ID F2KMN9_ARCVS Unreviewed; 137 AA.
AC F2KMN9;
DT 31-MAY-2011, integrated into UniProtKB/TrEMBL.
DT 31-MAY-2011, sequence version 1.
DT 27-MAR-2024, entry version 53.
DE RecName: Full=Crossover junction endodeoxyribonuclease Hjc {ECO:0000256|HAMAP-Rule:MF_01490};
DE Short=Hjc {ECO:0000256|HAMAP-Rule:MF_01490};
DE EC=3.1.21.10 {ECO:0000256|HAMAP-Rule:MF_01490};
DE AltName: Full=Holliday junction resolvase Hjc {ECO:0000256|HAMAP-Rule:MF_01490};
GN Name=hjc {ECO:0000256|HAMAP-Rule:MF_01490};
GN OrderedLocusNames=Arcve_1229 {ECO:0000313|EMBL:AEA47236.1};
OS Archaeoglobus veneficus (strain DSM 11195 / SNP6).
OC Archaea; Euryarchaeota; Archaeoglobi; Archaeoglobales; Archaeoglobaceae;
OC Archaeoglobus.
OX NCBI_TaxID=693661 {ECO:0000313|EMBL:AEA47236.1, ECO:0000313|Proteomes:UP000008136};
RN [1] {ECO:0000313|EMBL:AEA47236.1, ECO:0000313|Proteomes:UP000008136}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SNP6;
RG US DOE Joint Genome Institute (JGI-PGF);
RA Lucas S., Copeland A., Lapidus A., Bruce D., Goodwin L., Pitluck S.,
RA Kyrpides N., Mavromatis K., Pagani I., Ivanova N., Mikhailova N., Lu M.,
RA Detter J.C., Tapia R., Han C., Land M., Hauser L., Markowitz V.,
RA Cheng J.-F., Hugenholtz P., Woyke T., Wu D., Spring S., Brambilla E.,
RA Klenk H.-P., Eisen J.A.;
RT "The complete genome of Archaeoglobus veneficus SNP6.";
RL Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: A structure-specific endonuclease that resolves Holliday
CC junction (HJ) intermediates during genetic recombination. Cleaves 4-way
CC DNA junctions introducing paired nicks in opposing strands, leaving a
CC 5'-terminal phosphate and a 3'-terminal hydroxyl group that are
CC subsequently ligated to produce recombinant products.
CC {ECO:0000256|HAMAP-Rule:MF_01490}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endonucleolytic cleavage at a junction such as a reciprocal
CC single-stranded crossover between two homologous DNA duplexes
CC (Holliday junction).; EC=3.1.21.10;
CC Evidence={ECO:0000256|ARBA:ARBA00029354, ECO:0000256|HAMAP-
CC Rule:MF_01490};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_01490};
CC Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000256|HAMAP-Rule:MF_01490};
CC -!- SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_01490}.
CC -!- SIMILARITY: Belongs to the Holliday junction resolvase Hjc family.
CC {ECO:0000256|HAMAP-Rule:MF_01490}.
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DR EMBL; CP002588; AEA47236.1; -; Genomic_DNA.
DR RefSeq; WP_013683898.1; NC_015320.1.
DR AlphaFoldDB; F2KMN9; -.
DR STRING; 693661.Arcve_1229; -.
DR GeneID; 10394347; -.
DR KEGG; ave:Arcve_1229; -.
DR eggNOG; arCOG00919; Archaea.
DR HOGENOM; CLU_139546_0_0_2; -.
DR OrthoDB; 34330at2157; -.
DR Proteomes; UP000008136; Chromosome.
DR GO; GO:0008821; F:crossover junction DNA endonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-UniRule.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR CDD; cd00523; Holliday_junction_resolvase; 1.
DR Gene3D; 3.40.1350.10; -; 1.
DR HAMAP; MF_01490; HJ_Resolv_Hjc; 1.
DR InterPro; IPR002732; Hjc.
DR InterPro; IPR014428; Hjc_arc.
DR InterPro; IPR011335; Restrct_endonuc-II-like.
DR InterPro; IPR011856; tRNA_endonuc-like_dom_sf.
DR NCBIfam; NF040854; Hol_resolv_Hjc; 1.
DR PANTHER; PTHR39651; HOLLIDAY JUNCTION RESOLVASE HJC; 1.
DR PANTHER; PTHR39651:SF1; HOLLIDAY JUNCTION RESOLVASE HJC; 1.
DR Pfam; PF01870; Hjc; 1.
DR PIRSF; PIRSF004985; Hlld_jn_rslvs_ar; 1.
DR SUPFAM; SSF52980; Restriction endonuclease-like; 1.
PE 3: Inferred from homology;
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_01490};
KW DNA recombination {ECO:0000256|ARBA:ARBA00023172, ECO:0000256|HAMAP-
KW Rule:MF_01490};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_01490};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_01490};
KW Endonuclease {ECO:0000256|ARBA:ARBA00022759, ECO:0000256|HAMAP-
KW Rule:MF_01490};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_01490};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842, ECO:0000256|HAMAP-Rule:MF_01490};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723, ECO:0000256|HAMAP-
KW Rule:MF_01490};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722, ECO:0000256|HAMAP-Rule:MF_01490};
KW Reference proteome {ECO:0000313|Proteomes:UP000008136}.
FT ACT_SITE 29
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01490"
FT BINDING 9
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01490"
FT BINDING 38
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01490"
FT BINDING 51
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01490"
FT SITE 53
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01490"
SQ SEQUENCE 137 AA; 15408 MW; E94268C1CEC5AADF CRC64;
MKGKGTRFER ELIHRLWENG FAAVRSAGSG AMSYPMPDIV AGNGKKFLAF EVKMRAKLPL
YLTEQEVKEL VMFSNLFGAE SYIAVKIPRS DWVFVSISQL KRTGKGYKID EDVYAIGSDL
DEVLGRSVQL RLEGKDL
//
