ID F9PN54_9ACTO Unreviewed; 1258 AA.
AC F9PN54;
DT 19-OCT-2011, integrated into UniProtKB/TrEMBL.
DT 19-OCT-2011, sequence version 1.
DT 24-JAN-2024, entry version 60.
DE RecName: Full=nitrate reductase (quinone) {ECO:0000256|ARBA:ARBA00012500};
DE EC=1.7.5.1 {ECO:0000256|ARBA:ARBA00012500};
GN ORFNames=HMPREF9058_0938 {ECO:0000313|EMBL:EGV12582.1};
OS Actinomyces sp. oral taxon 175 str. F0384.
OC Bacteria; Actinomycetota; Actinomycetes; Actinomycetales; Actinomycetaceae;
OC Actinomyces.
OX NCBI_TaxID=944560 {ECO:0000313|EMBL:EGV12582.1, ECO:0000313|Proteomes:UP000004281};
RN [1] {ECO:0000313|EMBL:EGV12582.1, ECO:0000313|Proteomes:UP000004281}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=F0384 {ECO:0000313|EMBL:EGV12582.1,
RC ECO:0000313|Proteomes:UP000004281};
RA Harkins D.M., Madupu R., Durkin A.S., Torralba M., Methe B., Sutton G.G.,
RA Nelson K.E.;
RL Submitted (JUL-2011) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a quinol + nitrate = a quinone + H2O + nitrite;
CC Xref=Rhea:RHEA:56144, ChEBI:CHEBI:15377, ChEBI:CHEBI:16301,
CC ChEBI:CHEBI:17632, ChEBI:CHEBI:24646, ChEBI:CHEBI:132124; EC=1.7.5.1;
CC Evidence={ECO:0000256|ARBA:ARBA00001854};
CC -!- COFACTOR:
CC Name=Mo-bis(molybdopterin guanine dinucleotide);
CC Xref=ChEBI:CHEBI:60539; Evidence={ECO:0000256|ARBA:ARBA00001942};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004202};
CC Peripheral membrane protein {ECO:0000256|ARBA:ARBA00004202}. Membrane
CC {ECO:0000256|ARBA:ARBA00004170}; Peripheral membrane protein
CC {ECO:0000256|ARBA:ARBA00004170}.
CC -!- SIMILARITY: Belongs to the prokaryotic molybdopterin-containing
CC oxidoreductase family. {ECO:0000256|ARBA:ARBA00010312}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EGV12582.1}.
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DR EMBL; AFUR01000006; EGV12582.1; -; Genomic_DNA.
DR RefSeq; WP_009747853.1; NZ_AFUR01000006.1.
DR AlphaFoldDB; F9PN54; -.
DR eggNOG; COG5013; Bacteria.
DR Proteomes; UP000004281; Unassembled WGS sequence.
DR GO; GO:0009325; C:nitrate reductase complex; IEA:InterPro.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0043546; F:molybdopterin cofactor binding; IEA:InterPro.
DR GO; GO:0008940; F:nitrate reductase activity; IEA:InterPro.
DR GO; GO:0042126; P:nitrate metabolic process; IEA:InterPro.
DR CDD; cd02776; MopB_CT_Nitrate-R-NarG-like; 1.
DR Gene3D; 3.40.50.12440; -; 1.
DR InterPro; IPR009010; Asp_de-COase-like_dom_sf.
DR InterPro; IPR037943; MopB_CT_Nitrate-R-NarG-like.
DR InterPro; IPR006657; MoPterin_dinucl-bd_dom.
DR InterPro; IPR006656; Mopterin_OxRdtase.
DR InterPro; IPR006963; Mopterin_OxRdtase_4Fe-4S_dom.
DR InterPro; IPR006655; Mopterin_OxRdtase_prok_CS.
DR InterPro; IPR027467; MopterinOxRdtase_cofactor_BS.
DR InterPro; IPR006468; NarG.
DR NCBIfam; TIGR01580; narG; 1.
DR PANTHER; PTHR43105; RESPIRATORY NITRATE REDUCTASE; 1.
DR PANTHER; PTHR43105:SF2; RESPIRATORY NITRATE REDUCTASE 2 ALPHA CHAIN; 1.
DR Pfam; PF00384; Molybdopterin; 1.
DR Pfam; PF01568; Molydop_binding; 1.
DR SMART; SM00926; Molybdop_Fe4S4; 1.
DR SUPFAM; SSF50692; ADC-like; 1.
DR SUPFAM; SSF53706; Formate dehydrogenase/DMSO reductase, domains 1-3; 1.
DR PROSITE; PS51669; 4FE4S_MOW_BIS_MGD; 1.
DR PROSITE; PS00551; MOLYBDOPTERIN_PROK_1; 1.
DR PROSITE; PS00490; MOLYBDOPTERIN_PROK_2; 1.
PE 3: Inferred from homology;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Membrane {ECO:0000256|ARBA:ARBA00022475};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Oxidoreductase {ECO:0000313|EMBL:EGV12582.1}.
FT DOMAIN 65..129
FT /note="4Fe-4S Mo/W bis-MGD-type"
FT /evidence="ECO:0000259|PROSITE:PS51669"
FT REGION 1..24
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1258 AA; 138186 MW; B9B2AA4DE14D3C3A CRC64;
MSTTGSQTTG PGIVPGPDQQ VENAPGLLTL GSYLRRGQAS ADARRLFLTG GREADTFYRH
RWSHDKMVHS THGVNCTGSC AWEVYVSDGI ITWEKQITDY PTTGPDMPEY EPRGCPRGAA
FSWYTYSPTR IRYPYVRSVL LDAFRAAKAD NDGDPVAAWA QVTGDPATAK AYKSARGKGG
MVRVGWDEAM EIVAAAYVHT IRAWGPDRIA GFSVIPAMSQ VSYGAGGRLH ELIGGTMLSF
YDWYADLPPA SPQVFGDQTD VPEAGDWYNS QYLMMWGSNL PLTRTPDAHF MTEARYHGQK
VVAVSPDYAD NTKFADQWLR VAPGTDGALA QAMGHVILSE FHVKREESFF LDYMRRHTDS
PFLISLDPSP DGTGYVPGRF VTASDVNGVA TGAPKNEFRP LVWDRERGPA DPGGTLADRF
TPEGEGKWNL LMEGVDPVMS ILDLHGQGPQ VQAAEVLLPR FDLPGSSTPE GSVGGGVVRR
GVPVTRIGER MVTTVYDLLL AQYAVERPSM PGEWPADYQD ATVPGTPAWA SEITGVPGPA
IIQVARDFAL NAVESGGRSQ IVMGAGINHY YHADQIYRTI LALTSMCATQ GVNGGGWAHY
VGQEKVRPLS GFQQYAFALD WHRPARQMIS TGFWYLTTDQ WRYDNTSAER LASPLGPGTL
AGKTTTDAMV EAMKRGWTPS YPTFNRSPLL LGQQAAEAGM DPKDYIVEQL RSGELRFACE
DPDAPENFPR ILCSWRTNLL GSSAKGTEFF LRHMVGADND VNAVETPAEQ RPASVTWRDE
APVGKLDLMW TADFRNTSTT LHSDVVLPAA TWYEKHDISS TDMHPFVHSF NAAIDPPWEA
RTDFQVFQTL AGLISAWAPR YLGTQTDVVA APLTHDTPDA LTMAHGDVSS LPQEWVPGVT
MPKLVPTERD YTQIRSKFDA LGPLAEKLGI PCKGIMLKPG PEVERLARNH GVSSDGVAAG
RPLLDTDIRA ADAIFTLSGT TNGRIATEGW DTLSKRTGTP LVELSEEEAG KHISFADTQV
KPQGVITSPE WSGSEHGGRR YSAFVVNVEH AKPWHTLTGR MHYYLDHDWM RDMGESLPIF
RPPLDFHALY GEAAPGAVST SQAGTAEVAV RYITAHNKWA IHSQYFDNLH MLTLGRGGQT
IWMSPQDADK IGVKDNEWVE AYNRNGIVAA RAIVSHRIPE GMVFMHHAQE RTMNTPLTET
SGRRGGTHNS LTRIVLKPSH FAGGYGQLSY AFNYIGPTGN NRDEVTLIRR RSNQEVTF
//
