ID FIXB_SINFN Reviewed; 369 AA.
AC Q53209;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 27-MAR-2024, entry version 127.
DE RecName: Full=Protein FixB;
GN Name=fixB; OrderedLocusNames=NGR_a01230; ORFNames=y4vA;
OS Sinorhizobium fredii (strain NBRC 101917 / NGR234).
OG Plasmid sym pNGR234a.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX NCBI_TaxID=394;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8796346; DOI=10.1101/gr.6.7.590;
RA Freiberg C., Perret X., Broughton W.J., Rosenthal A.;
RT "Sequencing the 500-kb GC-rich symbiotic replicon of Rhizobium sp. NGR234
RT using dye terminators and a thermostable 'sequenase': a beginning.";
RL Genome Res. 6:590-600(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NBRC 101917 / NGR234;
RX PubMed=9163424; DOI=10.1038/387394a0;
RA Freiberg C.A., Fellay R., Bairoch A., Broughton W.J., Rosenthal A.,
RA Perret X.;
RT "Molecular basis of symbiosis between Rhizobium and legumes.";
RL Nature 387:394-401(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NBRC 101917 / NGR234;
RX PubMed=19376903; DOI=10.1128/aem.00515-09;
RA Schmeisser C., Liesegang H., Krysciak D., Bakkou N., Le Quere A.,
RA Wollherr A., Heinemeyer I., Morgenstern B., Pommerening-Roeser A.,
RA Flores M., Palacios R., Brenner S., Gottschalk G., Schmitz R.A.,
RA Broughton W.J., Perret X., Strittmatter A.W., Streit W.R.;
RT "Rhizobium sp. strain NGR234 possesses a remarkable number of secretion
RT systems.";
RL Appl. Environ. Microbiol. 75:4035-4045(2009).
CC -!- FUNCTION: May play a role in a redox process involved in nitrogen
CC fixation.
CC -!- SUBUNIT: FixA and FixB form a heterodimer. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the ETF alpha-subunit/FixB family.
CC {ECO:0000305}.
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DR EMBL; Z68203; CAA92416.1; -; Genomic_DNA.
DR EMBL; U00090; AAB91889.1; -; Genomic_DNA.
DR RefSeq; NP_444102.1; NC_000914.2.
DR RefSeq; WP_010875161.1; NC_000914.2.
DR AlphaFoldDB; Q53209; -.
DR SMR; Q53209; -.
DR KEGG; rhi:NGR_a01230; -.
DR PATRIC; fig|394.7.peg.107; -.
DR eggNOG; COG2025; Bacteria.
DR HOGENOM; CLU_034178_1_1_5; -.
DR OrthoDB; 9770286at2; -.
DR Proteomes; UP000001054; Plasmid sym pNGR234a.
DR GO; GO:0009055; F:electron transfer activity; IEA:InterPro.
DR GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
DR GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR CDD; cd01715; ETF_alpha; 1.
DR Gene3D; 3.40.50.620; HUPs; 1.
DR Gene3D; 3.40.50.1220; TPP-binding domain; 1.
DR InterPro; IPR029035; DHS-like_NAD/FAD-binding_dom.
DR InterPro; IPR014730; ETF_a/b_N.
DR InterPro; IPR001308; ETF_a/FixB.
DR InterPro; IPR033947; ETF_alpha_N.
DR InterPro; IPR014731; ETF_asu_C.
DR InterPro; IPR018206; ETF_asu_C_CS.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR PANTHER; PTHR43153; ELECTRON TRANSFER FLAVOPROTEIN ALPHA; 1.
DR PANTHER; PTHR43153:SF1; ELECTRON TRANSFER FLAVOPROTEIN SUBUNIT ALPHA, MITOCHONDRIAL; 1.
DR Pfam; PF01012; ETF; 1.
DR Pfam; PF00766; ETF_alpha; 1.
DR PIRSF; PIRSF000089; Electra_flavoP_a; 1.
DR SMART; SM00893; ETF; 1.
DR SUPFAM; SSF52402; Adenine nucleotide alpha hydrolases-like; 1.
DR SUPFAM; SSF52467; DHS-like NAD/FAD-binding domain; 1.
DR PROSITE; PS00696; ETF_ALPHA; 1.
PE 3: Inferred from homology;
KW Electron transport; FAD; Flavoprotein; Nitrogen fixation; Plasmid;
KW Reference proteome; Transport.
FT CHAIN 1..369
FT /note="Protein FixB"
FT /id="PRO_0000167859"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 299..327
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000255"
SQ SEQUENCE 369 AA; 39851 MW; C4B55DB56FA1C08A CRC64;
MGTRKSKASP PAQARASMKK DLPEQFRDYR HVWVFMELER GHVHPVSFEL LGEGRRLADQ
LGVQLAGIIL GPPGDSTLHA AAEAFAYGAD LVYLVEEPLL TDYRNEPFTK ALTDLVITHK
PEILLLGATT LGRDLAGSVA TTLLTGLTAD CTGLDVDEDG SLAATRPTFG GSLLCTIYTL
NCRPQMATVR PRVMRMPQRS NKPIGRVIRH DWRMLEEDIV TKVVGFLSDD RSGNANLAYA
DVVVAGGLGL SNAENLLLIK DLALTIGGDY GCSRPLVQKG WMPADRQIGQ TGKTIRPKLY
IAAGISGAVQ HRVGVEGADL IVAINTDPHA PIFDFAHVGV VTDAVSFLPA LTEAFTKRLA
PANRLRVVN
//
