ID F9USV7_LACPL Unreviewed; 282 AA.
AC F9USV7;
DT 19-OCT-2011, integrated into UniProtKB/TrEMBL.
DT 19-OCT-2011, sequence version 1.
DT 27-MAR-2024, entry version 63.
DE SubName: Full=2,5 diketo-D-gluconic acid-like reductase,NADP dependent, promiscuous {ECO:0000313|EMBL:CCC77688.1};
DE EC=1.1.1.- {ECO:0000313|EMBL:CCC77688.1};
GN OrderedLocusNames=lp_0136 {ECO:0000313|EMBL:CCC77688.1};
OS Lactiplantibacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1)
OS (Lactobacillus plantarum).
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae;
OC Lactiplantibacillus.
OX NCBI_TaxID=220668 {ECO:0000313|EMBL:CCC77688.1, ECO:0000313|Proteomes:UP000000432};
RN [1] {ECO:0000313|EMBL:CCC77688.1, ECO:0000313|Proteomes:UP000000432}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-793 / NCIMB 8826 / WCFS1
RC {ECO:0000313|Proteomes:UP000000432};
RX PubMed=12566566; DOI=10.1073/pnas.0337704100;
RA Kleerebezem M., Boekhorst J., van Kranenburg R., Molenaar D., Kuipers O.P.,
RA Leer R., Tarchini R., Peters S.A., Sandbrink H.M., Fiers M.W., Stiekema W.,
RA Lankhorst R.M., Bron P.A., Hoffer S.M., Groot M.N., Kerkhoven R.,
RA de Vries M., Ursing B., de Vos W.M., Siezen R.J.;
RT "Complete genome sequence of Lactobacillus plantarum WCFS1.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:1990-1995(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=WCFS1;
RA Siezen R.J., Francke C., Renckens B., Boekhorst J., Wels M.,
RA Kleerebezem M., van Hijum S.A.F.T.;
RT "Complete resequencing and reannotation of the Lactobacillus plantarum
RT WCFS1 genome.";
RL Submitted (JUN-2011) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:CCC77688.1, ECO:0000313|Proteomes:UP000000432}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-793 / NCIMB 8826 / WCFS1
RC {ECO:0000313|Proteomes:UP000000432};
RX PubMed=22156394; DOI=10.1128/JB.06275-11;
RA Siezen R.J., Francke C., Renckens B., Boekhorst J., Wels M.,
RA Kleerebezem M., van Hijum S.A.F.T.;
RT "Complete resequencing and reannotation of the Lactobacillus plantarum
RT WCFS1 genome.";
RL J. Bacteriol. 194:195-196(2012).
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AL935263; CCC77688.1; -; Genomic_DNA.
DR RefSeq; WP_003646328.1; NC_004567.2.
DR RefSeq; YP_004888202.1; NC_004567.2.
DR AlphaFoldDB; F9USV7; -.
DR SMR; F9USV7; -.
DR STRING; 220668.lp_0136; -.
DR EnsemblBacteria; CCC77688; CCC77688; lp_0136.
DR GeneID; 79831143; -.
DR KEGG; lpl:lp_0136; -.
DR PATRIC; fig|220668.9.peg.112; -.
DR eggNOG; COG0656; Bacteria.
DR HOGENOM; CLU_023205_0_1_9; -.
DR OrthoDB; 9804790at2; -.
DR PhylomeDB; F9USV7; -.
DR Proteomes; UP000000432; Chromosome.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR GO; GO:0071704; P:organic substance metabolic process; IEA:UniProt.
DR GO; GO:0044281; P:small molecule metabolic process; IEA:UniProt.
DR CDD; cd19071; AKR_AKR1-5-like; 1.
DR Gene3D; 3.20.20.100; NADP-dependent oxidoreductase domain; 1.
DR InterPro; IPR020471; AKR.
DR InterPro; IPR018170; Aldo/ket_reductase_CS.
DR InterPro; IPR023210; NADP_OxRdtase_dom.
DR InterPro; IPR036812; NADP_OxRdtase_dom_sf.
DR PANTHER; PTHR43827; 2,5-DIKETO-D-GLUCONIC ACID REDUCTASE; 1.
DR PANTHER; PTHR43827:SF3; ALDO-KETO REDUCTASE; 1.
DR Pfam; PF00248; Aldo_ket_red; 1.
DR PIRSF; PIRSF000097; AKR; 1.
DR PRINTS; PR00069; ALDKETRDTASE.
DR SUPFAM; SSF51430; NAD(P)-linked oxidoreductase; 1.
DR PROSITE; PS00798; ALDOKETO_REDUCTASE_1; 1.
DR PROSITE; PS00062; ALDOKETO_REDUCTASE_2; 1.
PE 4: Predicted;
KW Oxidoreductase {ECO:0000313|EMBL:CCC77688.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000000432}.
FT DOMAIN 18..267
FT /note="NADP-dependent oxidoreductase"
FT /evidence="ECO:0000259|Pfam:PF00248"
FT ACT_SITE 53
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR000097-1"
FT BINDING 111
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR000097-2"
FT SITE 78
FT /note="Lowers pKa of active site Tyr"
FT /evidence="ECO:0000256|PIRSR:PIRSR000097-3"
SQ SEQUENCE 282 AA; 30820 MW; 06889AF05A74357C CRC64;
MTVLTTMYQL ANGVNIPKVA FGTWQIPGGE ATYQAVRAAL AAGYRHIDTA LAYQNEASVG
QAIRDSGIPR EQIFVTTKLP AETKSYQGAL NDFDRSLKNL GLDYVDLYLV HAPWPWGQVG
RVYDEANLDV WQAMEAIYQS GRAKAIGVSN FAVRDLKNIL NQATIPPMVN QIQYYLGFTE
PKITKFSEEH AMLVEAYSPL ATGGVLDNPQ VQAYADKYGV SVAQLALRFV LQNGVLPLPK
AVNPAHIEAN TQLDFAISVA DMQALNDLPD AAGSYMHNPT QG
//
