ID F0ZAC6_DICPU Unreviewed; 473 AA.
AC F0ZAC6;
DT 03-MAY-2011, integrated into UniProtKB/TrEMBL.
DT 03-MAY-2011, sequence version 1.
DT 27-MAR-2024, entry version 40.
DE RecName: Full=protein xylosyltransferase {ECO:0000256|ARBA:ARBA00011972};
DE EC=2.4.2.26 {ECO:0000256|ARBA:ARBA00011972};
DE AltName: Full=Peptide O-xylosyltransferase {ECO:0000256|ARBA:ARBA00042865};
GN ORFNames=DICPUDRAFT_93669 {ECO:0000313|EMBL:EGC39100.1};
OS Dictyostelium purpureum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=5786 {ECO:0000313|EMBL:EGC39100.1, ECO:0000313|Proteomes:UP000001064};
RN [1] {ECO:0000313|Proteomes:UP000001064}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=QSDP1 {ECO:0000313|Proteomes:UP000001064};
RX PubMed=21356102; DOI=10.1186/gb-2011-12-2-r20;
RG US DOE Joint Genome Institute (JGI-PGF);
RA Sucgang R., Kuo A., Tian X., Salerno W., Parikh A., Feasley C.L., Dalin E.,
RA Tu H., Huang E., Barry K., Lindquist E., Shapiro H., Bruce D., Schmutz J.,
RA Salamov A., Fey P., Gaudet P., Anjard C., Babu M.M., Basu S.,
RA Bushmanova Y., van der Wel H., Katoh-Kurasawa M., Dinh C., Coutinho P.M.,
RA Saito T., Elias M., Schaap P., Kay R.R., Henrissat B., Eichinger L.,
RA Rivero F., Putnam N.H., West C.M., Loomis W.F., Chisholm R.L., Shaulsky G.,
RA Strassmann J.E., Queller D.C., Kuspa A., Grigoriev I.V.;
RT "Comparative genomics of the social amoebae Dictyostelium discoideum and
RT Dictyostelium purpureum.";
RL Genome Biol. 12:R20.1-R20.23(2011).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-seryl-[protein] + UDP-alpha-D-xylose = 3-O-(beta-D-xylosyl)-
CC L-seryl-[protein] + H(+) + UDP; Xref=Rhea:RHEA:50192, Rhea:RHEA-
CC COMP:9863, Rhea:RHEA-COMP:12567, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:29999, ChEBI:CHEBI:57632, ChEBI:CHEBI:58223,
CC ChEBI:CHEBI:132085; EC=2.4.2.26;
CC Evidence={ECO:0000256|ARBA:ARBA00001814};
CC -!- PATHWAY: Glycan metabolism; chondroitin sulfate biosynthesis.
CC {ECO:0000256|ARBA:ARBA00004840}.
CC -!- PATHWAY: Glycan metabolism; heparan sulfate biosynthesis.
CC {ECO:0000256|ARBA:ARBA00005093}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000256|ARBA:ARBA00004648}; Single-pass type II membrane protein
CC {ECO:0000256|ARBA:ARBA00004648}. Membrane
CC {ECO:0000256|ARBA:ARBA00004606}; Single-pass type II membrane protein
CC {ECO:0000256|ARBA:ARBA00004606}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 14 family. XylT
CC subfamily. {ECO:0000256|ARBA:ARBA00010195}.
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DR EMBL; GL870963; EGC39100.1; -; Genomic_DNA.
DR RefSeq; XP_003284352.1; XM_003284304.1.
DR AlphaFoldDB; F0ZAC6; -.
DR STRING; 5786.F0ZAC6; -.
DR EnsemblProtists; EGC39100; EGC39100; DICPUDRAFT_93669.
DR GeneID; 10510258; -.
DR KEGG; dpp:DICPUDRAFT_93669; -.
DR eggNOG; KOG0799; Eukaryota.
DR InParanoid; F0ZAC6; -.
DR OMA; SAPSICC; -.
DR OrthoDB; 5390370at2759; -.
DR UniPathway; UPA00755; -.
DR UniPathway; UPA00756; -.
DR Proteomes; UP000001064; Unassembled WGS sequence.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0030158; F:protein xylosyltransferase activity; IBA:GO_Central.
DR GO; GO:0030206; P:chondroitin sulfate biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0050650; P:chondroitin sulfate proteoglycan biosynthetic process; IBA:GO_Central.
DR GO; GO:0015012; P:heparan sulfate proteoglycan biosynthetic process; IBA:GO_Central.
DR InterPro; IPR000742; EGF-like_dom.
DR InterPro; IPR003406; Glyco_trans_14.
DR InterPro; IPR043538; XYLT.
DR PANTHER; PTHR46025; XYLOSYLTRANSFERASE OXT; 1.
DR PANTHER; PTHR46025:SF3; XYLOSYLTRANSFERASE OXT; 1.
DR Pfam; PF02485; Branch; 1.
DR PROSITE; PS00022; EGF_1; 1.
PE 3: Inferred from homology;
KW Disulfide bond {ECO:0000256|ARBA:ARBA00023157};
KW Endoplasmic reticulum {ECO:0000256|ARBA:ARBA00022824};
KW Glycoprotein {ECO:0000256|ARBA:ARBA00023180};
KW Glycosyltransferase {ECO:0000256|ARBA:ARBA00022676};
KW Membrane {ECO:0000256|ARBA:ARBA00023136};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000001064};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 17..28
FT /note="EGF-like"
FT /evidence="ECO:0000259|PROSITE:PS00022"
SQ SEQUENCE 473 AA; 56076 MW; 16493C9AFF65CD02 CRC64;
MMGKECSNGG IGFQNECICP YSFSGSMCEN RDEKEQCNSL NLETSMFNLY PEFIFGDNDE
IDKQYSSSVC CWHKYRVNQN YTEKYINEVL VEPTVDNIKE QSKYIKKLLE TYGPWDRNDK
LLFNYLLTSA NKKGKEDYYL KYADPKYSPP VLEPFKITSS NLAFFLLVSD IKMEEIKTLF
TYLYRPKHYY VIHVDNNFND TKKIEELELY LDNLFKKSYE IDHYLTDNYP KNYRVMKDRF
SGSWGSISLV YPEIAAYSIL FDMVEERSAI SRKNETWTHV INLSANDFPV KTVSELEFFL
RLPTNINRNF LETGPNKESE RYTETFLRTK LGNTIAVKYK DSNVCGSPNN NNPMITNTFY
KGKSNEGSQW HFLTYKFAHY IISDFNSIRR LLSLKFAMIP DEFFYQQVRN ESPFYPNEAI
WDTYNYRFIP WDSSKLAVTK EHIEKGFPYV TYFTRKVYKQ DVKDLIIKKL LHQ
//
