ID G0UT19_TRYCI Unreviewed; 1754 AA.
AC G0UT19;
DT 19-OCT-2011, integrated into UniProtKB/TrEMBL.
DT 19-OCT-2011, sequence version 1.
DT 24-JAN-2024, entry version 36.
DE RecName: Full=DNA-directed RNA polymerase subunit {ECO:0000256|RuleBase:RU004279};
DE EC=2.7.7.6 {ECO:0000256|RuleBase:RU004279};
GN ORFNames=TCIL3000_8_7610 {ECO:0000313|EMBL:CCC92532.1};
OS Trypanosoma congolense (strain IL3000).
OC Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina;
OC Trypanosomatida; Trypanosomatidae; Trypanosoma; Nannomonas.
OX NCBI_TaxID=1068625 {ECO:0000313|EMBL:CCC92532.1};
RN [1] {ECO:0000313|EMBL:CCC92532.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=IL3000 {ECO:0000313|EMBL:CCC92532.1};
RX PubMed=22331916; DOI=10.1073/pnas.1117313109;
RA Jackson A.P., Berry A., Aslett M., Allison H.C., Burton P.,
RA Vavrova-Anderson J., Brown R., Browne H., Corton N., Hauser H., Gamble J.,
RA Gilderthorp R., Marcello L., McQuillan J., Otto T.D., Quail M.A.,
RA Sanders M.J., van Tonder A., Ginger M.L., Field M.C., Barry J.D.,
RA Hertz-Fowler C., Berriman M.;
RT "Antigenic diversity is generated by distinct evolutionary mechanisms in
RT African trypanosome species.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:3416-3421(2012).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000256|RuleBase:RU004279}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000256|ARBA:ARBA00024550,
CC ECO:0000256|RuleBase:RU004279};
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family.
CC {ECO:0000256|ARBA:ARBA00006460, ECO:0000256|RuleBase:RU004279}.
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DR EMBL; HE575321; CCC92532.1; -; Genomic_DNA.
DR OrthoDB; 169836at2759; -.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0043229; C:intracellular organelle; IEA:UniProt.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR GO; GO:0006351; P:DNA-templated transcription; IEA:InterPro.
DR CDD; cd02733; RNAP_II_RPB1_N; 1.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.150.390; -; 1.
DR Gene3D; 2.40.40.20; -; 1.
DR Gene3D; 3.30.1360.140; -; 1.
DR Gene3D; 6.10.250.2940; -; 1.
DR Gene3D; 6.20.50.80; -; 1.
DR Gene3D; 3.30.1490.180; RNA polymerase ii; 1.
DR Gene3D; 4.10.860.120; RNA polymerase II, clamp domain; 1.
DR Gene3D; 1.10.274.100; RNA polymerase Rpb1, domain 3; 1.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR000722; RNA_pol_asu.
DR InterPro; IPR006592; RNA_pol_N.
DR InterPro; IPR007080; RNA_pol_Rpb1_1.
DR InterPro; IPR007066; RNA_pol_Rpb1_3.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR007075; RNA_pol_Rpb1_6.
DR InterPro; IPR007073; RNA_pol_Rpb1_7.
DR InterPro; IPR038593; RNA_pol_Rpb1_7_sf.
DR InterPro; IPR044893; RNA_pol_Rpb1_clamp_domain.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; DNA-DIRECTED RNA POLYMERASE; 1.
DR PANTHER; PTHR19376:SF37; DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB1; 1.
DR Pfam; PF04997; RNA_pol_Rpb1_1; 1.
DR Pfam; PF00623; RNA_pol_Rpb1_2; 1.
DR Pfam; PF04983; RNA_pol_Rpb1_3; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 1.
DR Pfam; PF04992; RNA_pol_Rpb1_6; 1.
DR Pfam; PF04990; RNA_pol_Rpb1_7; 1.
DR SMART; SM00663; RPOLA_N; 1.
DR SUPFAM; SSF64484; beta and beta-prime subunits of DNA dependent RNA-polymerase; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022478,
KW ECO:0000256|RuleBase:RU004279};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU004279};
KW Transcription {ECO:0000256|ARBA:ARBA00023163,
KW ECO:0000256|RuleBase:RU004279};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU004279}.
FT DOMAIN 242..534
FT /note="RNA polymerase N-terminal"
FT /evidence="ECO:0000259|SMART:SM00663"
FT REGION 1633..1662
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1697..1754
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1754 AA; 195453 MW; C7BE1A55CE405B14 CRC64;
MSGGAPLPPS HMELQKVNEV QFEIFKERQI KSYAVCLVEH AKSYERGKPV RGGINDLRMG
TTDFEFACET CHRKHPECPG HFGYIELAEP VFNIGVFDLV LQVLKCVCKS CGALLLNTRA
DDVHKKLHNL TGLSRLRQAA KMAETKCRMS TDLEDEVGDD GLNGGSFGGG GAVAGARAAR
GCGASQPRVS RFYGIYPTLI VKAVHGDQDA EWHADKVRQV LDRVSDEDAR LMGFDPLRCH
PRDLVLTVLP VPPPQVRPAI SFGGLKSDDE LTHQIMAIVK RNNQLRKDKE SDVQAAIDRS
RALLQEHVAT YFNNSSTYYK PTKVNDSKKL KSLTERLKGK YGRLRGNLMG KRVDFSARTV
ITGDPNIDVD EVGVPFSVAM TLTFPERVNT VNKKRLTEFV RREVYPSANY IHHPNGTITK
LALLRDRSKV TLNIGDIVER HVINGDVVLF NRQPTLHRMS MMGHRVRVLN YNTFRLNLSC
TTPYNADFDG DEMNLHVPQS LLTKAELIEM MMVPKNFVSP TSQRRAWELC RTVCSEATGD
KDTFLDKYFV QSVALWLDLW QLPIPAVLKP RPLWTGKQVF SLILPEVNHP TTPHDKPPFP
HNDSSVMIRR GELLCGPITK GIVGAAPGSL IHVIFNEQGS DEVARFINGV QRVTTFFLLN
FGFSVGVQDT VADTDTLRQM NEVLVKTREN VEKIGAAANN RTLNRKAGMT LLQSFEADVN
SALNKCREEA AKKALSNVRR TNSFKVMIEA GSKGTDLNIC QIAVFVGQQN VAGSRIPFGF
RRRTLPHFML DDYGETSRGM ANRGYVEGLK PHEFFFHTMA GREGLIDTAV KTSDTGYLQR
KLIKALEDVH AAYDGTVRNA NEELIQFMYG EDGLDGARIE GGQLFPLPFR DNKEMEQMYK
YEYEADGTFT GKVGGNYMDP HVKKMLRADP ENVRKLHGEY EQLMADREWS RKMLDLEDRE
KLKLNLPVNP GRLIQNARST MGKRSQVSNL SPITIIDHVR KLQEDLMKLF PSYHRDADRR
ITNVLSRQRI ESALTLFNVH LRQLLASKRV LKEYKLNDKA FEYLLKEIRT KYHQSLATPG
ENIGAIAAQS CGEPATQMTL NTFHNAGISS KNVTLGVPRL LELLNVSRHQ KHASMTVTLF
PPWDKKRVAQ KAQHLIEYCT LESITQRIQF IYDPDPRHTV VEVDRDILEL EWDVMDESDA
EQRVREVEEG SPWVVRLELD VDMVTDKALD MKDVKQAIQR VDTNYIIETG MANNVRRRTI
RLRSRTNDGA DSIPKLKRDI PALLARVHLR GIPGVRRALL KDTTKFTVDE STGKLTGEKT
WVIDTDGTAL RRAFIGVVGE DGKNIINATK TSSNKVPEVC SLLGIEAARY RMLTELREAY
LAYGLNINYR HYTILVDTIC QRGYLMAVSR TGINRSDTSG PLMRCSFEET VKVLMAAASF
GEHDPVRGVS ANLVLGNQAR VGTGLFDLII NMPALQQAVT QEDAVAPGKN VNVYHGLGST
LQQNLQSSMV YRPRENDATP FVNDASIFFH GGPAGGSSSA PATASAPLNP SLPYGGRFEA
STVHHSQIYP SASVTEYSAR EVSYSQQYSV ASASAFNPIS THLPSTAQVS VDYSAMSSYH
LQYSVAAPSM HTSFPRSETS MATPSANREQ STSAPYMPSA MSSTALPSHA YASTEVDLLP
SDNSRSQSAL YVPTFSPAHA GASTHHNEPG DRQRAGGTAA AEDEDLSVNY LPTTQVQSSA
VPPAEYVDEE EEEQ
//
