ID G1KHK6_ANOCA Unreviewed; 1530 AA.
AC G1KHK6;
DT 19-OCT-2011, integrated into UniProtKB/TrEMBL.
DT 29-SEP-2021, sequence version 3.
DT 27-MAR-2024, entry version 71.
DE RecName: Full=RNA uridylyltransferase {ECO:0000256|ARBA:ARBA00012472};
DE EC=2.7.7.52 {ECO:0000256|ARBA:ARBA00012472};
GN Name=TUT4 {ECO:0000313|Ensembl:ENSACAP00000008110.4};
OS Anolis carolinensis (Green anole) (American chameleon).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC Iguania; Dactyloidae; Anolis.
OX NCBI_TaxID=28377 {ECO:0000313|Ensembl:ENSACAP00000008110.4, ECO:0000313|Proteomes:UP000001646};
RN [1] {ECO:0000313|Ensembl:ENSACAP00000008110.4, ECO:0000313|Proteomes:UP000001646}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JBL SC #1 {ECO:0000313|Ensembl:ENSACAP00000008110.4,
RC ECO:0000313|Proteomes:UP000001646};
RG The Genome Sequencing Platform;
RA Di Palma F., Alfoldi J., Heiman D., Young S., Grabherr M., Johnson J.,
RA Lander E.S., Lindblad-Toh K.;
RT "The Genome Sequence of Anolis carolinensis (Green Anole Lizard).";
RL Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSACAP00000008110.4}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=RNA(n) + UTP = diphosphate + RNA(n)-3'-uridine ribonucleotide;
CC Xref=Rhea:RHEA:14785, Rhea:RHEA-COMP:14527, Rhea:RHEA-COMP:17348,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:46398, ChEBI:CHEBI:140395,
CC ChEBI:CHEBI:173116; EC=2.7.7.52;
CC Evidence={ECO:0000256|ARBA:ARBA00024498};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- COFACTOR:
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000256|ARBA:ARBA00001936};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
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DR STRING; 28377.ENSACAP00000008110; -.
DR Ensembl; ENSACAT00000008282.4; ENSACAP00000008110.4; ENSACAG00000008282.4.
DR eggNOG; KOG2277; Eukaryota.
DR GeneTree; ENSGT00940000156988; -.
DR HOGENOM; CLU_003287_0_0_1; -.
DR InParanoid; G1KHK6; -.
DR OrthoDB; 170176at2759; -.
DR TreeFam; TF315661; -.
DR Proteomes; UP000001646; Chromosome 4.
DR Bgee; ENSACAG00000008282; Expressed in embryonic post-anal tail and 13 other cell types or tissues.
DR GO; GO:0005829; C:cytosol; IEA:Ensembl.
DR GO; GO:0005730; C:nucleolus; IEA:Ensembl.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0050265; F:RNA uridylyltransferase activity; IBA:GO_Central.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0006402; P:mRNA catabolic process; IEA:UniProt.
DR GO; GO:0061157; P:mRNA destabilization; IEA:UniProt.
DR CDD; cd05402; NT_PAP_TUTase; 2.
DR Gene3D; 1.10.1410.10; -; 2.
DR Gene3D; 3.30.460.10; Beta Polymerase, domain 2; 1.
DR Gene3D; 4.10.60.10; Zinc finger, CCHC-type; 1.
DR InterPro; IPR043519; NT_sf.
DR InterPro; IPR002058; PAP_assoc.
DR InterPro; IPR002934; Polymerase_NTP_transf_dom.
DR InterPro; IPR045100; TUTase_dom.
DR InterPro; IPR001878; Znf_CCHC.
DR InterPro; IPR036875; Znf_CCHC_sf.
DR PANTHER; PTHR12271; POLY A POLYMERASE CID PAP -RELATED; 1.
DR PANTHER; PTHR12271:SF49; TERMINAL URIDYLYLTRANSFERASE 4; 1.
DR Pfam; PF01909; NTP_transf_2; 1.
DR Pfam; PF03828; PAP_assoc; 2.
DR Pfam; PF19088; TUTase; 2.
DR Pfam; PF00098; zf-CCHC; 2.
DR SMART; SM00343; ZnF_C2HC; 3.
DR SUPFAM; SSF81301; Nucleotidyltransferase; 2.
DR SUPFAM; SSF81631; PAP/OAS1 substrate-binding domain; 2.
DR SUPFAM; SSF57756; Retrovirus zinc finger-like domains; 2.
DR PROSITE; PS50158; ZF_CCHC; 2.
PE 4: Predicted;
KW Metal-binding {ECO:0000256|PROSITE-ProRule:PRU00047};
KW Reference proteome {ECO:0000313|Proteomes:UP000001646};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Zinc {ECO:0000256|PROSITE-ProRule:PRU00047};
KW Zinc-finger {ECO:0000256|PROSITE-ProRule:PRU00047}.
FT DOMAIN 1211..1226
FT /note="CCHC-type"
FT /evidence="ECO:0000259|PROSITE:PS50158"
FT DOMAIN 1254..1270
FT /note="CCHC-type"
FT /evidence="ECO:0000259|PROSITE:PS50158"
FT REGION 1..182
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 486..530
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 626..659
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1294..1357
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..23
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 32..143
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 161..175
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 627..645
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1530 AA; 172369 MW; FE0B0BDE94533453 CRC64;
MEEGKSSKSG NHDTRKSVHT ACEKSKAVRV ATNPVYKTNK NDRSGKSSSS GSSLKKNKQN
AVSPEKSGEN KSCKTDSCTP GCTKMGSSVQ DQSRSKAERL LNVSETTVNL HQLKNQHTDQ
NPPSSYVSEN GAITETLPIA QRESSELKNL PGEDISKINR SGDSGKSNTK PPDSSKETNS
EAKCLENVAV TGESTLIAEQ ELGLKQAEER LERDYIIRLE KRSPEYANCQ YLCKLCLVHI
ENIQGAHKHI KEKRHKKNIM EKQEENELRA LPPPSPAQVA ALSFMLMEAA KEQGISDDDF
KIRQDIVRDM EKIIQQHLPE CTLRMYGSCL TRFAFKTNIS CYQTEVFYIS CYSDVESDFH
AKVPVIFCKD NKSGLTCKVS AGNDVACLTT DLLAALGKLE PVLVPLVLAF RYWARLCHID
CQAEGGIPSY SFALMVIFFL QQREPRILPS YLGSWIEGFD SKKADDYQLK RIKDEKFVVW
EYKPSNSGGK NVSAAEGKAK GEQHSGDKNA SSVETDSQHN AKEKNGSSPL ALEEPHQISL
GHLWLELLKF YTLEFALEEY VISIRVQELL TRENKTWPKR RIAIEDPFAL KRNVARSLNS
QMVFEYILER FRTAYKYFAC PQNKDGSKLK SDTRKKEKGK FGSKKPEEPA ANCSLPQQAN
PAEKQSAGCA DNILECELNE CSEMEPTSRK RTSENSKSLL AKTPEILNTN CDSREETVSL
IANECCELEH KSLEAKNDQE TTTEENRVFS EHRSFDADFV SELTDTENRQ NSIIEYPQIN
MCSGTSTSAT SHNCQAVGEI QDTNDEIATE DMHYVFDKFI LTSGKPPTIV CSICKRDGHS
KNDCPEDFKK IDLKPLPPMT SRFREILDLV CKRCFDELSP PFSEQQNREQ ILASLERFIR
KEYNDKARLC LFGSSKNGFG FRDSDLDICM TLEGHENAEK LNCKEIIENL AKVLKKHPGL
RNILPITTAK VPIVKFEHRR SGLEGDISLY NTLAQHNTRM LATYAAIDPR VQYLGYTMKV
FAKRCDIGDA SRGSLSSYAY ILMVLYFLQQ REPPVIPVLQ EIFDGQQIPQ RMVDGWNAFF
FDDTEELKKR LPSLGKNTES LGELWLGLLR FYTEEFDFKE YVISIRQKKL LTTFEKQWTS
KCIAIEDPFD LNHNLGAGVS RKMTNFIMKA LINGRKLFGT PFYPVLGREA EYFFNSKVLT
DGELAPNDRC CRVCGKIGHY MKDCPKRRRL KKKENEREDE KEVKEEDRDT REKRCFICGD
VGHVRRDCPE FKQTRQRSNA AQLVRSLVNA QPVTGSIPQQ VERPLRTRQP SECSDSHPSS
YSPQPQQFTQ NSSPPASINQ TQPQPIAPSN HVLQPQPGTQ VQLPLFNFAQ SPPGQYPNLP
NLGLFQMHHH QIQLPNTSWP VIHSAPGNPS HSSLSLNDPS IIFAQPAARP VGIPSNSPEG
HWHNSVTLSS LVNNGTVGNA GQFGKTNPSP AAAAAPWDHG SPAHFPLLRG TWPYSMSPNY
VQQGNTFPSP LYILLIHCSI LRCKKKLLCS
//
