ID   F8GY90_CUPNN            Unreviewed;       563 AA.
AC   F8GY90;
DT   21-SEP-2011, integrated into UniProtKB/TrEMBL.
DT   21-SEP-2011, sequence version 1.
DT   27-MAR-2024, entry version 54.
DE   SubName: Full=Choline dehydrogenase BetA {ECO:0000313|EMBL:AEI82831.1};
DE            EC=1.1.99.1 {ECO:0000313|EMBL:AEI82831.1};
GN   Name=betA {ECO:0000313|EMBL:AEI82831.1};
GN   OrderedLocusNames=CNE_BB2p00120 {ECO:0000313|EMBL:AEI82831.1};
OS   Cupriavidus necator (strain ATCC 43291 / DSM 13513 / CCUG 52238 / LMG 8453
OS   / N-1) (Ralstonia eutropha).
OG   Plasmid pBB2 {ECO:0000313|EMBL:AEI82831.1,
OG   ECO:0000313|Proteomes:UP000006798}.
OC   Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Cupriavidus.
OX   NCBI_TaxID=1042878 {ECO:0000313|EMBL:AEI82831.1, ECO:0000313|Proteomes:UP000006798};
RN   [1] {ECO:0000313|EMBL:AEI82831.1, ECO:0000313|Proteomes:UP000006798}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43291 / DSM 13513 / CCUG 52238 / LMG 8453 / N-1
RC   {ECO:0000313|Proteomes:UP000006798};
RC   PLASMID=pBB2 {ECO:0000313|EMBL:AEI82831.1,
RC   ECO:0000313|Proteomes:UP000006798};
RX   PubMed=21742890; DOI=10.1128/JB.05660-11;
RA   Poehlein A., Kusian B., Friedrich B., Daniel R., Bowien B.;
RT   "Complete genome sequence of the type strain Cupriavidus necator N-1.";
RL   J. Bacteriol. 193:5017-5017(2011).
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC         Evidence={ECO:0000256|ARBA:ARBA00001974,
CC         ECO:0000256|PIRSR:PIRSR000137-2};
CC   -!- SIMILARITY: Belongs to the GMC oxidoreductase family.
CC       {ECO:0000256|ARBA:ARBA00010790}.
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DR   EMBL; CP002880; AEI82831.1; -; Genomic_DNA.
DR   RefSeq; WP_013954114.1; NC_015724.1.
DR   AlphaFoldDB; F8GY90; -.
DR   KEGG; cnc:CNE_BB2p00120; -.
DR   HOGENOM; CLU_002865_7_1_4; -.
DR   Proteomes; UP000006798; Plasmid pBB2.
DR   GO; GO:0008812; F:choline dehydrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
DR   Gene3D; 3.50.50.60; FAD/NAD(P)-binding domain; 2.
DR   Gene3D; 3.30.560.10; Glucose Oxidase, domain 3; 1.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR012132; GMC_OxRdtase.
DR   InterPro; IPR000172; GMC_OxRdtase_N.
DR   InterPro; IPR007867; GMC_OxRtase_C.
DR   PANTHER; PTHR11552:SF147; CHOLINE DEHYDROGENASE, MITOCHONDRIAL; 1.
DR   PANTHER; PTHR11552; GLUCOSE-METHANOL-CHOLINE GMC OXIDOREDUCTASE; 1.
DR   Pfam; PF05199; GMC_oxred_C; 1.
DR   Pfam; PF00732; GMC_oxred_N; 1.
DR   PIRSF; PIRSF000137; Alcohol_oxidase; 2.
DR   SUPFAM; SSF54373; FAD-linked reductases, C-terminal domain; 1.
DR   SUPFAM; SSF51905; FAD/NAD(P)-binding domain; 1.
PE   3: Inferred from homology;
KW   FAD {ECO:0000256|ARBA:ARBA00022827, ECO:0000256|PIRSR:PIRSR000137-2};
KW   Flavoprotein {ECO:0000256|ARBA:ARBA00022630};
KW   Oxidoreductase {ECO:0000313|EMBL:AEI82831.1};
KW   Plasmid {ECO:0000313|EMBL:AEI82831.1}.
FT   DOMAIN          4..304
FT                   /note="Glucose-methanol-choline oxidoreductase N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF00732"
FT   DOMAIN          371..557
FT                   /note="Glucose-methanol-choline oxidoreductase C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF05199"
FT   BINDING         90
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR000137-2"
FT   BINDING         224
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR000137-2"
SQ   SEQUENCE   563 AA;  60324 MW;  340FE0848748A3AC CRC64;
     MTFYDDLIVG AGAAGSVLAN RLSADPQRRV LLLEAGRDIR PGDEPADIRS IFPLSTFNPA
     YMWPDTLVHW GRANGTAPSF FPQGRLLGGT SQIMGMFALR GRPEDYDDWA AMGAAGWGWD
     DVLPFFRKLE SDHDFSGTMH GCDGPVPIRR EPQAQWSPLS ARLHQVAAAR GFSHVADMNA
     DFGDGYGTLP ISRYPDKRGS AGICYLDAAV RARSNLTVQA GATVSRLLLE RRDGALHCTG
     VACTDAQGQV RQIRARRVLL AAGALRTPAL LLRAGIGPGD ELTRLGIAPQ LERKGVGRNL
     QNHAILYVVA FLARAGREPG GVRPACATTM RWSSAQHGGT PGDMGMYIRS WLSWHALGHR
     MASLAPVLNK PWSRGSVTLS AQAPQAQTVV EFDLLSDPRD LARITSAFRF TASLFGELGG
     ICGTPYVLSD ARNLSRLMRY NEPTSANAVR AAAAARLIDA MPGFGQRMVA KLSKMTPAAE
     VLRSDDALAD YARRSISGTG HVSGTCRMGA ASDPLAVVDP HGRVHGMQGL YVADASVMPT
     VPSANTHLPT IMVAEKIAQG LLV
//