ID F6IKA1_9SPHN Unreviewed; 601 AA.
AC F6IKA1;
DT 27-JUL-2011, integrated into UniProtKB/TrEMBL.
DT 27-JUL-2011, sequence version 1.
DT 27-MAR-2024, entry version 62.
DE SubName: Full=Isoamylase {ECO:0000313|EMBL:CCA91919.1};
DE EC=3.2.1.68 {ECO:0000313|EMBL:CCA91919.1};
GN ORFNames=PP1Y_AT9845 {ECO:0000313|EMBL:CCA91919.1};
OS Novosphingobium sp. PP1Y.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
OC Sphingomonadaceae; Novosphingobium.
OX NCBI_TaxID=702113 {ECO:0000313|EMBL:CCA91919.1, ECO:0000313|Proteomes:UP000009242};
RN [1] {ECO:0000313|EMBL:CCA91919.1, ECO:0000313|Proteomes:UP000009242}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=sp. PP1Y {ECO:0000313|Proteomes:UP000009242};
RX PubMed=21685292; DOI=10.1128/JB.05349-11;
RA D'Argenio V., Petrillo M., Cantiello P., Naso B., Cozzuto L., Notomista E.,
RA Paolella G., Di Donato A., Salvatore F.;
RT "De novo sequencing and assembly of the whole genome of Novosphingobium sp.
RT strain PP1Y.";
RL J. Bacteriol. 193:4296-4296(2011).
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family.
CC {ECO:0000256|ARBA:ARBA00008061}.
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DR EMBL; FR856862; CCA91919.1; -; Genomic_DNA.
DR RefSeq; WP_013832320.1; NC_015580.1.
DR AlphaFoldDB; F6IKA1; -.
DR KEGG; npp:PP1Y_AT9845; -.
DR eggNOG; COG1523; Bacteria.
DR HOGENOM; CLU_011725_1_1_5; -.
DR OrthoDB; 3236218at2; -.
DR Proteomes; UP000009242; Chromosome.
DR GO; GO:0004133; F:glycogen debranching enzyme activity; IEA:InterPro.
DR GO; GO:0019156; F:isoamylase activity; IEA:UniProtKB-EC.
DR GO; GO:0005980; P:glycogen catabolic process; IEA:InterPro.
DR CDD; cd11326; AmyAc_Glg_debranch; 1.
DR CDD; cd02856; E_set_GDE_Isoamylase_N; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR InterPro; IPR044505; GlgX_Isoamylase_N_E_set.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR004193; Glyco_hydro_13_N.
DR InterPro; IPR011837; Glycogen_debranch_GlgX.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR NCBIfam; TIGR02100; glgX_debranch; 1.
DR PANTHER; PTHR43002; GLYCOGEN DEBRANCHING ENZYME; 1.
DR PANTHER; PTHR43002:SF1; ISOAMYLASE 1, CHLOROPLASTIC; 1.
DR Pfam; PF02922; CBM_48; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF81296; E set domains; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000313|EMBL:CCA91919.1};
KW Hydrolase {ECO:0000313|EMBL:CCA91919.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000009242}.
FT DOMAIN 138..537
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|SMART:SM00642"
FT REGION 438..464
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 601 AA; 66633 MW; 8F23CB18BADCCC6A CRC64;
MIPLGARVRE GTTRFAVRSP RAERVWLCLF TGRSETRHEM ARDGEVWTLE LPEDLSGARY
GYRAAGKWAP EQGCWFDPAK LLVDPYAVEL DRRFRQDPRL AAFGKDTAAI VPKARVPASQ
PSVPVETPRF AAGGLVYEIN VRAFTMRHPD VPRRLRGTVA ALAHPAVVAH LKKLHVTAVE
LMPVVAWIDE RHLPPLGLVN AWGYNPVAMM ALDPGLCPGG VAELRKTVAA LHAEGIGVLL
DLVFNHTGES DVEGGVISMR GLDDAVYGRA PDGALINDSG CGNTLDFANP AVRRIALDAM
RHFVSQCGVD GFRFDLAPIL ARGPGFDPAA PIFAEIEDDP LLSSRVLIAE PWDIGPGGYQ
LANFPAHWLE WNDRFRDDVR RFWREEEGVG KLATRLAGSS DIFGPHWPHA DCRSVNFLAA
HDGFTLADTV SYAHRHNEAN GEENRDGHGE NYSWNNGAEG PTDDPHVLAR RAHDLRALLG
TLFASTGTIM LTAGDEFGRT QQGNNNAYCQ DNEIGWIDWE SRDLALEDHV ARLSLVRAKR
RAWFARFPDT VAWHRLDGEP KSVADWENPH SEGFVYEARE QDGGIRVEVR RHDREVRLAA
I
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