ID F7AJR0_HORSE Unreviewed; 936 AA.
AC F7AJR0;
DT 27-JUL-2011, integrated into UniProtKB/TrEMBL.
DT 11-DEC-2019, sequence version 3.
DT 27-MAR-2024, entry version 64.
DE RecName: Full=RING-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012483};
DE EC=2.3.2.27 {ECO:0000256|ARBA:ARBA00012483};
DE AltName: Full=Zinc/RING finger protein 3 {ECO:0000256|ARBA:ARBA00029838};
GN Name=ZNRF3 {ECO:0000313|Ensembl:ENSECAP00000009563.3,
GN ECO:0000313|VGNC:VGNC:25402};
OS Equus caballus (Horse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Perissodactyla; Equidae; Equus.
OX NCBI_TaxID=9796 {ECO:0000313|Ensembl:ENSECAP00000009563.3, ECO:0000313|Proteomes:UP000002281};
RN [1] {ECO:0000313|Ensembl:ENSECAP00000009563.3, ECO:0000313|Proteomes:UP000002281}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Thoroughbred {ECO:0000313|Ensembl:ENSECAP00000009563.3,
RC ECO:0000313|Proteomes:UP000002281};
RX PubMed=19892987; DOI=10.1126/science.1178158;
RG Broad Institute Genome Sequencing Platform;
RG Broad Institute Whole Genome Assembly Team;
RA Wade C.M., Giulotto E., Sigurdsson S., Zoli M., Gnerre S., Imsland F.,
RA Lear T.L., Adelson D.L., Bailey E., Bellone R.R., Bloecker H., Distl O.,
RA Edgar R.C., Garber M., Leeb T., Mauceli E., MacLeod J.N., Penedo M.C.T.,
RA Raison J.M., Sharpe T., Vogel J., Andersson L., Antczak D.F., Biagi T.,
RA Binns M.M., Chowdhary B.P., Coleman S.J., Della Valle G., Fryc S.,
RA Guerin G., Hasegawa T., Hill E.W., Jurka J., Kiialainen A., Lindgren G.,
RA Liu J., Magnani E., Mickelson J.R., Murray J., Nergadze S.G., Onofrio R.,
RA Pedroni S., Piras M.F., Raudsepp T., Rocchi M., Roeed K.H., Ryder O.A.,
RA Searle S., Skow L., Swinburne J.E., Syvaenen A.C., Tozaki T., Valberg S.J.,
RA Vaudin M., White J.R., Zody M.C., Lander E.S., Lindblad-Toh K.;
RT "Genome sequence, comparative analysis, and population genetics of the
RT domestic horse.";
RL Science 326:865-867(2009).
RN [2] {ECO:0000313|Ensembl:ENSECAP00000009563.3}
RP IDENTIFICATION.
RC STRAIN=Thoroughbred {ECO:0000313|Ensembl:ENSECAP00000009563.3};
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27; Evidence={ECO:0000256|ARBA:ARBA00000900};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000256|ARBA:ARBA00004906}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004251};
CC Single-pass type I membrane protein {ECO:0000256|ARBA:ARBA00004251}.
CC Membrane {ECO:0000256|ARBA:ARBA00004479}; Single-pass type I membrane
CC protein {ECO:0000256|ARBA:ARBA00004479}.
CC -!- SIMILARITY: Belongs to the ZNRF3 family.
CC {ECO:0000256|ARBA:ARBA00008759}.
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DR AlphaFoldDB; F7AJR0; -.
DR SMR; F7AJR0; -.
DR STRING; 9796.ENSECAP00000009563; -.
DR PaxDb; 9796-ENSECAP00000009563; -.
DR Ensembl; ENSECAT00000012138.4; ENSECAP00000009563.3; ENSECAG00000011694.4.
DR VGNC; VGNC:25402; ZNRF3.
DR GeneTree; ENSGT00940000154006; -.
DR HOGENOM; CLU_018099_1_0_1; -.
DR InParanoid; F7AJR0; -.
DR OMA; LCEGCGP; -.
DR TreeFam; TF317074; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000002281; Chromosome 8.
DR Bgee; ENSECAG00000011694; Expressed in trophectoderm and 23 other cell types or tissues.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005109; F:frizzled binding; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IBA:GO_Central.
DR GO; GO:0090090; P:negative regulation of canonical Wnt signaling pathway; IBA:GO_Central.
DR GO; GO:2000051; P:negative regulation of non-canonical Wnt signaling pathway; IEA:Ensembl.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR GO; GO:2000095; P:regulation of Wnt signaling pathway, planar cell polarity pathway; IEA:Ensembl.
DR GO; GO:0072089; P:stem cell proliferation; IEA:Ensembl.
DR GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR GO; GO:0038018; P:Wnt receptor catabolic process; IBA:GO_Central.
DR CDD; cd16799; RING-H2_ZNRF3; 1.
DR Gene3D; 3.50.30.30; -; 1.
DR Gene3D; 3.30.40.10; Zinc/RING finger domain, C3HC4 (zinc finger); 1.
DR InterPro; IPR001841; Znf_RING.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR InterPro; IPR040700; ZNRF-3_ecto.
DR InterPro; IPR045903; ZNRF3_Znf_RING.
DR PANTHER; PTHR16200:SF3; E3 UBIQUITIN-PROTEIN LIGASE ZNRF3; 1.
DR PANTHER; PTHR16200; RING ZINC FINGER; 1.
DR Pfam; PF13639; zf-RING_2; 1.
DR Pfam; PF18212; ZNRF_3_ecto; 1.
DR SMART; SM00184; RING; 1.
DR SUPFAM; SSF57850; RING/U-box; 1.
DR PROSITE; PS50089; ZF_RING_2; 1.
PE 3: Inferred from homology;
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000002281};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}; Zinc {ECO:0000256|ARBA:ARBA00022833};
KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771, ECO:0000256|PROSITE-
KW ProRule:PRU00175}.
FT TRANSMEM 214..236
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 291..332
FT /note="RING-type"
FT /evidence="ECO:0000259|PROSITE:PS50089"
FT REGION 1..32
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 604..654
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 668..692
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 902..936
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 11..27
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 668..686
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 903..921
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 936 AA; 100506 MW; 574F5E2DF3F53E4E CRC64;
MRPRSGGRPG APGCRRRRLR RRPGRGRRLP PPPPLPLLLG LLLAAAGPGA ARAKETAFVE
VVLFESSPSG DYTTYTTGLT GRFSRAGATL SAEGEIVQMH PLGLCNNNDE EDLYEYGWVG
VVKLEQPELD PKPCLTVLGK AKRAVQRGAT AVIFDVSENP EAIDQLNQGS EDPLKRPVVY
VKGADAIKLM NIVNKQKVAR ARIQHRPPRQ PTEYFDMGIF LAFFVVVSLV CLILLVKIKL
KQRRSQNSMN RLAVQALEKM ETRKFNSKSK GRREGSCGAL DTLSSSSTSD CAICLEKYID
GEELRVIPCT HRFHRKCVDP WLLQHHTCPH CRHNIIEQKG NPSTMCVETS NLARSRQQRV
ILPVHYPGRV HRTNAIPAYP TRTSMDSHGN PVTLLTMDRH GEQSLYSPQT PAYIRGYPPL
HLDHTLAPHR CGLEHRAYSP AHPFRRPKFS GRSFSKAACF SQYETMYQHY YFQGLSYPEQ
EGQAAPSLAP RGPSRAFPPS GSSSLLFPTV VHMAPPSHLE SGSTSSFSCY HGHRSVCSGY
LADCPGSDSS SSSSSSGQCH CSSSDSVVDC TEVSNQGVYG SCSTFRSSLS SDYDPFIYRS
RSPCRTSDVG GSGTSGWGPV MRLEGSLPPE ELLTAHSPGA GRGEPWPGPA SPVGDRLSTC
SLEMDYSSNS SLEHRAPNSS TSEVGLEASP GAAPDLRRTW KGSHEGPSCA CCCELQPSAL
EPGVGAAGGS TLFLGTPLCE GCGPTGGESQ PGSSQGLYGL HPDHLPRTDG VKYEGLPCCF
YEEKQVAHGG GGGSGCYTED CSVSVQYTLA QEALPSCHPG ARDLSQRIPI IPEDVDCELG
LSLDCHGTHG LGPWGGTPGL DALRPHRGLG AAQEEERVLC CQARAPLPPG CLLEEAGATR
ANFPGASQDT QESNATATEA AGQRSHPAEG GGSPGA
//
