ID F6HWU3_VITVI Unreviewed; 502 AA.
AC F6HWU3;
DT 27-JUL-2011, integrated into UniProtKB/TrEMBL.
DT 27-JUL-2011, sequence version 1.
DT 27-MAR-2024, entry version 54.
DE RecName: Full=Splicing factor U2af large subunit {ECO:0000256|RuleBase:RU364135};
DE AltName: Full=U2 auxiliary factor 65 kDa subunit {ECO:0000256|RuleBase:RU364135};
DE AltName: Full=U2 small nuclear ribonucleoprotein auxiliary factor large subunit (U2 snRNP auxiliary factor large subunit) {ECO:0000256|RuleBase:RU364135};
GN ORFNames=VIT_00s0561g00030 {ECO:0000313|EMBL:CCB59157.1};
OS Vitis vinifera (Grape).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; Vitales; Vitaceae; Viteae; Vitis.
OX NCBI_TaxID=29760 {ECO:0000313|Proteomes:UP000009183};
RN [1] {ECO:0000313|Proteomes:UP000009183}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Pinot noir / PN40024 {ECO:0000313|Proteomes:UP000009183};
RX PubMed=17721507; DOI=10.1038/nature06148;
RG The French-Italian Public Consortium for Grapevine Genome Characterization.;
RA Jaillon O., Aury J.-M., Noel B., Policriti A., Clepet C., Casagrande A.,
RA Choisne N., Aubourg S., Vitulo N., Jubin C., Vezzi A., Legeai F.,
RA Hugueney P., Dasilva C., Horner D., Mica E., Jublot D., Poulain J.,
RA Bruyere C., Billault A., Segurens B., Gouyvenoux M., Ugarte E.,
RA Cattonaro F., Anthouard V., Vico V., Del Fabbro C., Alaux M.,
RA Di Gaspero G., Dumas V., Felice N., Paillard S., Juman I., Moroldo M.,
RA Scalabrin S., Canaguier A., Le Clainche I., Malacrida G., Durand E.,
RA Pesole G., Laucou V., Chatelet P., Merdinoglu D., Delledonne M.,
RA Pezzotti M., Lecharny A., Scarpelli C., Artiguenave F., Pe M.E., Valle G.,
RA Morgante M., Caboche M., Adam-Blondon A.-F., Weissenbach J., Quetier F.,
RA Wincker P.;
RT "The grapevine genome sequence suggests ancestral hexaploidization in major
RT angiosperm phyla.";
RL Nature 449:463-467(2007).
CC -!- FUNCTION: Necessary for the splicing of pre-mRNA.
CC {ECO:0000256|RuleBase:RU364135}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123,
CC ECO:0000256|RuleBase:RU364135}.
CC -!- SIMILARITY: Belongs to the splicing factor SR family.
CC {ECO:0000256|ARBA:ARBA00010269, ECO:0000256|RuleBase:RU364135}.
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DR EMBL; FN596301; CCB59157.1; -; Genomic_DNA.
DR AlphaFoldDB; F6HWU3; -.
DR STRING; 29760.F6HWU3; -.
DR PaxDb; 29760-VIT_00s0561g00030-t01; -.
DR eggNOG; KOG0120; Eukaryota.
DR HOGENOM; CLU_021795_1_1_1; -.
DR InParanoid; F6HWU3; -.
DR Proteomes; UP000009183; Unassembled WGS sequence, unordered.
DR GO; GO:0000243; C:commitment complex; IBA:GO_Central.
DR GO; GO:0016607; C:nuclear speck; IBA:GO_Central.
DR GO; GO:0071004; C:U2-type prespliceosome; IBA:GO_Central.
DR GO; GO:0089701; C:U2AF complex; IBA:GO_Central.
DR GO; GO:0008187; F:poly-pyrimidine tract binding; IBA:GO_Central.
DR GO; GO:0030628; F:pre-mRNA 3'-splice site binding; IBA:GO_Central.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR GO; GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
DR CDD; cd12230; RRM1_U2AF65; 1.
DR CDD; cd12231; RRM2_U2AF65; 1.
DR CDD; cd12232; RRM3_U2AF65; 1.
DR Gene3D; 3.30.70.330; -; 3.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR InterPro; IPR006529; U2AF_lg.
DR NCBIfam; TIGR01642; U2AF_lg; 1.
DR PANTHER; PTHR23139; RNA-BINDING PROTEIN; 1.
DR PANTHER; PTHR23139:SF9; SPLICING FACTOR U2AF 65 KDA SUBUNIT; 1.
DR Pfam; PF00076; RRM_1; 1.
DR SMART; SM00360; RRM; 2.
DR SUPFAM; SSF54928; RNA-binding domain, RBD; 2.
DR PROSITE; PS50102; RRM; 2.
PE 3: Inferred from homology;
KW mRNA processing {ECO:0000256|ARBA:ARBA00022664,
KW ECO:0000256|RuleBase:RU364135};
KW mRNA splicing {ECO:0000256|ARBA:ARBA00023187,
KW ECO:0000256|RuleBase:RU364135};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242, ECO:0000256|RuleBase:RU364135};
KW Reference proteome {ECO:0000313|Proteomes:UP000009183};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884, ECO:0000256|PROSITE-
KW ProRule:PRU00176}.
FT DOMAIN 149..233
FT /note="RRM"
FT /evidence="ECO:0000259|PROSITE:PS50102"
FT DOMAIN 273..351
FT /note="RRM"
FT /evidence="ECO:0000259|PROSITE:PS50102"
SQ SEQUENCE 502 AA; 54148 MW; 079C957376B54B7F CRC64;
MIIVVIERGD RDRDHVPVQR IDLETDLNHA LVPVLAQRAN GPVVLTWHHL SLHCCQVPLS
QAMLGYMSHC ATHGRGHRRY YVQPGTNAPI SWILFAFNRQ LLPFGELPGV PQMVPGMIQN
MFPFGATQLG ALPLMPVQAM TQQATRHARR VYVGGLPPLA NEQTIATFFS QVMTAIGGNS
AGPGDAVVNV YINHEKKFAF VEMRSVEEAS NAMALDGIMF EACLTLIFSL VRRPTDYNPS
LAAALGPSQP SPHLNLAAVG LMPGVIGGAE GPDRIFVGGL PYYFTEEQIR ELLESFGPLR
GFDLVKDRDT GNSKGYGFCV YQDPAVTDIA CAALNGLKMG DKTLTVRRAT VGSGQAKSEQ
DNILAQAQQH IAIQKIALQA GGLNLPGAGM AFTAIAETPT KVLCLTEVSS VPKIIYHILY
KWIASGILFL LLVHGALVHV VIPRPSPNGD LIPGVGKVFL EYSDTAGSSS ARNALSGRKF
GGNVVSAVYY PEDKYYDGDY GA
//
