ID   E9ASZ4_LEIMU            Unreviewed;       762 AA.
AC   E9ASZ4;
DT   05-APR-2011, integrated into UniProtKB/TrEMBL.
DT   05-APR-2011, sequence version 1.
DT   27-MAR-2024, entry version 46.
DE   RecName: Full=SAM-dependent MTase RsmB/NOP-type domain-containing protein {ECO:0000259|PROSITE:PS51686};
GN   ORFNames=LMXM_36_2170 {ECO:0000313|EMBL:CBZ26068.1};
OS   Leishmania mexicana (strain MHOM/GT/2001/U1103).
OC   Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina;
OC   Trypanosomatida; Trypanosomatidae; Leishmaniinae; Leishmania.
OX   NCBI_TaxID=929439 {ECO:0000313|EMBL:CBZ26068.1, ECO:0000313|Proteomes:UP000007259};
RN   [1] {ECO:0000313|EMBL:CBZ26068.1, ECO:0000313|Proteomes:UP000007259}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MHOM/GT/2001/U1103 {ECO:0000313|EMBL:CBZ26068.1,
RC   ECO:0000313|Proteomes:UP000007259};
RX   PubMed=22038252; DOI=10.1101/gr.122945.111;
RA   Rogers M.B., Hilley J.D., Dickens N.J., Wilkes J., Bates P.A.,
RA   Depledge D.P., Harris D., Her Y., Herzyk P., Imamura H., Otto T.D.,
RA   Sanders M., Seeger K., Dujardin J.C., Berriman M., Smith D.F.,
RA   Hertz-Fowler C., Mottram J.C.;
RT   "Chromosome and gene copy number variation allow major structural change
RT   between species and strains of Leishmania.";
RL   Genome Res. 21:2129-2142(2011).
CC   -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC       superfamily. RsmB/NOP family. {ECO:0000256|PROSITE-ProRule:PRU01023}.
CC   -!- CAUTION: Lacks conserved residue(s) required for the propagation of
CC       feature annotation. {ECO:0000256|PROSITE-ProRule:PRU01023}.
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DR   EMBL; FR799573; CBZ26068.1; -; Genomic_DNA.
DR   RefSeq; XP_003874568.1; XM_003874519.1.
DR   AlphaFoldDB; E9ASZ4; -.
DR   GeneID; 13448022; -.
DR   KEGG; lmi:LMXM_36_2170; -.
DR   VEuPathDB; TriTrypDB:LmxM.36.2170; -.
DR   OMA; QIVHRTR; -.
DR   OrthoDB; 197651at2759; -.
DR   PhylomeDB; E9ASZ4; -.
DR   Proteomes; UP000007259; Chromosome 20.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR   GO; GO:0001510; P:RNA methylation; IEA:InterPro.
DR   Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR   InterPro; IPR049560; MeTrfase_RsmB-F_NOP2_cat.
DR   InterPro; IPR001678; MeTrfase_RsmB-F_NOP2_dom.
DR   InterPro; IPR023267; RCMT.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   PANTHER; PTHR22808; NCL1 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING; 1.
DR   PANTHER; PTHR22808:SF1; RNA CYTOSINE C(5)-METHYLTRANSFERASE NSUN2; 1.
DR   Pfam; PF01189; Methyltr_RsmB-F; 1.
DR   PRINTS; PR02008; RCMTFAMILY.
DR   SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR   PROSITE; PS51686; SAM_MT_RSMB_NOP; 1.
PE   3: Inferred from homology;
KW   Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|PROSITE-
KW   ProRule:PRU01023}; Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW   RNA-binding {ECO:0000256|ARBA:ARBA00022884, ECO:0000256|PROSITE-
KW   ProRule:PRU01023};
KW   S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW   ECO:0000256|PROSITE-ProRule:PRU01023};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PROSITE-
KW   ProRule:PRU01023}.
FT   DOMAIN          100..472
FT                   /note="SAM-dependent MTase RsmB/NOP-type"
FT                   /evidence="ECO:0000259|PROSITE:PS51686"
FT   ACT_SITE        365
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT   BINDING         249
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT   BINDING         278
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT   BINDING         312
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
SQ   SEQUENCE   762 AA;  84244 MW;  21837C79C47EA299 CRC64;
     MPAPKNKLKS FGKNRREIRS ATTTYDDNGN WVGTPHQDKM RLVANKLFDN YYNTLQQLGR
     VVARTVYPTS TDACVSAASS SCAAAQDVSA DVAASLWMRE MELFRTPLLT TCWINDTDPL
     AAHVRAYMES LDSSLLEPIS WYPIAGMGWR ILADKAGFRK RPEMQPLRQY LIKQTALGTI
     SRQEEVSMIP PFLLDIQPND VCLDMCASPG SKTAQMLVAL GRHKVVPFDS DASPFPFEYD
     SDGLVIANEL DAKRANMLVH QVKRLRLLFP FTLFTNHDAR YFPEMSLQPQ PVDAEKAAAG
     AEVLRFDKIL CDVVCSGDGT LRKAPHIFKL WSPKEAINLQ KLQIEIALRA CHLLRVGGRL
     VYSTCSLNPV ENEAVVAQIV HRTRGAMRLV DARALLPKLV CAPGMTKWTV TDAKGRVFPA
     PEGNMHEALF PPHTPGGYSS AAVDALDLSL CMRLFPTHCK GGGFFVAVLD KVSEFRLKKL
     EELKAEEKDA STENAVEAAT ERQTIIGTTV PPKREKKETH TVSAGGAEET ADAGVEALEA
     ELTTAHELAK PKSLPPQFVG APAEIQRAII DFYEIPQFPM QLLFVRTAQG ERELRLTPGS
     VCSIVSRMAA QVLEHKTDNV LIVSAGLRVI AFECLDKGWR IVSESAVLFA KLMRRSSRLV
     RVPVSLIQDM VVNGGRLKEK LLESVKDTQL RSRLECLPIG AFLLEIEAPK ALGGVFYSTA
     LRARSRIQLL VDHEDLEGVQ LRLGEDPVKP AEIPEAEKED DA
//