ID F6MHK8_9ASTR Unreviewed; 1385 AA.
AC F6MHK8;
DT 27-JUL-2011, integrated into UniProtKB/TrEMBL.
DT 27-JUL-2011, sequence version 1.
DT 27-MAR-2024, entry version 38.
DE RecName: Full=DNA-directed RNA polymerase subunit beta'' {ECO:0000256|HAMAP-Rule:MF_01324};
DE EC=2.7.7.6 {ECO:0000256|HAMAP-Rule:MF_01324};
DE AltName: Full=PEP {ECO:0000256|HAMAP-Rule:MF_01324};
DE AltName: Full=Plastid-encoded RNA polymerase subunit beta'' {ECO:0000256|HAMAP-Rule:MF_01324};
DE Short=RNA polymerase subunit beta'' {ECO:0000256|HAMAP-Rule:MF_01324};
GN Name=rpoC2 {ECO:0000256|HAMAP-Rule:MF_01324,
GN ECO:0000313|EMBL:AEG64545.1};
OS Ageratina adenophora.
OG Plastid; Chloroplast {ECO:0000313|EMBL:AEG64545.1}.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; campanulids; Asterales; Asteraceae; Asteroideae;
OC Heliantheae alliance; Eupatorieae; Ageratina.
OX NCBI_TaxID=176616 {ECO:0000313|EMBL:AEG64545.1};
RN [1] {ECO:0000313|EMBL:AEG64545.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=22606302; DOI=10.1371/journal.pone.0036869;
RA Nie X., Lv S., Zhang Y., Du X., Wang L., Biradar S.S., Tan X., Wan F.,
RA Weining S.;
RT "Complete Chloroplast Genome Sequence of a Major Invasive Species, Crofton
RT Weed (Ageratina adenophora).";
RL PLoS ONE 7:E36869-E36869(2012).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000256|HAMAP-Rule:MF_01324}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000256|HAMAP-Rule:MF_01324};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_01324};
CC Note=Binds 1 Zn(2+) ion per subunit. {ECO:0000256|HAMAP-Rule:MF_01324};
CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC composed of four subunits: alpha, beta, beta', and beta''. When a
CC (nuclear-encoded) sigma factor is associated with the core the
CC holoenzyme is formed, which can initiate transcription.
CC {ECO:0000256|HAMAP-Rule:MF_01324}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000256|HAMAP-
CC Rule:MF_01324}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC2
CC subfamily. {ECO:0000256|HAMAP-Rule:MF_01324}.
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DR EMBL; JF826503; AEG64545.1; -; Genomic_DNA.
DR RefSeq; YP_004564361.1; NC_015621.1.
DR GeneID; 10744213; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006351; P:DNA-templated transcription; IEA:UniProtKB-UniRule.
DR CDD; cd02655; RNAP_beta'_C; 1.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.150.390; -; 1.
DR Gene3D; 1.10.1790.20; -; 1.
DR Gene3D; 1.10.274.100; RNA polymerase Rpb1, domain 3; 1.
DR HAMAP; MF_01324; RNApol_bact_RpoC2; 1.
DR InterPro; IPR012756; DNA-dir_RpoC2_beta_pp.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR NCBIfam; TIGR02388; rpoC2_cyan; 1.
DR PANTHER; PTHR48355; DNA-DIRECTED RNA POLYMERASE SUBUNIT BETA; 1.
DR PANTHER; PTHR48355:SF1; DNA-DIRECTED RNA POLYMERASE SUBUNIT BETA; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 2.
DR SUPFAM; SSF64484; beta and beta-prime subunits of DNA dependent RNA-polymerase; 1.
PE 3: Inferred from homology;
KW Chloroplast {ECO:0000313|EMBL:AEG64545.1};
KW DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022478,
KW ECO:0000256|HAMAP-Rule:MF_01324};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723, ECO:0000256|HAMAP-
KW Rule:MF_01324};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695, ECO:0000256|HAMAP-
KW Rule:MF_01324}; Plastid {ECO:0000313|EMBL:AEG64545.1};
KW Transcription {ECO:0000256|ARBA:ARBA00023163, ECO:0000256|HAMAP-
KW Rule:MF_01324};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|HAMAP-
KW Rule:MF_01324};
KW Zinc {ECO:0000256|ARBA:ARBA00022833, ECO:0000256|HAMAP-Rule:MF_01324}.
FT DOMAIN 97..161
FT /note="RNA polymerase Rpb1"
FT /evidence="ECO:0000259|Pfam:PF05000"
FT DOMAIN 176..369
FT /note="RNA polymerase Rpb1"
FT /evidence="ECO:0000259|Pfam:PF04998"
FT DOMAIN 1194..1276
FT /note="RNA polymerase Rpb1"
FT /evidence="ECO:0000259|Pfam:PF04998"
FT BINDING 224
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01324"
FT BINDING 295
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01324"
FT BINDING 302
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01324"
FT BINDING 305
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01324"
SQ SEQUENCE 1385 AA; 157223 MW; 16B83A6A52E5DC8F CRC64;
MEVLMAERPT QVFHNKVIDG TAMKRLISRF IDHYGIGYTS HILDQVKTLG FRQATAASIS
LGIDDLLTIP SKRWLVQDAE QQSFILEKHH HYGNVHAVEK LRQSIEIWYA TSEYLRQEMT
PNFRMTDPFN PVHIMSFSGA RGNASQVHQL VGMRGLMSDP QGQMIDLPIQ SNLREGLSLT
EYIISCYGAR KGVVDTAIRT SDAGYLTRRL VEVVQHIVVR RTDCGTVRGI SVSPRNGMMA
DRIFIQTLIG RVLADDIYIG SRCIATRNQD IGVGLVNRFI TFRAQPISIR TPFTCRSTSW
ICQLCYGRSP AHDDLVELGE AVGIIAGQSI GEPGTQLTLR TFHTGGVFTG GTAEHVRAPS
NGKIKFNEDL VHPTRTRHGH PAFLCSRDLY VTIESEDIIH NVCIPPKSFL LVQNDQYVES
EQVIAEIRAR TSTLNLKEKV RKHIYSDSEG EMHWNTDVYH APEFTYGNIH LLPKTSHLWI
LLGEPWRSSL GPCSIHKDQD QMNAYSLSVK RRYISNPSVT NNEVRNKFFS SYFSGKNQKG
DRIPDYSELN RMTCTGRCNL RYRGILDGNS DLLAKRRRNR VIIPLDSIQE GENQLIPSSG
ISIEIPRNGI LRRNSILAYF DDPRYIRKSS GLTKYETREL NSIVNEEDLI EYRGVKVFWP
KYQKEVNPFF FIPVEVHVLA ESSSIMVRHN SIIGVDTEIT LNRRSRVGGL VRVKKKAEKM
KLIIFSGDIH FPGKTNKAFR LIPPGGGKQN SKKYKKLKNW LYIQRMKLSR YEKKYFVLVQ
PVVPYKKTDG INLGRLFPAD LLQESDNLQL RVVNYILYYD PILEFWDTSI QLVRTCLVLN
WDQDKKIEKA CASFVEISTN GLIRDFLRID LAKSPISYTG KRNNLPGSGL ISENGSDRAN
VNPFSSIYSY SKARIQESLN PNQGTIHTLL NRNKESQSLI ILSSSNCSRI GPFNDVKSPN
VIKESIKKKD PLISIISIRN SLGPLGTGFP IYNFDLFSHL ITHNQILVTN YLQLDNLKQI
FQILKYYLLD ENGKIYNPYS CSNIILNPFK LNWYFLHYNY CEETSTIVSL GQFLCEKVCI
AKKGPHLKSG QVLIIQVDSV VIRSAKPYLA TPGATVHGHY GKILYDGDTL VTFIYEKSRS
GDITQGLPKV EQVLEVRSID SISMNLEKRI EGWNKCITRI IGIPWAFFIG AELTIMQSRI
SLVNKVQKVY RSQGVQIHNR HIEIIVRQIT SKVLVSEDEM SNVFSPGELI GLLRAERMGR
ALEEAICYQA VLLGITRASM NTQSFISEAS FQETARVLAK AALLGRIDWL KGLKENVVLG
GMIPVGSGFK TPSSEPNNIP NNIAFELKKK NLLEGEMKDI LFYHRKLFDS CLSKNFHDTQ
EQSFF
//
