ID F2K7E5_PSEBN Unreviewed; 1257 AA.
AC F2K7E5;
DT 31-MAY-2011, integrated into UniProtKB/TrEMBL.
DT 31-MAY-2011, sequence version 1.
DT 27-MAR-2024, entry version 64.
DE RecName: Full=nitrate reductase (quinone) {ECO:0000256|ARBA:ARBA00012500};
DE EC=1.7.5.1 {ECO:0000256|ARBA:ARBA00012500};
GN ORFNames=PSEBR_a3148 {ECO:0000313|EMBL:AEA69456.1};
OS Pseudomonas brassicacearum (strain NFM421).
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=994484 {ECO:0000313|EMBL:AEA69456.1, ECO:0000313|Proteomes:UP000006692};
RN [1] {ECO:0000313|EMBL:AEA69456.1, ECO:0000313|Proteomes:UP000006692}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NFM421 {ECO:0000313|EMBL:AEA69456.1,
RC ECO:0000313|Proteomes:UP000006692};
RX PubMed=21515771; DOI=10.1128/JB.00411-11;
RA Ortet P., Barakat M., Lalaouna D., Fochesato S., Barbe V., Vacherie B.,
RA Santaella C., Heulin T., Achouak W.;
RT "Complete genome sequence of a beneficial plant root-associated bacterium,
RT Pseudomonas brassicacearum.";
RL J. Bacteriol. 193:3146-3146(2011).
RN [2]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=NFM421;
RA Ortet P., Barakat M., Lalaouna D., Fochesato S., Barbe V., Santaella C.,
RA Heulin T., Achouak W.;
RT "Complete Genome Sequence of a beneficial plant roots-associated bacterium
RT Pseudomonas brassicacearum.";
RL Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a quinol + nitrate = a quinone + H2O + nitrite;
CC Xref=Rhea:RHEA:56144, ChEBI:CHEBI:15377, ChEBI:CHEBI:16301,
CC ChEBI:CHEBI:17632, ChEBI:CHEBI:24646, ChEBI:CHEBI:132124; EC=1.7.5.1;
CC Evidence={ECO:0000256|ARBA:ARBA00001854};
CC -!- COFACTOR:
CC Name=Mo-bis(molybdopterin guanine dinucleotide);
CC Xref=ChEBI:CHEBI:60539; Evidence={ECO:0000256|ARBA:ARBA00001942};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004202};
CC Peripheral membrane protein {ECO:0000256|ARBA:ARBA00004202}. Membrane
CC {ECO:0000256|ARBA:ARBA00004170}; Peripheral membrane protein
CC {ECO:0000256|ARBA:ARBA00004170}.
CC -!- SIMILARITY: Belongs to the prokaryotic molybdopterin-containing
CC oxidoreductase family. {ECO:0000256|ARBA:ARBA00010312}.
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DR EMBL; CP002585; AEA69456.1; -; Genomic_DNA.
DR RefSeq; WP_003202451.1; NC_015379.1.
DR AlphaFoldDB; F2K7E5; -.
DR STRING; 994484.PSEBR_a3148; -.
DR GeneID; 57259844; -.
DR KEGG; pba:PSEBR_a3148; -.
DR HOGENOM; CLU_000422_14_1_6; -.
DR Proteomes; UP000006692; Chromosome.
DR GO; GO:0009325; C:nitrate reductase complex; IEA:InterPro.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0043546; F:molybdopterin cofactor binding; IEA:InterPro.
DR GO; GO:0008940; F:nitrate reductase activity; IEA:InterPro.
DR GO; GO:0045333; P:cellular respiration; IEA:UniProt.
DR GO; GO:0042126; P:nitrate metabolic process; IEA:InterPro.
DR CDD; cd02776; MopB_CT_Nitrate-R-NarG-like; 1.
DR CDD; cd02750; MopB_Nitrate-R-NarG-like; 1.
DR Gene3D; 3.40.50.12440; -; 1.
DR Gene3D; 4.10.1200.10; nitrate reductase tail; 1.
DR InterPro; IPR009010; Asp_de-COase-like_dom_sf.
DR InterPro; IPR037943; MopB_CT_Nitrate-R-NarG-like.
DR InterPro; IPR006657; MoPterin_dinucl-bd_dom.
DR InterPro; IPR006656; Mopterin_OxRdtase.
DR InterPro; IPR006963; Mopterin_OxRdtase_4Fe-4S_dom.
DR InterPro; IPR006655; Mopterin_OxRdtase_prok_CS.
DR InterPro; IPR027467; MopterinOxRdtase_cofactor_BS.
DR InterPro; IPR006468; NarG.
DR InterPro; IPR028189; Nitr_red_alph_N.
DR InterPro; IPR044906; Nitr_red_alph_N_sf.
DR NCBIfam; TIGR01580; narG; 1.
DR PANTHER; PTHR43105; RESPIRATORY NITRATE REDUCTASE; 1.
DR PANTHER; PTHR43105:SF2; RESPIRATORY NITRATE REDUCTASE 2 ALPHA CHAIN; 1.
DR Pfam; PF00384; Molybdopterin; 1.
DR Pfam; PF01568; Molydop_binding; 1.
DR Pfam; PF14710; Nitr_red_alph_N; 1.
DR SMART; SM00926; Molybdop_Fe4S4; 1.
DR SUPFAM; SSF50692; ADC-like; 1.
DR SUPFAM; SSF53706; Formate dehydrogenase/DMSO reductase, domains 1-3; 1.
DR PROSITE; PS51669; 4FE4S_MOW_BIS_MGD; 1.
DR PROSITE; PS00551; MOLYBDOPTERIN_PROK_1; 1.
DR PROSITE; PS00490; MOLYBDOPTERIN_PROK_2; 1.
DR PROSITE; PS00932; MOLYBDOPTERIN_PROK_3; 1.
PE 3: Inferred from homology;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Membrane {ECO:0000256|ARBA:ARBA00022475};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723}.
FT DOMAIN 43..107
FT /note="4Fe-4S Mo/W bis-MGD-type"
FT /evidence="ECO:0000259|PROSITE:PS51669"
SQ SEQUENCE 1257 AA; 140580 MW; 06275E73971E1B5C CRC64;
MSHLLDQLRF FNRKQGEFSD GHGETRKESR DWENVYRSRW QYDKIVRSTH GVNCTGSCSW
KIYVKNGLIT WETQQTDYPR TRNDLPNHEP RGCPRGASYS WYIYSANRLK YPKIRKPLLK
LWREARQTLP PVEAWASIVE DKAKADSYKS KRGMGGFIRS NWEEVNEIIA AANVYTVKEH
GPDRVVGFSP IPAMSMVSYA AGSRYLSLIG GVCLSFYDWY CDLPPASPMV WGEQTDVPES
ADWYNSNYII AWGSNVPQTR TPDAHFFTEV RYKGTKTVAI TPDYSEVAKL TDLWLNPKQG
TDAALAQAFN HVIFKEFHLD KPSVYFTEYA KRYTDLPVLV MLKPMLGAAP GAGYQPDRFL
RASDLTDNLG QDNNPEWKTI AMDAAGELVS PQGSIGYRWG EKGKWNILPR EGGEGREIDL
KLSLIGGDVA EVAFPYFAGE AQEYFQHVAG DAVQFRRVPV HSVVLADGSV AKVATVFDLS
AANLAIDRGL GGANVAKDYD DASVPGTPAW QEQITGVSRE KAIQIAREFA DNADKTRGRS
MIIVGAAMNH WYHMDMNYRG LINMLMLCGC VGQTGGGWAH YVGQEKLRPQ CGWLPLAFGL
DWNRPPRQMN GTSFFYGHSS QWRHEKMSMH DVLSPLADKS QFPEHALDYN IRAERAGWLP
SAPQLNTNPL HICRDAAAAG MDPKDYVVKS LQDGSLRFAC EQPDSPVNFP RNMFIWRSNL
LGSSGKGHEY MLKYLLGTKN GVMNEDIGHS TECKPTEAEW VDEGAIGKLD LVTTLDFRMS
STCVYSDIVL PTATWYEKDD MNTSDMHPFI HPLSAAIDPA WESRSDWEIY KGIAKAFSAM
SVGHLGVEKD LVTVPLMHDS VGELAQPFGG TDWKSAGVAP VPGKNAPNLQ VVERDYPNIY
KQFTSLGPML EKLGNGGKGI NWNTDTEVKF LGELNHKEVE AGISQGRPKI DSAIDAAEVI
LSLAPETNGH VAVKAWAALS EFTGIDHSHL AVSKEHEAIR FRDIQAQPRK IISSPTWSGL
EDDHVSYNAG YTNVHESIPW RTITGRQQFY QDHPWMQAFG EQLMSYRPPV NTRTIAGVKG
KRSNGETEIV LNWITPHQKW GIHSTYSDNL LMLTLSRGGP IVWLSEIDAK RAGIEDNDWI
ECFNVNGALT ARAVVSQRVK EGMVMMYHAQ ERIVNVPGSE TTKTRGGHHN SVTRVVLKPT
HMIGGYAQQA YGFNYYGTVG CNRDEFVVVR KMVKVDWLDG SSGDDLPRPL PTDIEEN
//
