT "Comparative analysis of intronless genes in teleost fish genomes: Insights
RT into their evolution and molecular function.";
RL Mar. Genomics 4:109-119(2011).
RN [4] {ECO:0000313|Ensembl:ENSDLAP00005046691.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (SEP-2023) to UniProtKB.
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000256|RuleBase:RU004279}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000256|RuleBase:RU004279};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family.
CC {ECO:0000256|ARBA:ARBA00006460, ECO:0000256|RuleBase:RU004279}.
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DR EMBL; FQ310507; CBN81514.1; -; Genomic_DNA.
DR Ensembl; ENSDLAT00005049813.2; ENSDLAP00005046691.1; ENSDLAG00005020605.2.
DR GeneTree; ENSGT00930000151028; -.
DR Proteomes; UP000694389; Unassembled WGS sequence.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0031981; C:nuclear lumen; IEA:UniProt.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR GO; GO:0006351; P:DNA-templated transcription; IEA:InterPro.
DR CDD; cd02736; RNAP_III_Rpc1_C; 1.
DR CDD; cd02583; RNAP_III_RPC1_N; 1.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.150.390; -; 1.
DR Gene3D; 2.40.40.20; -; 1.
DR Gene3D; 6.10.250.2940; -; 1.
DR Gene3D; 6.20.50.80; -; 1.
DR Gene3D; 3.30.1490.180; RNA polymerase ii; 1.
DR Gene3D; 4.10.860.120; RNA polymerase II, clamp domain; 1.
DR Gene3D; 1.10.274.100; RNA polymerase Rpb1, domain 3; 1.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR000722; RNA_pol_asu.
DR InterPro; IPR006592; RNA_pol_N.
DR InterPro; IPR007080; RNA_pol_Rpb1_1.
DR InterPro; IPR007066; RNA_pol_Rpb1_3.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR044893; RNA_pol_Rpb1_clamp_domain.
DR InterPro; IPR035698; RNAP_III_Rpc1_C.
DR InterPro; IPR035697; RNAP_III_RPC1_N.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; DNA-DIRECTED RNA POLYMERASE; 1.
DR PANTHER; PTHR19376:SF32; DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC1; 1.
DR Pfam; PF04997; RNA_pol_Rpb1_1; 1.
DR Pfam; PF00623; RNA_pol_Rpb1_2; 1.
DR Pfam; PF04983; RNA_pol_Rpb1_3; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 1.
DR SMART; SM00663; RPOLA_N; 1.
DR SUPFAM; SSF64484; beta and beta-prime subunits of DNA dependent RNA-polymerase; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022478,
KW ECO:0000256|RuleBase:RU004279};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU004279}; Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Reference proteome {ECO:0000313|Proteomes:UP000694389};
KW Transcription {ECO:0000256|ARBA:ARBA00023163,
KW ECO:0000256|RuleBase:RU004279};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU004279}.
FT DOMAIN 248..553
FT /note="RNA polymerase N-terminal"
FT /evidence="ECO:0000259|SMART:SM00663"
SQ SEQUENCE 1390 AA; 155823 MW; 89ACE7890CAECD44 CRC64;
MVKEQFRETD VAKKISHICF GMKSAEQMRQ QAHIQVVSKN LYSQDTKHTP LSYGVLDHRM
GTSEKDRPCL TCGKNLADCL GHYGYLDLEL PCFHVGYFKA IIGILQMICK TCSSIMLTKE
DKLQFMDHLK RPNLAYLQKR GLKKKISDKC RKRTICLNCS AFNGPVKKCG LLKIIHEKYK
TTKKVVDPFV SDFMQSFDIA IEHNKMVEPL LTRAQENLNP LVVLNMFKRI PQEDIPLLLM
NPEAGKPADL ILTRLLVPPL CIRPSVVSDL KSGTNEDDLT MKLTEIIFLN DVIKKHRMTG
AKTQMIMEDW DFLQLQCALY INSELSGIPL NMAPKKWTRG FVQRLKGKQG RFRGNLSGKR
VDFSGRTVIS PDPNLRIDEV AVPVHVAKIL TYPERVNKAN LELMRKLVRN GPEVHPGANF
IQNRHTQMKR FLKYGNREKI AQELRFGDVV ERHLIDGDIV LFNRQPSLHK LSIMAHIAKV
KPHRTFRFNE CVCTPYNADF DGDEMNLHLP QTEEAKAEAL VLMGTKANLV TPRNGEPLIA
AIQDFLTGAY LLTLKDTFFD RSKACQIVAS ILVGKDEKIR VTLPRPAIMK PMALWTGKQI
FSVILKPSKE CPVKANLRTK GKQYCGKGED LCYNDSFVVI HNSELMCGSL DKGTLGSGSK
NNIFYILLRD WGQLEAANAM SRLARLAPVF LSNRGFSIGI GDVTPGQGLL KAKHDLLDDG
YKKCDEYIEA LKTGKLQQQP GCTAEETLEA LILRELSVIR DRAGSACLRE LDKSNSPLIM
ALCGSKGSFI NISQMIACVG QQAISGSRVP DGFENRSLPH FEKHSKLPAA KGFVADSFYS
GLTPTEFFFH TMAGREGLVD TAVKTAETGY MQRRLVKSLE DLCSQYDLTV RSSTGDIIQF
IYGGDGLDPA DMEGKDEPLE FKRVLDNIRA VYTCPYEPAL SQNELVLTAD AIMKRADFLC
CRDSFLEEIK KFIKSVSERI KKTRDKYGIN DNGTSEPKVL YQLDRITPTQ LEKYLETCRD
KYMRAQMEPG SAVGALCAQS IGEPGTQMTL KTFHFAGVAS MNITLGVPRI KEIINASKNI
STPIITANLD VEDDADFARL VKGRIEKTLL GEISEYIEEV FLPDDCFILV KLSLERIRLL
RLEVNAETVR YSICMSKLRV KPGDIAVHGE AVVCVSPREN SKSSMYYVLQ SLKEELPKVV
VQGIPEVARA VIHIDEQSGK NKYKLLVEGD NLRAVMATHG VNGSRTTSNN TYEVEKTLGI
EAARSTIINE IQYTMVNHGM SIDRRHVMLL ADLMSYKGEI LGITRFGLAK MKESVLMLAS
FEKTADHLFD AAYFGQKDSV CGVSECIIMG IPMNIGTGLF KLLHKADKDP SPVKRPLLFD
NADFHIPLIT
//
