ID DEXH3_ARATH Reviewed; 1197 AA.
AC F4HYJ7; Q9C734;
DT 20-JAN-2016, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 27-MAR-2024, entry version 86.
DE RecName: Full=DExH-box ATP-dependent RNA helicase DExH3 {ECO:0000305};
DE EC=3.6.4.13 {ECO:0000305};
GN OrderedLocusNames=At1g48650 {ECO:0000312|Araport:AT1G48650};
GN ORFNames=F11I4_16 {ECO:0000312|EMBL:AAG60124.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY.
RX PubMed=24265739; DOI=10.1371/journal.pone.0078982;
RA Xu R., Zhang S., Huang J., Zheng C.;
RT "Genome-wide comparative in silico analysis of the RNA helicase gene family
RT in Zea mays and Glycine max: a comparison with Arabidopsis and Oryza
RT sativa.";
RL PLoS ONE 8:E78982-E78982(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC Evidence={ECO:0000305};
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=F4HYJ7-1; Sequence=Displayed;
CC -!- SIMILARITY: Belongs to the DExH box helicase family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG60124.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC073555; AAG60124.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE32333.1; -; Genomic_DNA.
DR RefSeq; NP_175298.2; NM_103761.6. [F4HYJ7-1]
DR AlphaFoldDB; F4HYJ7; -.
DR SMR; F4HYJ7; -.
DR STRING; 3702.F4HYJ7; -.
DR iPTMnet; F4HYJ7; -.
DR PaxDb; 3702-AT1G48650-2; -.
DR ProteomicsDB; 224077; -. [F4HYJ7-1]
DR EnsemblPlants; AT1G48650.1; AT1G48650.1; AT1G48650. [F4HYJ7-1]
DR GeneID; 841287; -.
DR Gramene; AT1G48650.1; AT1G48650.1; AT1G48650. [F4HYJ7-1]
DR KEGG; ath:AT1G48650; -.
DR Araport; AT1G48650; -.
DR TAIR; AT1G48650; -.
DR eggNOG; KOG0920; Eukaryota.
DR InParanoid; F4HYJ7; -.
DR OMA; LENIDEW; -.
DR PRO; PR:F4HYJ7; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; F4HYJ7; baseline and differential.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0004386; F:helicase activity; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR CDD; cd17917; DEXHc_RHA-like; 1.
DR CDD; cd00048; DSRM_SF; 1.
DR CDD; cd18791; SF2_C_RHA; 1.
DR Gene3D; 1.20.120.1080; -; 1.
DR Gene3D; 3.30.160.20; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR011709; DEAD-box_helicase_OB_fold.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014720; dsRBD_dom.
DR InterPro; IPR048333; HA2_WH.
DR InterPro; IPR007502; Helicase-assoc_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR18934; ATP-DEPENDENT RNA HELICASE; 1.
DR PANTHER; PTHR18934:SF229; ATP-DEPENDENT RNA HELICASE DHX30; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00035; dsrm; 1.
DR Pfam; PF21010; HA2_C; 1.
DR Pfam; PF04408; HA2_N; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF07717; OB_NTP_bind; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00847; HA2; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF54768; dsRNA-binding domain-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW Alternative splicing; ATP-binding; Helicase; Hydrolase; Nucleotide-binding;
KW Reference proteome; RNA-binding.
FT CHAIN 1..1197
FT /note="DExH-box ATP-dependent RNA helicase DExH3"
FT /id="PRO_0000435293"
FT DOMAIN 309..476
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 564..738
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT MOTIF 423..426
FT /note="DEIH box"
FT /evidence="ECO:0000305"
FT BINDING 322..329
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ SEQUENCE 1197 AA; 134253 MW; 4C93472E210E6283 CRC64;
MQLYSAIFRG HIRPNAVVGA ASMFIQHNSI QLHRSPKLLL RPSSVVRSLH CRRSGGLVTH
SQRSRVLCVK AARGDASSST LGIEWRAANL PYFQRQNSGY GRIAYNDYES SDESDRDVGS
SQSQQMAGST LDNIDQWRFK LTMLLRNKED QEVVSRERKD RRDFDHISAL ATRMGLHSRQ
YSKIVVISKA PLPNYRPDLD DKRPQREVVL PFGLQSEVDA HLHSFLDQKK TLIPEMPRQN
SSESLANGYG NYETPETVMQ NSLARERILR PRSLQLKSKQ QQWVDSPEGQ KMVGFRKTLP
AYKEKDALLK AIAANQVVVV SGETGCGKTT QLPQYILESE IEAARGATCS IICTQPRRIS
AISVSERVAA ERGEQIGESV GYKVRLEGMR GRDTRLLFCT TGVLLRRLLV DRSLKGVTHV
VVDEIHERGM NEDFLLIVLK DLLPRRPDLK LILMSATLNA ELFSSYFGGA PAMHIPGFTY
PVRAHFLEDY LETSGYRLTT YNQIDDYGEE KTWKMQKQAQ FKKRKSLISS AVEDALEAAD
FKGYNFRTRD SLSCWSPDSI GFNLIENVLC HIVKGERPGA VLVFMTGWDD INSLKNQLEA
HSLLGDPNKV LLLACHGSMA SSEQRLIFDR PPEGIRKIVL ATNMAETSIT INDVVYVIDC
GKAKETSYDA LNNTPCLLPS WISKAAARQR RGRAGRVMPG ECYHLYPRCV YEAFADYQQP
ELLRTPLQSL CLQIKSLGLG SISEFLSRAL QPPEALSVQN AVEYLKIIGA LDDDENLTPL
GKNLSMLPVE PKLGKMLILG AIFNCLDPVM TVVAGLSVRD PFLMPFDKKD LAETARSKFS
GRDYSDHLTL VRAYNGWKDA ERTHSGYDYC WKNFLSSQTL KAMDSMRKQF FNLLKEASLI
DNIEGCSKLS HDEHLVRAII CAGMFPGVCS VVNKEKSITL KTMEDGQVLL YSSSVNGNVP
MIPFPWLVFN DKVKVNSVFL RDSTAVSDSV LLLFGDKISS GGFDGHLKML GGYLEFFMKP
TLAYTYLSLK RELDELIQNK LVNPKLDIQL YDKLMTAIRL LVSEDQCEGR FVYGRKALSP
TPAKKLKDVG AQLQNSGGEN NKNQLQTLLA RAGHGSPVYK TRQLKNNQFR SMVTFNGLDF
MGKPCGSKKN AEKDAAHEAL LWLQGESKSS LNDLNHMSML LKKNKSKNHA KASTKWG
//
