ID F8S1G8_JAKLI Unreviewed; 1040 AA.
AC F8S1G8;
DT 21-SEP-2011, integrated into UniProtKB/TrEMBL.
DT 21-SEP-2011, sequence version 1.
DT 24-JAN-2024, entry version 36.
DE RecName: Full=DNA-directed RNA polymerase subunit {ECO:0000256|RuleBase:RU004279};
DE EC=2.7.7.6 {ECO:0000256|RuleBase:RU004279};
DE Flags: Fragment;
GN Name=RPB1 {ECO:0000313|EMBL:AEB78335.1};
OS Jakoba libera (Flagellate) (Cryptobia libera).
OC Eukaryota; Discoba; Jakobida; Histionina; Jakobidae; Jakoba.
OX NCBI_TaxID=143017 {ECO:0000313|EMBL:AEB78335.1};
RN [1] {ECO:0000313|EMBL:AEB78335.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC 50422 {ECO:0000313|EMBL:AEB78335.1};
RA Malik S.-B., Brochu C.D., Bilic I., Yuan J., Hess M., Logsdon J.M.Jr.,
RA Carlton J.M.;
RT "Phylogeny of Parasitic Parabasalia and Free-Living Relatives Inferred from
RT Conventional Markers vs. Rpb1, a Single-Copy Gene.";
RL PLoS ONE 6:E20774-E20774(2011).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000256|RuleBase:RU004279}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000256|ARBA:ARBA00024550,
CC ECO:0000256|RuleBase:RU004279};
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family.
CC {ECO:0000256|ARBA:ARBA00006460, ECO:0000256|RuleBase:RU004279}.
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DR EMBL; HQ436411; AEB78335.1; -; Genomic_DNA.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0043229; C:intracellular organelle; IEA:UniProt.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR GO; GO:0006351; P:DNA-templated transcription; IEA:InterPro.
DR CDD; cd02733; RNAP_II_RPB1_N; 1.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 2.40.40.20; -; 1.
DR Gene3D; 6.10.250.2940; -; 1.
DR Gene3D; 6.20.50.80; -; 1.
DR Gene3D; 3.30.1490.180; RNA polymerase ii; 1.
DR Gene3D; 4.10.860.120; RNA polymerase II, clamp domain; 1.
DR Gene3D; 1.10.274.100; RNA polymerase Rpb1, domain 3; 1.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR000722; RNA_pol_asu.
DR InterPro; IPR006592; RNA_pol_N.
DR InterPro; IPR007080; RNA_pol_Rpb1_1.
DR InterPro; IPR007066; RNA_pol_Rpb1_3.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR007075; RNA_pol_Rpb1_6.
DR InterPro; IPR044893; RNA_pol_Rpb1_clamp_domain.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; DNA-DIRECTED RNA POLYMERASE; 1.
DR PANTHER; PTHR19376:SF37; DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB1; 1.
DR Pfam; PF04997; RNA_pol_Rpb1_1; 1.
DR Pfam; PF00623; RNA_pol_Rpb1_2; 1.
DR Pfam; PF04983; RNA_pol_Rpb1_3; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 1.
DR Pfam; PF04992; RNA_pol_Rpb1_6; 1.
DR SMART; SM00663; RPOLA_N; 1.
DR SUPFAM; SSF64484; beta and beta-prime subunits of DNA dependent RNA-polymerase; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022478,
KW ECO:0000256|RuleBase:RU004279};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU004279};
KW Transcription {ECO:0000256|ARBA:ARBA00023163,
KW ECO:0000256|RuleBase:RU004279};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU004279}.
FT DOMAIN 157..452
FT /note="RNA polymerase N-terminal"
FT /evidence="ECO:0000259|SMART:SM00663"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:AEB78335.1"
FT NON_TER 1040
FT /evidence="ECO:0000313|EMBL:AEB78335.1"
SQ SEQUENCE 1040 AA; 116230 MW; 831102A92F39187E CRC64;
ECPGHFGHIE LAKPMYHVGY MNVVRKVLDC VCMKCSSPLF NPVSKRLGEL AKYCNSKVRM
CGDRSLAAAG AAGGAEGAEG QGQGAMDIDH GCGFTKPKIT RKEYFLMKEE KSPGQESGSQ
KVSQRILTAE EAHQVLKALT NEACEMLGFN PNFARPDWLL VTVLPVPPPA VRPSIDMGGS
GRAAQDDLTF KLGDILSTNQ LLRRQEEHGV NPQTLRNTEE MLQFHLFTFV DNELAGKSGK
VLKSISQRLK GKSGRIRNNL MGKRVDFSAR SVITADPILE IDQVGVPVDI AKIMTVPEVV
TPFNIKRLQE LVNHGPSVHP GARFVFMEDG TRQDLEFSPN FPLAYGYTVE RHLEDNDVVL
FNRQPTLHRM GMMGHRVKTM KFKTFRLNLS VTSPYNADFD GDEMNLHAPQ SLLTKAELLE
LMMIPKNMIS PKSNSPVMGL VQDSLLACRR ITLRDVFLER DHFMNCLMWV DDFDGQLPMP
AILKPKPLWT GKQLFSQILP KVNLRLRSKD HPRSENTLVS PGDTQVIIEQ GELLAGKCCK
NTMGTSQGSL VHVVYTEQGP EVCARMMGQV QRLVNYWLLT VGFSIGIGDC VPDEITMRTI
TEAITDDKKQ VTGVIKELHK RKLEKKPGLT LLESFEAVVN DKLNQARDKA GNAALESLSE
RNNFKNMVTA GSKGNELNIS QVIACVGQQN VEGRRIPFGF RNRTLPHFRQ ENYGPESKGF
VSNSYLSGLT PQEFFFHSMG GREGIIDTAV KTSETGYIQR RLVKAMEDIM VRYDGTVRDA
RGKVLQFVYG EDGLDSIRVE SQNFELVSMS QNAFRQLFEY PPHNHPLLSS SQSSFTVLLA
CIMVSRAQVI ESLRNDPTKV DELVDEVEQL REDRRALARI FTSGEGRSPM PVNVDRLIVN
AQKTFTIDMY SKSDLHPNDV PPVPSPWQVL RAVKKLCSDV MVTNIPDSEG SRNATYLFEI
YLRTKLASKR VIQKYHLDKQ SFEWLISEIR TRFFQALITP GECVGVIAAQ SVGEPATQMT
LNTFHFAGVS AKNVTXGVPM
//
