ID F3G532_PSESJ Unreviewed; 216 AA.
AC F3G532;
DT 28-JUN-2011, integrated into UniProtKB/TrEMBL.
DT 28-JUN-2011, sequence version 1.
DT 24-JAN-2024, entry version 48.
DE RecName: Full=tRNA (guanine(46)-N(7))-methyltransferase {ECO:0000256|ARBA:ARBA00011977};
DE EC=2.1.1.33 {ECO:0000256|ARBA:ARBA00011977};
DE Flags: Fragment;
GN Name=trmB {ECO:0000313|EMBL:EGH42182.1};
GN ORFNames=PSYPI_07090 {ECO:0000313|EMBL:EGH42182.1};
OS Pseudomonas syringae pv. pisi str. 1704B.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas; Pseudomonas syringae.
OX NCBI_TaxID=629263 {ECO:0000313|EMBL:EGH42182.1, ECO:0000313|Proteomes:UP000004986};
RN [1] {ECO:0000313|EMBL:EGH42182.1, ECO:0000313|Proteomes:UP000004986}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1704B {ECO:0000313|EMBL:EGH42182.1,
RC ECO:0000313|Proteomes:UP000004986};
RX PubMed=21799664; DOI=10.1371/journal.ppat.1002132;
RA Baltrus D.A., Nishimura M.T., Romanchuk A., Chang J.H., Mukhtar M.S.,
RA Cherkis K., Roach J., Grant S.R., Jones C.D., Dangl J.L.;
RT "Dynamic evolution of pathogenicity revealed by sequencing and comparative
RT genomics of 19 Pseudomonas syringae isolates.";
RL PLoS Pathog. 7:E1002132-e1002132(2011).
CC -!- FUNCTION: Catalyzes the formation of N(7)-methylguanine at position 46
CC (m7G46) in tRNA. {ECO:0000256|ARBA:ARBA00003015}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=guanosine(46) in tRNA + S-adenosyl-L-methionine = N(7)-
CC methylguanosine(46) in tRNA + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:42708, Rhea:RHEA-COMP:10188, Rhea:RHEA-COMP:10189,
CC ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:74269,
CC ChEBI:CHEBI:74480; EC=2.1.1.33;
CC Evidence={ECO:0000256|ARBA:ARBA00000142};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EGH42182.1}.
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DR EMBL; AEAI01000347; EGH42182.1; -; Genomic_DNA.
DR AlphaFoldDB; F3G532; -.
DR PATRIC; fig|629263.4.peg.1172; -.
DR HOGENOM; CLU_050910_0_1_6; -.
DR Proteomes; UP000004986; Unassembled WGS sequence.
DR GO; GO:0008176; F:tRNA (guanine(46)-N7)-methyltransferase activity; IEA:UniProtKB-EC.
DR CDD; cd02440; AdoMet_MTases; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR HAMAP; MF_01057; tRNA_methyltr_TrmB; 1.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR003358; tRNA_(Gua-N-7)_MeTrfase_Trmb.
DR NCBIfam; TIGR00091; tRNA (guanosine(46)-N7)-methyltransferase TrmB; 1.
DR PANTHER; PTHR23417; 3-DEOXY-D-MANNO-OCTULOSONIC-ACID TRANSFERASE/TRNA GUANINE-N 7 - -METHYLTRANSFERASE; 1.
DR PANTHER; PTHR23417:SF14; PPR_LONG DOMAIN-CONTAINING PROTEIN; 1.
DR Pfam; PF02390; Methyltransf_4; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS51625; SAM_MT_TRMB; 1.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000313|EMBL:EGH42182.1};
KW Transferase {ECO:0000313|EMBL:EGH42182.1};
KW tRNA processing {ECO:0000256|ARBA:ARBA00022694}.
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:EGH42182.1"
SQ SEQUENCE 216 AA; 24273 MW; 944A015F421C3678 CRC64;
RERAGRMTEG QQRGLDQGLP LYGLSLTDTP VDFDQVFGRS APRTLEIGFG MGHSLLEMAA
AAPEHDFIGV EVHSPGVGAL LNGVLTQGLT NVRVYDCDAI EVLNRCVADN SLDRLMLFFP
DPWHKSRHHK RRIVQPEFAA LVRSKLKVGG VFHMATDWGP YAEYMLEVMS VAPGYRNQAE
DNQYVPRPAE RPITKFERRG EKLGHGVWDL KFEKVD
//
