ID F8VQK3_MOUSE Unreviewed; 730 AA.
AC F8VQK3;
DT 21-SEP-2011, integrated into UniProtKB/TrEMBL.
DT 21-SEP-2011, sequence version 1.
DT 27-MAR-2024, entry version 88.
DE RecName: Full=guanylate cyclase {ECO:0000256|ARBA:ARBA00012202};
DE EC=4.6.1.2 {ECO:0000256|ARBA:ARBA00012202};
GN Name=Gucy1a2 {ECO:0000313|Ensembl:ENSMUSP00000111398.2,
GN ECO:0000313|MGI:MGI:2660877};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090 {ECO:0000313|Ensembl:ENSMUSP00000111398.2, ECO:0000313|Proteomes:UP000000589};
RN [1] {ECO:0000313|Ensembl:ENSMUSP00000111398.2, ECO:0000313|Proteomes:UP000000589}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J {ECO:0000313|Ensembl:ENSMUSP00000111398.2,
RC ECO:0000313|Proteomes:UP000000589};
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2] {ECO:0007829|PubMed:21183079}
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [3] {ECO:0000313|Ensembl:ENSMUSP00000111398.2}
RP IDENTIFICATION.
RC STRAIN=C57BL/6J {ECO:0000313|Ensembl:ENSMUSP00000111398.2};
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- SIMILARITY: Belongs to the adenylyl cyclase class-4/guanylyl cyclase
CC family. {ECO:0000256|RuleBase:RU000405}.
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DR RefSeq; NP_001028494.1; NM_001033322.2.
DR AlphaFoldDB; F8VQK3; -.
DR SMR; F8VQK3; -.
DR IntAct; F8VQK3; 1.
DR MINT; F8VQK3; -.
DR STRING; 10090.ENSMUSP00000111398; -.
DR GlyGen; F8VQK3; 4 sites, 1 O-linked glycan (4 sites).
DR iPTMnet; F8VQK3; -.
DR PhosphoSitePlus; F8VQK3; -.
DR SwissPalm; F8VQK3; -.
DR MaxQB; F8VQK3; -.
DR PaxDb; 10090-ENSMUSP00000111398; -.
DR PeptideAtlas; F8VQK3; -.
DR ProteomicsDB; 371483; -.
DR Antibodypedia; 4028; 141 antibodies from 18 providers.
DR DNASU; 234889; -.
DR Ensembl; ENSMUST00000115733.3; ENSMUSP00000111398.2; ENSMUSG00000041624.11.
DR GeneID; 234889; -.
DR KEGG; mmu:234889; -.
DR UCSC; uc009obc.2; mouse.
DR AGR; MGI:2660877; -.
DR CTD; 2977; -.
DR MGI; MGI:2660877; Gucy1a2.
DR VEuPathDB; HostDB:ENSMUSG00000041624; -.
DR eggNOG; KOG4171; Eukaryota.
DR GeneTree; ENSGT00940000157765; -.
DR HOGENOM; CLU_011614_5_0_1; -.
DR InParanoid; F8VQK3; -.
DR OMA; SLMLHYF; -.
DR OrthoDB; 2898719at2759; -.
DR PhylomeDB; F8VQK3; -.
DR TreeFam; TF351403; -.
DR Reactome; R-MMU-445355; Smooth Muscle Contraction.
DR BioGRID-ORCS; 234889; 2 hits in 77 CRISPR screens.
DR ChiTaRS; Gucy1a2; mouse.
DR Proteomes; UP000000589; Chromosome 9.
DR RNAct; F8VQK3; Protein.
DR Bgee; ENSMUSG00000041624; Expressed in embryonic post-anal tail and 46 other cell types or tissues.
DR Genevisible; F8VQK3; MM.
DR GO; GO:0008074; C:guanylate cyclase complex, soluble; IBA:GO_Central.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0004383; F:guanylate cyclase activity; ISO:MGI.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0044877; F:protein-containing complex binding; ISO:MGI.
DR GO; GO:0019934; P:cGMP-mediated signaling; IBA:GO_Central.
DR GO; GO:0007263; P:nitric oxide mediated signal transduction; IMP:MGI.
DR GO; GO:0010750; P:positive regulation of nitric oxide mediated signal transduction; IMP:MGI.
DR GO; GO:0070482; P:response to oxygen levels; IBA:GO_Central.
DR CDD; cd07302; CHD; 1.
DR Gene3D; 6.10.250.780; -; 1.
DR Gene3D; 3.90.1520.10; H-NOX domain; 1.
DR Gene3D; 3.30.450.260; Haem NO binding associated domain; 1.
DR Gene3D; 3.30.70.1230; Nucleotide cyclase; 1.
DR InterPro; IPR001054; A/G_cyclase.
DR InterPro; IPR018297; A/G_cyclase_CS.
DR InterPro; IPR038158; H-NOX_domain_sf.
DR InterPro; IPR011644; Heme_NO-bd.
DR InterPro; IPR011645; HNOB_dom_associated.
DR InterPro; IPR042463; HNOB_dom_associated_sf.
DR InterPro; IPR024096; NO_sig/Golgi_transp_ligand-bd.
DR InterPro; IPR029787; Nucleotide_cyclase.
DR PANTHER; PTHR45655:SF7; GUANYLATE CYCLASE SOLUBLE SUBUNIT ALPHA-2; 1.
DR PANTHER; PTHR45655; GUANYLATE CYCLASE SOLUBLE SUBUNIT BETA-2; 1.
DR Pfam; PF00211; Guanylate_cyc; 1.
DR Pfam; PF07700; HNOB; 1.
DR Pfam; PF07701; HNOBA; 1.
DR SMART; SM00044; CYCc; 1.
DR SUPFAM; SSF111126; Ligand-binding domain in the NO signalling and Golgi transport; 1.
DR SUPFAM; SSF55073; Nucleotide cyclase; 1.
DR PROSITE; PS00452; GUANYLATE_CYCLASE_1; 1.
DR PROSITE; PS50125; GUANYLATE_CYCLASE_2; 1.
PE 1: Evidence at protein level;
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW GTP-binding {ECO:0000256|ARBA:ARBA00023134};
KW Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000256|RuleBase:RU000405};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00023134};
KW Proteomics identification {ECO:0007829|MaxQB:F8VQK3,
KW ECO:0007829|PeptideAtlas:F8VQK3};
KW Reference proteome {ECO:0000313|Proteomes:UP000000589}.
FT DOMAIN 519..646
FT /note="Guanylate cyclase"
FT /evidence="ECO:0000259|PROSITE:PS50125"
FT REGION 1..56
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 460..491
FT /evidence="ECO:0000256|SAM:Coils"
FT COMPBIAS 1..20
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 730 AA; 81831 MW; C3907834F882505C CRC64;
MSRRKISSES FSSLSSDYLE TSPEEEGECP LSRLCWNGSR SPPGPPGSRE AAKAATSAPA
ASAAAASAAV AAEYKRAQRR GRVNLDSLGE SISLLTAPLP QTIHMTLKRT LQYYEHQVIG
YRDAEKNFHN ISKRCSYADH SNKEEIEDVS GILQCTANVL GLQFQEIQER FGEEFFKICF
DENERVLRAV GSTLQDFFNG FDALLEHIRT SFGKQATLES SSFLCKELPE GTLMLHYFHP
HHTVGFAMLG MIKAAGKRIY HLNVEVEQIE NEKLCFDGSN PSNCSCLTFL IKECETTQIT
KDNPQGTSQV PADLRISINT FCRAFPFHLM FDSNMVVLQL GEGLRKQLRY DTHRALKFED
CFEIVSPMIN ATFDRVLLRL STPFVIRTKP EASGTENEDK VMEIKGQMIH VPESSAILFL
GSPCVDKLDE LMGRGLHLSD IPIHDATRDV ILVGEQAKAQ DGLKKRMDKL KATLERTHQA
LEEEKKKTVD LLYSIFPGDV AQQLWQGQQV QARKFDDVTM LFSDIVGFTA ICAQCTPMQV
ISMLNELYTR FDHQCGLLDI YKVETIGDAY CVASGLHRKS LCHAKPIALM ALKMMELSEE
VLTPDGKAIQ MRIGIHSGSV LAGVVGVRMP RYCLFGNNVT LASKFESGSH PRRINVSPTT
YQLLKREDSF TFIPRSREEL PDNFPKEIPG VCYFLELRTD PKPPKPSLSS SRIKKVSYNI
GTMFLRETSL
//
