ID G0W8A2_NAUDC Unreviewed; 1263 AA.
AC G0W8A2;
DT 19-OCT-2011, integrated into UniProtKB/TrEMBL.
DT 19-OCT-2011, sequence version 1.
DT 27-MAR-2024, entry version 50.
DE RecName: Full=RNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00012493};
DE EC=2.7.7.49 {ECO:0000256|ARBA:ARBA00012493};
GN Name=NDAI0C03530 {ECO:0000313|EMBL:CCD24013.1};
GN OrderedLocusNames=NDAI_0C03530 {ECO:0000313|EMBL:CCD24013.1};
OS Naumovozyma dairenensis (strain ATCC 10597 / BCRC 20456 / CBS 421 / NBRC
OS 0211 / NRRL Y-12639) (Saccharomyces dairenensis).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Naumovozyma.
OX NCBI_TaxID=1071378 {ECO:0000313|EMBL:CCD24013.1, ECO:0000313|Proteomes:UP000000689};
RN [1] {ECO:0000313|EMBL:CCD24013.1, ECO:0000313|Proteomes:UP000000689}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 10597 / BCRC 20456 / CBS 421 / NBRC 0211 / NRRL Y-12639
RC {ECO:0000313|Proteomes:UP000000689};
RX PubMed=22123960; DOI=10.1073/pnas.1112808108;
RA Gordon J.L., Armisen D., Proux-Wera E., OhEigeartaigh S.S., Byrne K.P.,
RA Wolfe K.H.;
RT "Evolutionary erosion of yeast sex chromosomes by mating-type switching
RT accidents.";
RL Proc. Natl. Acad. Sci. U.S.A. 108:20024-20029(2011).
CC -!- FUNCTION: Integrase (IN) targets the VLP to the nucleus, where a
CC subparticle preintegration complex (PIC) containing at least integrase
CC and the newly synthesized dsDNA copy of the retrotransposon must
CC transit the nuclear membrane. Once in the nucleus, integrase performs
CC the integration of the dsDNA into the host genome.
CC {ECO:0000256|ARBA:ARBA00025615}.
CC -!- FUNCTION: Reverse transcriptase/ribonuclease H (RT) is a
CC multifunctional enzyme that catalyzes the conversion of the retro-
CC elements RNA genome into dsDNA within the VLP. The enzyme displays a
CC DNA polymerase activity that can copy either DNA or RNA templates, and
CC a ribonuclease H (RNase H) activity that cleaves the RNA strand of RNA-
CC DNA heteroduplexes during plus-strand synthesis and hydrolyzes RNA
CC primers. The conversion leads to a linear dsDNA copy of the
CC retrotransposon that includes long terminal repeats (LTRs) at both
CC ends. {ECO:0000256|ARBA:ARBA00025590}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endonucleolytic cleavage to 5'-phosphomonoester.; EC=3.1.26.4;
CC Evidence={ECO:0000256|ARBA:ARBA00000077};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC Nucleus {ECO:0000256|ARBA:ARBA00004123}.
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DR EMBL; HE580269; CCD24013.1; -; Genomic_DNA.
DR RefSeq; XP_003669256.1; XM_003669208.1.
DR AlphaFoldDB; G0W8A2; -.
DR STRING; 1071378.G0W8A2; -.
DR GeneID; 11494758; -.
DR KEGG; ndi:NDAI_0C03530; -.
DR eggNOG; KOG0017; Eukaryota.
DR HOGENOM; CLU_000384_38_1_1; -.
DR OMA; GHNTIWA; -.
DR OrthoDB; 2038104at2759; -.
DR Proteomes; UP000000689; Chromosome 3.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0004523; F:RNA-DNA hybrid ribonuclease activity; IEA:UniProtKB-EC.
DR GO; GO:0015074; P:DNA integration; IEA:InterPro.
DR CDD; cd00303; retropepsin_like; 1.
DR CDD; cd09274; RNase_HI_RT_Ty3; 1.
DR CDD; cd01647; RT_LTR; 1.
DR Gene3D; 1.10.340.70; -; 1.
DR Gene3D; 3.30.70.270; -; 2.
DR Gene3D; 2.40.70.10; Acid Proteases; 1.
DR Gene3D; 3.10.10.10; HIV Type 1 Reverse Transcriptase, subunit A, domain 1; 1.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR001584; Integrase_cat-core.
DR InterPro; IPR041588; Integrase_H2C2.
DR InterPro; IPR024650; Peptidase_A2B.
DR InterPro; IPR021109; Peptidase_aspartic_dom_sf.
DR InterPro; IPR043128; Rev_trsase/Diguanyl_cyclase.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR InterPro; IPR000477; RT_dom.
DR InterPro; IPR041373; RT_RNaseH.
DR PANTHER; PTHR37984:SF7; INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN; 1.
DR PANTHER; PTHR37984; PROTEIN CBG26694; 1.
DR Pfam; PF17921; Integrase_H2C2; 1.
DR Pfam; PF12384; Peptidase_A2B; 1.
DR Pfam; PF17917; RT_RNaseH; 1.
DR Pfam; PF00665; rve; 1.
DR Pfam; PF00078; RVT_1; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
DR PROSITE; PS50994; INTEGRASE; 1.
DR PROSITE; PS50878; RT_POL; 1.
PE 4: Predicted;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Reference proteome {ECO:0000313|Proteomes:UP000000689};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884};
KW Transposable element {ECO:0000256|ARBA:ARBA00022464}.
FT DOMAIN 324..501
FT /note="Reverse transcriptase"
FT /evidence="ECO:0000259|PROSITE:PS50878"
FT DOMAIN 882..1054
FT /note="Integrase catalytic"
FT /evidence="ECO:0000259|PROSITE:PS50994"
SQ SEQUENCE 1263 AA; 146773 MW; 236AB971706D8F47 CRC64;
MTFVETVMNM VIIRNNARNP MRGLCKANEE LEATVAIPEC SSNDDLLVCN LESHTREDPL
LTCNLESHEK EDPRVTTTKI NPPVEDVCIV NIGTKSRTLE ALIDTGSPTH FIRSDVVTKL
DLKTSQVPFR RVKGLVSDAI TTCNTACRLD FRLENREFDI CAYVTDIIKN DVLIGYPFVK
RHPSLMESIH KNIDNVKFNN RPVSDPGRMN YEDVTYGKDP IDDICNIETD ENWIDIQRDA
SDVIIVEVTE VTNKDESKFD TIPEELQVKY RGIVRNDLPP RQRDHKHVSH SIELKEGSRL
PRRSPYRLTP KKQKEVDEII KDLLDKGFIV PSKSSYSSPI VLVTKHDGSY RLCVDYRELN
KVTVKDPFPL PHVDELLGKV GSASVFTTLD LHSGYHQIPM NPTDMDKTAF VTPTGKYEYT
VMPFGLVNAP STFARYMADL FRDLEFVNVY LDDILIFSND LESHWKHIDV VLSRLDQEKL
IAKKKKCHFA QSEVQFLGYI IGRNKIKPVQ EKCEAINRFP VPKTIKEAQR FVGMINYYRK
FIKDCSRKVR PLVDFISRNV PWGDLQDDAF ATLKRDLMSE PLLVPFKRDA EYRLTTDASM
DGLGAVLEEV ADNKVLGVVS YYSKSLNETQ RRYPPGELEL MAIIEGLEHF KYMLHGKHFV
LRTDHISLLS IQNQKEPARR VQRWLDTLSE FDFSLAYLPG PKNVVADAIS RAKLENKEVS
NEPVNDYKEI LTVATADTLH TLDPESWTTD WRTDPWGAAV LKSLDDKFDH EIPTEQVNEF
TRYLKKFERT PEYLKHFKWT NDVLYYEDRI CVPHIRRPLV METYHDHKWF GGHFGEHDTF
KKISEIYFWP NCYKTVQDYV KSCIQCQVMK AHRPRSQGLH KPLSVPSGRW LDISMDFLTG
IPTTLAGWDM IMVVIDRFTK RAHFVACKKV NGSTGVFDAL FRFVFSLHGF PRTIVSDRDI
RFTSNAYREL TDRLGIKLLM STSNHPQTDG QTERVNRTLN QLLRMYCSND QSCWDKLLPH
VEYVYNSTYQ RVLCMSPFEA DLGYKPNEPR MNRDYIINAR HLTSAEYARD IDALTLRIKD
QLEENQLRQE YDANKNRTPV NYKIGDYVLL HRDAYFTGGQ YRKIQAIYLG PFQVVGVGTN
CCELDLPSMR KLHRMINVTW LKPYVERTNM YPKWKPRAKI ERLQRLEEIT SIIGYSLNDK
IYYCKMKDVD PRLTVEYPEE ELRNLSKPRL DSLLRNFNQL ETEEEKPVEW ARVGCLKVWG
RRM
//
