ID F1R0Z9_DANRE Unreviewed; 1741 AA.
AC F1R0Z9; A0A8M1N1C6;
DT 03-MAY-2011, integrated into UniProtKB/TrEMBL.
DT 03-MAY-2011, sequence version 1.
DT 27-MAR-2024, entry version 89.
DE SubName: Full=Intersectin 2b {ECO:0000313|Ensembl:ENSDARP00000081545};
DE SubName: Full=Intersectin-2 {ECO:0000313|RefSeq:NP_001003547.2};
GN Name=itsn2b {ECO:0000313|Ensembl:ENSDARP00000081545,
GN ECO:0000313|RefSeq:NP_001003547.2,
GN ECO:0000313|ZFIN:ZDB-GENE-050309-102};
GN Synonyms=im:7149072 {ECO:0000313|RefSeq:NP_001003547.2},
GN si:ch211-175f11.2 {ECO:0000313|RefSeq:NP_001003547.2};
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955 {ECO:0000313|Ensembl:ENSDARP00000081545};
RN [1] {ECO:0000313|Ensembl:ENSDARP00000081545}
RP IDENTIFICATION.
RC STRAIN=Tuebingen {ECO:0000313|Ensembl:ENSDARP00000081545};
RG Ensembl;
RL Submitted (JUL-2011) to UniProtKB.
RN [2] {ECO:0000313|Ensembl:ENSDARP00000081545, ECO:0000313|Proteomes:UP000000437}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen {ECO:0000313|Ensembl:ENSDARP00000081545};
RX PubMed=23594743; DOI=10.1038/nature12111;
RG Genome Reference Consortium Zebrafish;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Eliott D.,
RA Threadgold G., Harden G., Ware D., Begum S., Mortimore B., Mortimer B.,
RA Kerry G., Heath P., Phillimore B., Tracey A., Corby N., Dunn M.,
RA Johnson C., Wood J., Clark S., Pelan S., Griffiths G., Smith M.,
RA Glithero R., Howden P., Barker N., Lloyd C., Stevens C., Harley J.,
RA Holt K., Panagiotidis G., Lovell J., Beasley H., Henderson C., Gordon D.,
RA Auger K., Wright D., Collins J., Raisen C., Dyer L., Leung K.,
RA Robertson L., Ambridge K., Leongamornlert D., McGuire S., Gilderthorp R.,
RA Griffiths C., Manthravadi D., Nichol S., Barker G., Whitehead S., Kay M.,
RA Brown J., Murnane C., Gray E., Humphries M., Sycamore N., Barker D.,
RA Saunders D., Wallis J., Babbage A., Hammond S., Mashreghi-Mohammadi M.,
RA Barr L., Martin S., Wray P., Ellington A., Matthews N., Ellwood M.,
RA Woodmansey R., Clark G., Cooper J., Cooper J., Tromans A., Grafham D.,
RA Skuce C., Pandian R., Andrews R., Harrison E., Kimberley A., Garnett J.,
RA Fosker N., Hall R., Garner P., Kelly D., Bird C., Palmer S., Gehring I.,
RA Berger A., Dooley C.M., Ersan-Urun Z., Eser C., Geiger H., Geisler M.,
RA Karotki L., Kirn A., Konantz J., Konantz M., Oberlander M.,
RA Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G., Osoegawa K., Zhu B.,
RA Rapp A., Widaa S., Langford C., Yang F., Schuster S.C., Carter N.P.,
RA Harrow J., Ning Z., Herrero J., Searle S.M., Enright A., Geisler R.,
RA Plasterk R.H., Lee C., Westerfield M., de Jong P.J., Zon L.I.,
RA Postlethwait J.H., Nusslein-Volhard C., Hubbard T.J., Roest Crollius H.,
RA Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [3] {ECO:0000313|RefSeq:NP_001003547.2}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Singapore {ECO:0000313|RefSeq:NP_001003547.2};
RX PubMed=27189481;
RA Pasquier J., Cabau C., Nguyen T., Jouanno E., Severac D., Braasch I.,
RA Journot L., Pontarotti P., Klopp C., Postlethwait J.H., Guiguen Y.,
RA Bobe J.;
RT "Gene evolution and gene expression after whole genome duplication in fish:
RT the PhyloFish database.";
RL BMC Genomics 17:368-368(2016).
RN [4] {ECO:0000313|RefSeq:NP_001003547.2}
RP IDENTIFICATION.
RC STRAIN=Singapore {ECO:0000313|RefSeq:NP_001003547.2};
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
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DR EMBL; CR628334; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; NP_001003547.2; NM_001003547.2.
DR STRING; 7955.ENSDARP00000081545; -.
DR PaxDb; 7955-ENSDARP00000081545; -.
DR Ensembl; ENSDART00000087111; ENSDARP00000081545; ENSDARG00000054973.
DR Ensembl; ENSDART00000087111.7; ENSDARP00000081545.5; ENSDARG00000054973.7.
DR GeneID; 445153; -.
DR KEGG; dre:445153; -.
DR AGR; ZFIN:ZDB-GENE-050309-102; -.
DR CTD; 445153; -.
DR ZFIN; ZDB-GENE-050309-102; itsn2b.
DR eggNOG; KOG1029; Eukaryota.
DR eggNOG; KOG4305; Eukaryota.
DR HOGENOM; CLU_002819_2_0_1; -.
DR OMA; QINSPPY; -.
DR OrthoDB; 2910300at2759; -.
DR TreeFam; TF324293; -.
DR Proteomes; UP000000437; Alternate scaffold 17.
DR Proteomes; UP000000437; Chromosome 17.
DR Bgee; ENSDARG00000054973; Expressed in mature ovarian follicle and 26 other cell types or tissues.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0097708; C:intracellular vesicle; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0042734; C:presynaptic membrane; IBA:GO_Central.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:InterPro.
DR GO; GO:0060090; F:molecular adaptor activity; IBA:GO_Central.
DR GO; GO:0150007; P:clathrin-dependent synaptic vesicle endocytosis; IBA:GO_Central.
DR GO; GO:0016197; P:endosomal transport; IBA:GO_Central.
DR CDD; cd08375; C2_Intersectin; 1.
DR CDD; cd00052; EH; 2.
DR CDD; cd13264; PH_ITSN; 1.
DR CDD; cd00160; RhoGEF; 1.
DR CDD; cd11994; SH3_Intersectin2_4; 1.
DR CDD; cd11838; SH3_Intersectin_3; 1.
DR Gene3D; 2.60.40.150; C2 domain; 1.
DR Gene3D; 1.20.900.10; Dbl homology (DH) domain; 1.
DR Gene3D; 1.10.238.10; EF-hand; 2.
DR Gene3D; 2.30.29.30; Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB); 1.
DR Gene3D; 2.30.30.40; SH3 Domains; 4.
DR InterPro; IPR000008; C2_dom.
DR InterPro; IPR035892; C2_domain_sf.
DR InterPro; IPR035899; DBL_dom_sf.
DR InterPro; IPR000219; DH-domain.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR InterPro; IPR000261; EH_dom.
DR InterPro; IPR035740; Intersectin-2_SH3_4.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR001849; PH_domain.
DR InterPro; IPR036028; SH3-like_dom_sf.
DR InterPro; IPR001452; SH3_domain.
DR PANTHER; PTHR46006:SF9; INTERSECTIN-2 ISOFORM X1; 1.
DR PANTHER; PTHR46006; RHO GUANINE NUCLEOTIDE EXCHANGE FACTOR AT 64C, ISOFORM A; 1.
DR Pfam; PF00168; C2; 1.
DR Pfam; PF12763; EF-hand_4; 2.
DR Pfam; PF16652; PH_13; 1.
DR Pfam; PF00621; RhoGEF; 1.
DR Pfam; PF00018; SH3_1; 1.
DR Pfam; PF07653; SH3_2; 2.
DR Pfam; PF14604; SH3_9; 1.
DR PRINTS; PR00499; P67PHOX.
DR PRINTS; PR00452; SH3DOMAIN.
DR SMART; SM00239; C2; 1.
DR SMART; SM00054; EFh; 2.
DR SMART; SM00027; EH; 2.
DR SMART; SM00233; PH; 1.
DR SMART; SM00325; RhoGEF; 1.
DR SMART; SM00326; SH3; 4.
DR SUPFAM; SSF49562; C2 domain (Calcium/lipid-binding domain, CaLB); 1.
DR SUPFAM; SSF48065; DBL homology domain (DH-domain); 1.
DR SUPFAM; SSF47473; EF-hand; 2.
DR SUPFAM; SSF50729; PH domain-like; 1.
DR SUPFAM; SSF50044; SH3-domain; 4.
DR PROSITE; PS50004; C2; 1.
DR PROSITE; PS50010; DH_2; 1.
DR PROSITE; PS00018; EF_HAND_1; 1.
DR PROSITE; PS50222; EF_HAND_2; 2.
DR PROSITE; PS50031; EH; 2.
DR PROSITE; PS50003; PH_DOMAIN; 1.
DR PROSITE; PS50002; SH3; 4.
PE 1: Evidence at protein level;
KW Calcium {ECO:0000256|ARBA:ARBA00022837};
KW Endocytosis {ECO:0000256|ARBA:ARBA00022583};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Proteomics identification {ECO:0007829|PeptideAtlas:F1R0Z9};
KW Reference proteome {ECO:0000313|Proteomes:UP000000437};
KW SH3 domain {ECO:0000256|ARBA:ARBA00022443, ECO:0000256|PROSITE-
KW ProRule:PRU00192}.
FT DOMAIN 14..102
FT /note="EH"
FT /evidence="ECO:0000259|PROSITE:PS50031"
FT DOMAIN 46..81
FT /note="EF-hand"
FT /evidence="ECO:0000259|PROSITE:PS50222"
FT DOMAIN 239..328
FT /note="EH"
FT /evidence="ECO:0000259|PROSITE:PS50031"
FT DOMAIN 272..307
FT /note="EF-hand"
FT /evidence="ECO:0000259|PROSITE:PS50222"
FT DOMAIN 934..995
FT /note="SH3"
FT /evidence="ECO:0000259|PROSITE:PS50002"
FT DOMAIN 1026..1084
FT /note="SH3"
FT /evidence="ECO:0000259|PROSITE:PS50002"
FT DOMAIN 1098..1162
FT /note="SH3"
FT /evidence="ECO:0000259|PROSITE:PS50002"
FT DOMAIN 1172..1231
FT /note="SH3"
FT /evidence="ECO:0000259|PROSITE:PS50002"
FT DOMAIN 1254..1440
FT /note="DH"
FT /evidence="ECO:0000259|PROSITE:PS50010"
FT DOMAIN 1479..1588
FT /note="PH"
FT /evidence="ECO:0000259|PROSITE:PS50003"
FT DOMAIN 1596..1712
FT /note="C2"
FT /evidence="ECO:0000259|PROSITE:PS50004"
FT REGION 205..236
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 375..409
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 678..909
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1075..1101
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 205..227
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 678..903
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1080..1094
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1741 AA; 201671 MW; F5EABB1F837B5074 CRC64;
MNGGRSIWAI TPEERDKHDQ KFDSLLPAQG YVTGEKARNF FIQSGLPQPV LAAIWALADM
NKDGKMDRLE FSIAMKLIKM KLQGQNLPTA LPIIMKQPPV PAPTMPAAPL GMGAMPNVPM
MPNVPILTPI PIATMSPIPV MTPMMGTGLP VSMMPTISSQ TLPNGPIAIL QPSPMPLTST
LPLSSSFSAS PLGFSTTSMN KTSSLLDLGS SSSNSSPSTS LASNSPKGGP SDWGVPQASR
LKYRQQFNSL DKLMSGYLTG PQVRTAMATT LLTQTQLAAI WNLADVDKDG KLKADEFILA
MHLVDMAKMG QPLPLALPSD LVPPSLRGKL GESMNGTTPS IYAGLAEDLC EPEPPQKSRN
NLTFEDKFKK NMELGNAELE KRRQVLQEQQ RREEERRAQK AREEQERRER EARELELKRK
REEEIRLERQ RELERQREEE RLKELERKEA AKKELERQQQ LEWERRRKQE LQNQKSEEQE
DIIKLRAKKR SLEMELEAVG NKQKQISDKL RDAQGRKRIH KNELEMINQK RDSCITEISS
LQKQLEDFKR KLGQSTSEQQ KLTDKLRSLS LNSVPTATVK TLQNNMEEKD LSCRKLKEQL
NAVEKETAAK LADMDQYKKD IQDLRESQRM QESALEKLRT IKEEKIRELK KRKQEELEKK
RREEEEQKRI KLEKERQWQE KLRRDEEEKQ RKIQEERDAK LREEEERERQ ARLQAAKEQA
ERERKAREEE ERRRREEEEE RREQERRRLE EEDRRRRDEE RRKLEEERRK LEEERRKLED
ERKRQEEIRL KEEREREERR RAEEERRRKE EERREEERKR EREEEERRRH RAQEAAMRDA
EERKRIEEER KRKEEERRKQ EEEDRRRREE EQRKQDEERK RKQQQEEAAA ARLRDDEQNK
KNTVKSDLVS ALLRGLEERK GGRRPLGTQH RRSAALTTFK ALYPFTARNE EELSFESDDL
IEVDESVERE QGWLYGSWQG KMGWFPESYV EKQTKSEAPV VAKQALKPQV SISVSSTDAS
DTTDGSNYEE YVALYTYESP EPGDLTFNEG DTILVSEREG EWWRGSIGDR AGLFPSNYVK
PKETDTSSLS GKSGTPGKKP EVAQVTTACT AAGAEQLNLS PGQLILILNK NPSGWWLGEL
QARGKKRQKG WFPASHVKLL GSNSGKSTPA TPSVGQVIAI YDYTAANEDE LSFSKSQVIN
VLDKSNPDWW KGELNGVTGL IPTNYVKLTT DSDPSQQWCA DQSTLESMSP KDRKRQDYIH
ELIDTEEKYV EDLQLVLEVF YKPMSESNRL SEAEMNMIFA NWRELIQCSS KMLKALKARK
KSGGDNMPVH MIGDILTSEL SNMQAYIRFC SCQLEGAALL QQRTDQEPDF KTFLKKIATD
YRCKGMPLSS FLLKPMQRIT RYPLHIKHIL ENTPESHVDY IQLTEALEKA ELLCFEVNEG
VREKENSDRL EWIQSHVQCE GIAENLIFNS MTNCLGPRKL LHSGKVFKMK SNKELHAFLF
SDFLLFTQAV RQFTSSGTDK LFSPKSNTQY KMYKTPVFLN EVLVKLPSDP SSDEPVFHIS
HIDRVYTLKT ENINERTAWV QKIKVASEGF IETEKKKHEK AYQSRSMKGS GIGRLLVTIL
EATELKSSKP NGKSNPYCEV TMGSQCYTSR TINDTVNPKW NFNCQFHIKD LYQDVLCITI
NEKELFSPDA FLGRTEVPVA TIKKELENKG PSTRRLLLHE VPTGEVWVRL DLQLFDHKTP
K
//
