ID E7F2G6_DANRE Unreviewed; 345 AA.
AC E7F2G6; A0A0D3LSD6;
DT 08-MAR-2011, integrated into UniProtKB/TrEMBL.
DT 08-MAR-2011, sequence version 1.
DT 27-MAR-2024, entry version 96.
DE SubName: Full=G protein-coupled purinergic receptor p2y 12 {ECO:0000313|EMBL:AHN52243.1};
DE SubName: Full=Purinergic receptor P2Y, G-protein coupled, 12 isoform X1 {ECO:0000313|RefSeq:XP_001921964.1};
DE SubName: Full=Purinergic receptor P2Y12 {ECO:0000313|Ensembl:ENSDARP00000093000, ECO:0000313|RefSeq:NP_001295486.1};
GN Name=p2ry12 {ECO:0000313|Ensembl:ENSDARP00000093000,
GN ECO:0000313|RefSeq:NP_001295486.1, ECO:0000313|RefSeq:XP_001921964.1,
GN ECO:0000313|ZFIN:ZDB-GENE-110208-4};
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955 {ECO:0000313|EMBL:AHN52243.1};
RN [1] {ECO:0000313|Ensembl:ENSDARP00000093000}
RP IDENTIFICATION.
RC STRAIN=Tuebingen {ECO:0000313|Ensembl:ENSDARP00000093000};
RG Ensembl;
RL Submitted (APR-2011) to UniProtKB.
RN [2] {ECO:0000313|RefSeq:NP_001295486.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=22632801;
RA Sieger D., Moritz C., Ziegenhals T., Prykhozhij S., Peri F.;
RT "Long-range Ca2+ waves transmit brain-damage signals to microglia.";
RL Dev. Cell 22:1138-1148(2012).
RN [3] {ECO:0000313|Ensembl:ENSDARP00000093000, ECO:0000313|Proteomes:UP000000437}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen {ECO:0000313|Ensembl:ENSDARP00000093000};
RX PubMed=23594743; DOI=10.1038/nature12111;
RG Genome Reference Consortium Zebrafish;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Eliott D.,
RA Threadgold G., Harden G., Ware D., Begum S., Mortimore B., Mortimer B.,
RA Kerry G., Heath P., Phillimore B., Tracey A., Corby N., Dunn M.,
RA Johnson C., Wood J., Clark S., Pelan S., Griffiths G., Smith M.,
RA Glithero R., Howden P., Barker N., Lloyd C., Stevens C., Harley J.,
RA Holt K., Panagiotidis G., Lovell J., Beasley H., Henderson C., Gordon D.,
RA Auger K., Wright D., Collins J., Raisen C., Dyer L., Leung K.,
RA Robertson L., Ambridge K., Leongamornlert D., McGuire S., Gilderthorp R.,
RA Griffiths C., Manthravadi D., Nichol S., Barker G., Whitehead S., Kay M.,
RA Brown J., Murnane C., Gray E., Humphries M., Sycamore N., Barker D.,
RA Saunders D., Wallis J., Babbage A., Hammond S., Mashreghi-Mohammadi M.,
RA Barr L., Martin S., Wray P., Ellington A., Matthews N., Ellwood M.,
RA Woodmansey R., Clark G., Cooper J., Cooper J., Tromans A., Grafham D.,
RA Skuce C., Pandian R., Andrews R., Harrison E., Kimberley A., Garnett J.,
RA Fosker N., Hall R., Garner P., Kelly D., Bird C., Palmer S., Gehring I.,
RA Berger A., Dooley C.M., Ersan-Urun Z., Eser C., Geiger H., Geisler M.,
RA Karotki L., Kirn A., Konantz J., Konantz M., Oberlander M.,
RA Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G., Osoegawa K., Zhu B.,
RA Rapp A., Widaa S., Langford C., Yang F., Schuster S.C., Carter N.P.,
RA Harrow J., Ning Z., Herrero J., Searle S.M., Enright A., Geisler R.,
RA Plasterk R.H., Lee C., Westerfield M., de Jong P.J., Zon L.I.,
RA Postlethwait J.H., Nusslein-Volhard C., Hubbard T.J., Roest Crollius H.,
RA Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [4] {ECO:0000313|EMBL:AHN52243.1}
RP NUCLEOTIDE SEQUENCE.
RA Li W.;
RT "The ADP Receptor P2ry12 Regulates Endothelial Tip Cell Behaviors through
RT Inhibition of Notch-Flt1 Signaling during Vascular Development.";
RL Submitted (APR-2013) to the EMBL/GenBank/DDBJ databases.
RN [5] {ECO:0000313|RefSeq:NP_001295486.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=27156384;
RA Liu C., Wu C., Yang Q., Gao J., Li L., Yang D., Luo L.;
RT "Macrophages Mediate the Repair of Brain Vascular Rupture through Direct
RT Physical Adhesion and Mechanical Traction.";
RL Immunity 44:1162-1176(2016).
RN [6] {ECO:0000313|RefSeq:NP_001295486.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=28502661;
RA Wakisaka N., Miyasaka N., Koide T., Masuda M., Hiraki-Kajiyama T.,
RA Yoshihara Y.;
RT "An Adenosine Receptor for Olfaction in Fish.";
RL Curr. Biol. 27:1437-1447(2017).
RN [7] {ECO:0000313|RefSeq:NP_001295486.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=28710541;
RA Medrano M.P., Bejarano C.A., Battista A.G., Venera G.D., Bernabeu R.O.,
RA Faillace M.P.;
RT "Injury-induced purinergic signalling molecules upregulate pluripotency
RT gene expression and mitotic activity of progenitor cells in the zebrafish
RT retina.";
RL Purinergic Signal. 13:443-465(2017).
RN [8] {ECO:0000313|RefSeq:NP_001295486.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=29972775;
RA Ferrero G., Mahony C.B., Dupuis E., Yvernogeau L., Di Ruggiero E.,
RA Miserocchi M., Caron M., Robin C., Traver D., Bertrand J.Y., Wittamer V.;
RT "Embryonic Microglia Derive from Primitive Macrophages and Are Replaced by
RT cmyb-Dependent Definitive Microglia in Zebrafish.";
RL Cell Rep. 24:130-141(2018).
RN [9] {ECO:0000313|RefSeq:NP_001295486.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=29465400;
RA Chia K., Mazzolini J., Mione M., Sieger D.;
RT "Tumor initiating cells induce Cxcr4-mediated infiltration of pro-tumoral
RT macrophages into the brain.";
RL Elife 7:e31918-e31918(2018).
RN [10] {ECO:0000313|RefSeq:NP_001295486.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=31076485; DOI=10.1242/dev.174698;
RA Herzog C., Pons Garcia L., Keatinge M., Greenald D., Moritz C., Peri F.,
RA Herrgen L.;
RT "Rapid clearance of cellular debris by microglia limits secondary neuronal
RT cell death after brain injury in vivo. .";
RL Development 146:dev174698-dev174698(2019).
RN [11] {ECO:0000313|RefSeq:NP_001295486.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=30886241;
RA Mitchell D.M., Sun C., Hunter S.S., New D.D., Stenkamp D.L.;
RT "Regeneration associated transcriptional signature of retinal microglia and
RT macrophages.";
RL Sci. Rep. 9:4768-4768(2019).
RN [12] {ECO:0000313|RefSeq:NP_001295486.1, ECO:0000313|RefSeq:XP_001921964.1}
RP IDENTIFICATION.
RC STRAIN=Tuebingen {ECO:0000313|RefSeq:XP_001921964.1};
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
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DR EMBL; CU571333; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; KC987955; AHN52243.1; -; mRNA.
DR RefSeq; NP_001295486.1; NM_001308557.1.
DR RefSeq; XP_001921964.1; XM_001921929.7.
DR STRING; 7955.ENSDARP00000128926; -.
DR PaxDb; 7955-ENSDARP00000128926; -.
DR Ensembl; ENSDART00000102224.5; ENSDARP00000093000.3; ENSDARG00000069945.6.
DR Ensembl; ENSDART00000154780.2; ENSDARP00000128926.1; ENSDARG00000069945.6.
DR GeneID; 100149618; -.
DR KEGG; dre:100149618; -.
DR AGR; ZFIN:ZDB-GENE-110208-4; -.
DR CTD; 64805; -.
DR ZFIN; ZDB-GENE-110208-4; p2ry12.
DR eggNOG; ENOG502QUS2; Eukaryota.
DR HOGENOM; CLU_009579_8_2_1; -.
DR OMA; FVCQVTQ; -.
DR OrthoDB; 5390357at2759; -.
DR TreeFam; TF330969; -.
DR Reactome; R-DRE-392170; ADP signalling through P2Y purinoceptor 12.
DR Reactome; R-DRE-417957; P2Y receptors.
DR Reactome; R-DRE-418594; G alpha (i) signalling events.
DR Proteomes; UP000000437; Chromosome 15.
DR Bgee; ENSDARG00000069945; Expressed in spleen and 2 other cell types or tissues.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0045028; F:G protein-coupled purinergic nucleotide receptor activity; IBA:GO_Central.
DR GO; GO:0007596; P:blood coagulation; IEA:InterPro.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0001774; P:microglial cell activation; IMP:ZFIN.
DR GO; GO:0010725; P:regulation of primitive erythrocyte differentiation; IMP:ZFIN.
DR CDD; cd15150; 7tmA_P2Y12; 1.
DR Gene3D; 1.20.1070.10; Rhodopsin 7-helix transmembrane proteins; 1.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR InterPro; IPR005394; P2Y12_rcpt.
DR PANTHER; PTHR24233:SF0; P2Y PURINOCEPTOR 12; 1.
DR PANTHER; PTHR24233; P2Y PURINOCEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR; 1.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PRINTS; PR01569; P2Y12PRNCPTR.
DR PRINTS; PR01157; P2YPURNOCPTR.
DR SUPFAM; SSF81321; Family A G protein-coupled receptor-like; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 2: Evidence at transcript level;
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Receptor {ECO:0000313|EMBL:AHN52243.1, ECO:0000313|RefSeq:NP_001295486.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000000437};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}.
FT TRANSMEM 33..53
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 65..85
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 105..123
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 146..165
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 198..219
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 240..259
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 45..303
FT /note="G-protein coupled receptors family 1 profile"
FT /evidence="ECO:0000259|PROSITE:PS50262"
FT REGION 1..22
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 345 AA; 39238 MW; BD01E5561BD7A1E7 CRC64;
MEQTTQLSFS NSSVSNSSSC SRDGALKTIV FPVLYSILLI LGLSLNALAA WVFLRIPSKS
HFIIYLKNIV VADIIMTLTF PFKILSDANV ASVGIRIFVC RVSSVLFYLT MYISILFFGL
ISIDRCRKTM WPFVGTNPKR LLHRKLLSGV IWTSLLALSL PNVILTSRPN IGERFKCSDL
KTELGLQWHE VVNHICQVIF WGNLIIVIIC YTLISRELYK SYARTRSPRG TQSKKKHIQV
NVFLVLAVFF ICFVPFHFAR VPYTISQTRA LLFSCPQKLF FFKLKESTLW LSSLNSVLDP
LIYFFLCKSF RSSLFNVMRL APGRCRILRE LGTDSAGDQQ GNALT
//
