ID   F6EPM4_HOYSD            Unreviewed;       815 AA.
AC   F6EPM4;
DT   27-JUL-2011, integrated into UniProtKB/TrEMBL.
DT   27-JUL-2011, sequence version 1.
DT   27-MAR-2024, entry version 49.
DE   SubName: Full=Dimethylglycine oxidase {ECO:0000313|EMBL:AEF39457.1};
GN   OrderedLocusNames=AS9A_1005 {ECO:0000313|EMBL:AEF39457.1};
OS   Hoyosella subflava (strain DSM 45089 / JCM 17490 / NBRC 109087 / DQS3-9A1)
OS   (Amycolicicoccus subflavus).
OC   Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Hoyosellaceae;
OC   Hoyosella.
OX   NCBI_TaxID=443218 {ECO:0000313|EMBL:AEF39457.1, ECO:0000313|Proteomes:UP000009235};
RN   [1] {ECO:0000313|EMBL:AEF39457.1, ECO:0000313|Proteomes:UP000009235}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 45089 / DQS3-9A1 {ECO:0000313|Proteomes:UP000009235};
RX   PubMed=21725023; DOI=10.1128/JB.05388-11;
RA   Cai M., Chen W.M., Nie Y., Chi C.Q., Wang Y.N., Tang Y.Q., Li G.Y.,
RA   Wu X.L.;
RT   "Complete genome sequence of Amycolicicoccus subflavus DQS3-9A1T, an
RT   actinomycete isolated from crude oil-polluted soil.";
RL   J. Bacteriol. 193:4538-4539(2011).
CC   -!- SIMILARITY: Belongs to the GcvT family.
CC       {ECO:0000256|ARBA:ARBA00008609}.
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DR   EMBL; CP002786; AEF39457.1; -; Genomic_DNA.
DR   AlphaFoldDB; F6EPM4; -.
DR   STRING; 443218.AS9A_1005; -.
DR   KEGG; asd:AS9A_1005; -.
DR   eggNOG; COG0404; Bacteria.
DR   eggNOG; COG0665; Bacteria.
DR   HOGENOM; CLU_007884_11_2_11; -.
DR   Proteomes; UP000009235; Chromosome.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   Gene3D; 2.40.30.110; Aminomethyltransferase beta-barrel domains; 1.
DR   Gene3D; 3.30.70.1400; Aminomethyltransferase beta-barrel domains; 1.
DR   Gene3D; 3.30.9.10; D-Amino Acid Oxidase, subunit A, domain 2; 1.
DR   Gene3D; 3.50.50.60; FAD/NAD(P)-binding domain; 1.
DR   InterPro; IPR006076; FAD-dep_OxRdtase.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR032503; FAO_M.
DR   InterPro; IPR028896; GCST/YgfZ/DmdA.
DR   InterPro; IPR013977; GCV_T_C.
DR   InterPro; IPR006222; GCV_T_N.
DR   InterPro; IPR029043; GcvT/YgfZ_C.
DR   InterPro; IPR027266; TrmE/GcvT_dom1.
DR   PANTHER; PTHR43757; AMINOMETHYLTRANSFERASE; 1.
DR   PANTHER; PTHR43757:SF2; AMINOMETHYLTRANSFERASE, MITOCHONDRIAL; 1.
DR   Pfam; PF01266; DAO; 1.
DR   Pfam; PF16350; FAO_M; 1.
DR   Pfam; PF01571; GCV_T; 1.
DR   Pfam; PF08669; GCV_T_C; 1.
DR   SUPFAM; SSF101790; Aminomethyltransferase beta-barrel domain; 1.
DR   SUPFAM; SSF54373; FAD-linked reductases, C-terminal domain; 1.
DR   SUPFAM; SSF51905; FAD/NAD(P)-binding domain; 1.
DR   SUPFAM; SSF103025; Folate-binding domain; 1.
PE   3: Inferred from homology;
KW   Reference proteome {ECO:0000313|Proteomes:UP000009235}.
FT   DOMAIN          2..367
FT                   /note="FAD dependent oxidoreductase"
FT                   /evidence="ECO:0000259|Pfam:PF01266"
FT   DOMAIN          370..425
FT                   /note="FAD dependent oxidoreductase central"
FT                   /evidence="ECO:0000259|Pfam:PF16350"
FT   DOMAIN          427..705
FT                   /note="Aminomethyltransferase folate-binding"
FT                   /evidence="ECO:0000259|Pfam:PF01571"
FT   DOMAIN          729..807
FT                   /note="Glycine cleavage T-protein C-terminal barrel"
FT                   /evidence="ECO:0000259|Pfam:PF08669"
SQ   SEQUENCE   815 AA;  88823 MW;  AC6A30A5E47B6934 CRC64;
     MIIGAGIVGA NLADELTVRG WDRVTVLDQG PLPLTGGSTS HAPGLVFQTN ASKALTEFAR
     YTVEKFKGLD VDGQWCFNQL GGLEVATNPE RLADLHRKQG WAASWGVADA AVLDPKECVK
     LHPLVDESQI LGGLYVPTDG LAKASRVVVA LIRRATQRGA EFRGSSKVTG IEHRAGQVTG
     VITPHGVIPA DIVVSCGGFW GPAIGEMVGM QVPLLPLAHQ YAKTGQVPEL VGRNSELAEA
     SMPILRHQDQ DLYFREHGDR LGIGSYAHRP MPVDLRDLTE SDEISVAAMP SMLPFTEDDF
     APSWEASKLL IPSLESTKVE EGFNGVFSFT PDGGPVVGES PEVAGFWIAE AVWVTHSAGV
     ARSVAQLLVD GRSEISLHGC DVHRFEEIQT SPDYVSETGQ QNFVEIYDVL HPLQPRTSPR
     NLRVSPFHAR QEELGAFFLE SGGWERPHWY EANTRLLDDL PSEWTSPPRD PWAAQFFSPI
     AAAEAWRTRT SVAMYDMTPL RRLEVSGPGA LALLTRLTTG KMDKSVGAVT YTLMLDGSGG
     IRSDLTVARL SETTFQLGAN GNIDVDYLRR EAPADGSVQI RDVTGGTCCI GVWGPRARDL
     VQALSADDFS GETFKYFRSK KVWIAGIPIT AMRLSYVGEL GWELYTSAEY GLRLWDALWG
     EGQQYDVIAA GRAAFNSLRI EKGYRAWGSD MTTEHNPYEA GLSFAVNANK QDYVGYDAIQ
     RIKQEPLERR LVCVAIDDGQ SVVLGHEPAF IDGTPVGYVT SAAFGHTVGT PLAYVWLPAS
     AAPGTNIEIQ YFDRRIAATV VAEPLVDPDM KRIKA
//