ID F7R0F2_9LACO Unreviewed; 335 AA.
AC F7R0F2;
DT 21-SEP-2011, integrated into UniProtKB/TrEMBL.
DT 21-SEP-2011, sequence version 1.
DT 27-MAR-2024, entry version 32.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN ORFNames=LRU_01281 {ECO:0000313|EMBL:EGM51767.1};
OS Ligilactobacillus ruminis SPM0211.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae;
OC Ligilactobacillus.
OX NCBI_TaxID=1040964 {ECO:0000313|EMBL:EGM51767.1, ECO:0000313|Proteomes:UP000002971};
RN [1] {ECO:0000313|EMBL:EGM51767.1, ECO:0000313|Proteomes:UP000002971}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SPM0211 {ECO:0000313|EMBL:EGM51767.1,
RC ECO:0000313|Proteomes:UP000002971};
RX PubMed=21742873; DOI=10.1128/JB.05601-11;
RA Lee S., Cho Y.J., Lee A.H., Chun J., Ha N.J., Ko G.;
RT "Genome Sequence of Lactobacillus ruminis SPM0211, Isolated from a Fecal
RT Sample from a Healthy Korean.";
RL J. Bacteriol. 193:5034-5034(2011).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EGM51767.1}.
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DR EMBL; AFOJ01000005; EGM51767.1; -; Genomic_DNA.
DR RefSeq; WP_003695480.1; NZ_AFOJ01000005.1.
DR AlphaFoldDB; F7R0F2; -.
DR Proteomes; UP000002971; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0006306; P:DNA methylation; IEA:InterPro.
DR CDD; cd02440; AdoMet_MTases; 1.
DR Gene3D; 1.10.150.470; -; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR003356; DNA_methylase_A-5.
DR InterPro; IPR016843; S-AdoMet-dep_Ade-MeTrfase_prd.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR048375; YtxK-like_N.
DR PANTHER; PTHR42933; SLR6095 PROTEIN; 1.
DR PANTHER; PTHR42933:SF3; TYPE I RESTRICTION ENZYME MJAVIII METHYLASE SUBUNIT; 1.
DR Pfam; PF02384; N6_Mtase; 1.
DR Pfam; PF21106; YtxK_like; 1.
DR PIRSF; PIRSF026567; Adenine_mtase_bact_prd; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
PE 4: Predicted;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603,
KW ECO:0000313|EMBL:EGM51767.1};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:EGM51767.1}.
FT DOMAIN 8..86
FT /note="YtxK-like N-terminal helical"
FT /evidence="ECO:0000259|Pfam:PF21106"
FT DOMAIN 98..307
FT /note="DNA methylase adenine-specific"
FT /evidence="ECO:0000259|Pfam:PF02384"
SQ SEQUENCE 335 AA; 37697 MW; 2F5F40277E112055 CRC64;
MALADIEKKY DLMNEACELL QEKLQDDCLD ALIENFDNLL NDGKVHVEDG IPDEKTASKL
AEIYRAVRLD EISTEDRRLL LQLSLLTVYR KEKIQANHQM TPDSIGFLTA YLLQQVYEKK
DETNFLDLCV GTGNLAAVVI NALKNNGFKN IHGFGIDNDD TLLTIASIES QLCDLDLNLY
HQDALDKSLI PQADVIVSDL PVGWYPLDER AEGYETHAKE GHSFVHFLLI EQALDNLKEG
GIGMFLIPSS MFESEESLPL LKFIQKNGYL QSLINLPGAM FASKKSEKAI LILQKKGAKS
KQANPVLLGE FPLVKDTKNF EKFMAEVAVW KEKNF
//
