ID G3ARW0_SPAPN Unreviewed; 918 AA.
AC G3ARW0;
DT 16-NOV-2011, integrated into UniProtKB/TrEMBL.
DT 16-NOV-2011, sequence version 1.
DT 27-MAR-2024, entry version 60.
DE RecName: Full=ATP-dependent RNA helicase DBP10 {ECO:0000256|ARBA:ARBA00019117};
DE EC=3.6.4.13 {ECO:0000256|ARBA:ARBA00012552};
DE AltName: Full=ATP-dependent RNA helicase dbp10 {ECO:0000256|ARBA:ARBA00021760};
GN ORFNames=SPAPADRAFT_140104 {ECO:0000313|EMBL:EGW31377.1};
OS Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Spathaspora.
OX NCBI_TaxID=619300 {ECO:0000313|Proteomes:UP000000709};
RN [1] {ECO:0000313|EMBL:EGW31377.1, ECO:0000313|Proteomes:UP000000709}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NRRL Y-27907 / 11-Y1 {ECO:0000313|Proteomes:UP000000709};
RX PubMed=21788494; DOI=10.1073/pnas.1103039108;
RA Wohlbach D.J., Kuo A., Sato T.K., Potts K.M., Salamov A.A., LaButti K.M.,
RA Sun H., Clum A., Pangilinan J.L., Lindquist E.A., Lucas S., Lapidus A.,
RA Jin M., Gunawan C., Balan V., Dale B.E., Jeffries T.W., Zinkel R.,
RA Barry K.W., Grigoriev I.V., Gasch A.P.;
RT "Comparative genomics of xylose-fermenting fungi for enhanced biofuel
RT production.";
RL Proc. Natl. Acad. Sci. U.S.A. 108:13212-13217(2011).
CC -!- FUNCTION: ATP-binding RNA helicase involved in the biogenesis of 60S
CC ribosomal subunits and is required for the normal formation of 25S and
CC 5.8S rRNAs. {ECO:0000256|ARBA:ARBA00003706}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC Evidence={ECO:0000256|ARBA:ARBA00001556};
CC -!- SIMILARITY: Belongs to the DEAD box helicase family. DDX54/DBP10
CC subfamily. {ECO:0000256|ARBA:ARBA00010379}.
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DR EMBL; GL996503; EGW31377.1; -; Genomic_DNA.
DR RefSeq; XP_007376155.1; XM_007376093.1.
DR AlphaFoldDB; G3ARW0; -.
DR STRING; 619300.G3ARW0; -.
DR GeneID; 18870268; -.
DR KEGG; spaa:SPAPADRAFT_140104; -.
DR eggNOG; KOG0337; Eukaryota.
DR HOGENOM; CLU_003041_5_1_1; -.
DR InParanoid; G3ARW0; -.
DR OMA; EDQFGMM; -.
DR OrthoDB; 5475716at2759; -.
DR Proteomes; UP000000709; Unassembled WGS sequence.
DR GO; GO:0005634; C:nucleus; IEA:InterPro.
DR GO; GO:0030684; C:preribosome; IEA:UniProt.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0006364; P:rRNA processing; IEA:UniProt.
DR CDD; cd17959; DEADc_DDX54; 1.
DR CDD; cd18787; SF2_C_DEAD; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR012541; DBP10_C.
DR InterPro; IPR033517; DDX54/DBP10_DEAD-box_helicase.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR000629; RNA-helicase_DEAD-box_CS.
DR InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR PANTHER; PTHR47959; ATP-DEPENDENT RNA HELICASE RHLE-RELATED; 1.
DR PANTHER; PTHR47959:SF8; RNA HELICASE; 1.
DR Pfam; PF08147; DBP10CT; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR SMART; SM01123; DBP10CT; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 2.
DR PROSITE; PS00039; DEAD_ATP_HELICASE; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
DR PROSITE; PS51195; Q_MOTIF; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000313|EMBL:EGW31377.1};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Reference proteome {ECO:0000313|Proteomes:UP000000709};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884}.
FT DOMAIN 102..130
FT /note="DEAD-box RNA helicase Q"
FT /evidence="ECO:0000259|PROSITE:PS51195"
FT DOMAIN 133..304
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 371..531
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
FT REGION 1..84
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 354..373
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 826..918
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 102..130
FT /note="Q motif"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00552"
FT COMPBIAS 20..44
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 45..61
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 838..877
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 903..918
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 918 AA; 105018 MW; 8761ADC966463773 CRC64;
MSDDEDFDIA GSLGLNQEEE EDYSSSDNES EQEIQDEIIS SDEEEQADEP PKKKTKPTKK
QESFPSLELS DNEQDREDAD DDSQLNSYFI ANNPATKKAK AGSFASFGLS KFLLTNIAKK
GFRQPTPIQR KTIPLVMENR DVVGMARTGS GKTAAFVLPL IEKLKVRSAS GVRAIILSPS
RELALQTYKQ VKEFGHGTNL QTTVLIGGDT LEEDFSKMVS KPDIIVCTPG RFLHLKVEMD
YDLSTIQYIV FDEADRLFEM GFAIQLNELL ASLPPNRQSL LFSATLPRSL VDFAKAGLTN
PVLVRLDAET KISDQLQMAY FTTKKNEREA NLLYILQEVI KLPLGSPEQI KKLKDMDKRQ
VESDEENEED GKKKKYKFKK ERMPAANQLP SEFSTIVFVP TKHHVEYVTR MLRDAGYLVS
YIYGTLDQHA RKNQLYQFRI GLTHIMVVTD VAARGIDIPV LANVINFTLP GSSKIFIHRV
GRTARAGNKG WAYSIVNEKE LPYLLDLELF LGKKILLTSM HETKCEVLKR KQGDAYVPPK
VNYTERLVLG SIPRLDLEGC QELYENLLRN DYELKVLRDV AAKGEKLYHR TRQAASPESL
KRSKEIMETG SWDEQHVLFG PNLEKQKQDF LAKLQNRNVK ETVFEFNKKG NDKDENSLVE
FMHRRRRQIA PIQRRAKERR ELLQKERNAG LTHGIEDEIL HEEGETGYNA DEQELEQVFE
DADKISSKKK TYRDPNFFLS HYAPASVIQD QQLSISSSFA NDAQAATFDL DNDDKIQKNK
QTMAWDKKKG KYVNSKSTDV KYIIGESGQK IPATFRSGKF DEWKKKRGAN LTTESTGESD
NKRFKHKKVQ TPKLPDKFRD DYHKQKKKVE KAVEGGMKVR GYNKPGHQQE IKSTEQIRKA
RMVKEQRRAK NARPSRRK
//
