ID F7QWU5_9LACO Unreviewed; 463 AA.
AC F7QWU5;
DT 21-SEP-2011, integrated into UniProtKB/TrEMBL.
DT 21-SEP-2011, sequence version 1.
DT 27-MAR-2024, entry version 29.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN ORFNames=LSGJ_01794 {ECO:0000313|EMBL:EGM49732.1};
OS Ligilactobacillus salivarius GJ-24.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae;
OC Ligilactobacillus.
OX NCBI_TaxID=1041521 {ECO:0000313|EMBL:EGM49732.1, ECO:0000313|Proteomes:UP000003074};
RN [1] {ECO:0000313|EMBL:EGM49732.1, ECO:0000313|Proteomes:UP000003074}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=GJ-24 {ECO:0000313|EMBL:EGM49732.1,
RC ECO:0000313|Proteomes:UP000003074};
RX PubMed=21742893; DOI=10.1128/JB.05616-11;
RA Cho Y.J., Choi J.K., Kim J.H., Lim Y.S., Ham J.S., Kang D.K., Chun J.,
RA Paik H.D., Kim G.B.;
RT "Genome Sequence of Lactobacillus salivarius GJ-24, a Probiotic Strain
RT Isolated from Healthy Adult Intestine.";
RL J. Bacteriol. 193:5021-5022(2011).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EGM49732.1}.
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DR EMBL; AFOI01000009; EGM49732.1; -; Genomic_DNA.
DR RefSeq; WP_003708679.1; NZ_AFOI01000009.1.
DR AlphaFoldDB; F7QWU5; -.
DR REBASE; 39828; M.LsaGJ24ORF1794P.
DR PATRIC; fig|1041521.3.peg.1809; -.
DR Proteomes; UP000003074; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0006306; P:DNA methylation; IEA:InterPro.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR003356; DNA_methylase_A-5.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR42933:SF1; SITE-SPECIFIC DNA-METHYLTRANSFERASE (ADENINE-SPECIFIC); 1.
DR PANTHER; PTHR42933; SLR6095 PROTEIN; 1.
DR Pfam; PF02384; N6_Mtase; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
PE 4: Predicted;
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 153..424
FT /note="DNA methylase adenine-specific"
FT /evidence="ECO:0000259|Pfam:PF02384"
FT COILED 425..455
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 463 AA; 53316 MW; 10FB0AB43743675A CRC64;
MDKVLEERLA AKDSYIDIHD FYVLMAFYQS ISQKVLRKFD KDGSILEVQK KYEEGKEMVQ
DAQFVIAPQD TYYHHYQMYK ANKFEYVELV KSLYNIEEKN PKLKNIFQDV RGSTSSLPSE
TAFEKIDSRN EVSFEENLEI IKRSSGAKDN YDYTSKNIRK LITKLVGSKK EGVSIYDPAL
GTASLLLGIN QAALKENRYY GQDISTQAIK TAIMNAIVND IAEDKFEFKN ENTLANNWEF
GKVDIVVSDP PINMKWNVDR NLSQDRRYRD YGEMPNKADW GFILDGIDKL SDNGMMVVSV
VQGTLFRGAK EYNIRRKLLE DGKVRAVIQL PGNTKLSTTI ATCLLVIRKS SEDKDVFFIN
ASQEYEKKGL ENILTEANVD KIVDTFNEKK GEQGFSHMAS YEEIEKNDFN LSVARYVNQY
KFKEKLDYQE EIKNLEKLDE KLSQTDATLK NLMGDLGLME IDN
//
