ID G3H184_CRIGR Unreviewed; 867 AA.
AC G3H184;
DT 16-NOV-2011, integrated into UniProtKB/TrEMBL.
DT 16-NOV-2011, sequence version 1.
DT 24-JAN-2024, entry version 56.
DE RecName: Full=hexokinase {ECO:0000256|ARBA:ARBA00012324};
DE EC=2.7.1.1 {ECO:0000256|ARBA:ARBA00012324};
GN ORFNames=I79_003908 {ECO:0000313|EMBL:EGV96489.1};
OS Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea;
OC Cricetidae; Cricetinae; Cricetulus.
OX NCBI_TaxID=10029 {ECO:0000313|EMBL:EGV96489.1, ECO:0000313|Proteomes:UP000001075};
RN [1] {ECO:0000313|Proteomes:UP000001075}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CHO K1 cell line {ECO:0000313|Proteomes:UP000001075};
RX PubMed=21804562; DOI=10.1038/nbt.1932;
RA Xu X., Nagarajan H., Lewis N.E., Pan S., Cai Z., Liu X., Chen W., Xie M.,
RA Wang W., Hammond S., Andersen M.R., Neff N., Passarelli B., Koh W.,
RA Fan H.C., Wang J., Gui Y., Lee K.H., Betenbaugh M.J., Quake S.R.,
RA Famili I., Palsson B.O., Wang J.;
RT "The genomic sequence of the Chinese hamster ovary (CHO)-K1 cell line.";
RL Nat. Biotechnol. 29:735-741(2011).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + D-glucose = ADP + D-glucose 6-phosphate + H(+);
CC Xref=Rhea:RHEA:17825, ChEBI:CHEBI:4167, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61548, ChEBI:CHEBI:456216; EC=2.7.1.1;
CC Evidence={ECO:0000256|ARBA:ARBA00000435};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:17826;
CC Evidence={ECO:0000256|ARBA:ARBA00000435};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + D-hexose = ADP + D-hexose 6-phosphate + H(+);
CC Xref=Rhea:RHEA:22740, ChEBI:CHEBI:4194, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61567, ChEBI:CHEBI:456216; EC=2.7.1.1;
CC Evidence={ECO:0000256|ARBA:ARBA00000417};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:22741;
CC Evidence={ECO:0000256|ARBA:ARBA00000417};
CC -!- PATHWAY: Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-
CC phosphate and glycerone phosphate from D-glucose: step 1/4.
CC {ECO:0000256|ARBA:ARBA00004888}.
CC -!- PATHWAY: Carbohydrate metabolism; hexose metabolism.
CC {ECO:0000256|ARBA:ARBA00005028}.
CC -!- SIMILARITY: Belongs to the hexokinase family.
CC {ECO:0000256|ARBA:ARBA00009225}.
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DR EMBL; JH000102; EGV96489.1; -; Genomic_DNA.
DR AlphaFoldDB; G3H184; -.
DR STRING; 10029.G3H184; -.
DR PaxDb; 10029-XP_007613622-1; -.
DR eggNOG; KOG1369; Eukaryota.
DR InParanoid; G3H184; -.
DR OMA; VRPCEVG; -.
DR UniPathway; UPA00109; UER00180.
DR UniPathway; UPA00242; -.
DR Proteomes; UP000001075; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0005536; F:glucose binding; IEA:InterPro.
DR GO; GO:0004396; F:hexokinase activity; IEA:UniProtKB-EC.
DR GO; GO:0006096; P:glycolytic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0019318; P:hexose metabolic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0001678; P:intracellular glucose homeostasis; IEA:InterPro.
DR CDD; cd00012; NBD_sugar-kinase_HSP70_actin; 2.
DR Gene3D; 3.30.420.40; -; 2.
DR Gene3D; 3.40.367.20; -; 2.
DR InterPro; IPR043129; ATPase_NBD.
DR InterPro; IPR001312; Hexokinase.
DR InterPro; IPR019807; Hexokinase_BS.
DR InterPro; IPR022673; Hexokinase_C.
DR InterPro; IPR022672; Hexokinase_N.
DR PANTHER; PTHR19443; HEXOKINASE; 1.
DR PANTHER; PTHR19443:SF1; HEXOKINASE-3; 1.
DR Pfam; PF00349; Hexokinase_1; 2.
DR Pfam; PF03727; Hexokinase_2; 2.
DR PRINTS; PR00475; HEXOKINASE.
DR SUPFAM; SSF53067; Actin-like ATPase domain; 4.
DR PROSITE; PS00378; HEXOKINASE_1; 2.
DR PROSITE; PS51748; HEXOKINASE_2; 2.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Glycolysis {ECO:0000256|ARBA:ARBA00023152};
KW Kinase {ECO:0000256|ARBA:ARBA00022777, ECO:0000313|EMBL:EGV96489.1};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Reference proteome {ECO:0000313|Proteomes:UP000001075};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 1..174
FT /note="Hexokinase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF00349"
FT DOMAIN 182..415
FT /note="Hexokinase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF03727"
FT DOMAIN 426..617
FT /note="Hexokinase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF00349"
FT DOMAIN 623..857
FT /note="Hexokinase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF03727"
SQ SEQUENCE 867 AA; 93912 MW; 5F0338520ABE074A CRC64;
MEQALKGHDS PAPSVRMLPT YVGSTPHGTE QGDFLVLELG ATGASLRVLW VTLTGTKECS
VEPKSQEFAI PREVMLGAGR QLFDFAARCL SEFLDAHPTE NQDLKLGFNF SFPCHQTGLD
RSTLISWTKG FRCSGVEGQD VVQLLREAIQ RQGTYSIDVV AMVNDTVGTM MGCELGTRPC
EVGLIADTGT NACYMEEARH VRALDEDRGR TCVSIEWGSF YDAEALGPVL STFDHALDHD
SLTPGAQRFE KMIGGLYLGE LVRLVLVHLA QHGVLFGGCT SPALLSQGSI LLEHVAEIED
PTTGTAHAHA ILQDLGLSPQ ASDAELVKHV CAAVCTRAAQ LCAAALAAVL SRLQHSREQQ
TLQVAVATGG RVFERHPRFR HTLKEMVMLL VPECDVSFIP SVDGGGRGVA MVTAVAARLA
AHKHILEETL APFQLDFEQL TVVQAQMREA MVRGLRGEAS SLRMLPTYVR ATPDGSERGD
FLALDLGGTN FRVLLVRVAE GSVQITNQVY SIPESVTQGS GQQLFDHIVD CIVDFQQRHG
LSGQSLPLGF TFSFPCKQLG LDQGILLNWT KGFNASDCEG QDVVYLLREA IRRKQAVELN
VVAIVNDTVG TMMSCGYDDP RCEMGLIVGT GTNACYMEEL RNVAGVAGDS GRMCINMEWG
AFGDDGSLGL LSTCFDASVD QASINPGKQR FEKMISGMYL GEIVRHILLH LTSLGVLFRG
QKTQCLQTRD IFKTKFLSVI ESDSLALRQV RAILEDLGLT LTSDDALMVL EVCQAVSRRA
AQLCGAGVAA VVEKIRENRG LQELSVSVGV DGTLYKLHPH FSRLVSATVR KLAPHCTVTF
LQSEDGSGKG AALVTAVACR LVQRARD
//
