ID G3PFM2_GASAC Unreviewed; 387 AA.
AC G3PFM2;
DT 16-NOV-2011, integrated into UniProtKB/TrEMBL.
DT 16-NOV-2011, sequence version 1.
DT 27-MAR-2024, entry version 66.
DE RecName: Full=Gastricsin {ECO:0000256|ARBA:ARBA00023821};
DE EC=3.4.23.3 {ECO:0000256|ARBA:ARBA00023796};
OS Gasterosteus aculeatus (Three-spined stickleback).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Eupercaria; Perciformes; Cottioidei; Gasterosteales; Gasterosteidae;
OC Gasterosteus.
OX NCBI_TaxID=69293 {ECO:0000313|Ensembl:ENSGACP00000016396.1, ECO:0000313|Proteomes:UP000007635};
RN [1] {ECO:0000313|Ensembl:ENSGACP00000016396.1, ECO:0000313|Proteomes:UP000007635}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Lindblad-Toh K., Mauceli E., Grabherr M., Chang J.L., Lander E.S.;
RL Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSGACP00000016396.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- FUNCTION: Hydrolyzes a variety of proteins.
CC {ECO:0000256|ARBA:ARBA00023749}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=More restricted specificity than pepsin A, but shows
CC preferential cleavage at Tyr-|-Xaa bonds. High activity on
CC hemoglobin.; EC=3.4.23.3; Evidence={ECO:0000256|ARBA:ARBA00023733};
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000256|ARBA:ARBA00004613}.
CC -!- SIMILARITY: Belongs to the peptidase A1 family.
CC {ECO:0000256|ARBA:ARBA00007447, ECO:0000256|RuleBase:RU000454}.
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DR AlphaFoldDB; G3PFM2; -.
DR STRING; 69293.ENSGACP00000016396; -.
DR Ensembl; ENSGACT00000016428.1; ENSGACP00000016396.1; ENSGACG00000012388.1.
DR eggNOG; KOG1339; Eukaryota.
DR GeneTree; ENSGT00940000164965; -.
DR InParanoid; G3PFM2; -.
DR OMA; THATWDE; -.
DR TreeFam; TF314990; -.
DR Proteomes; UP000007635; Unassembled WGS sequence.
DR Bgee; ENSGACG00000012388; Expressed in liver.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0004190; F:aspartic-type endopeptidase activity; IEA:UniProtKB-KW.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR Gene3D; 6.10.140.60; -; 1.
DR Gene3D; 2.40.70.10; Acid Proteases; 2.
DR InterPro; IPR001461; Aspartic_peptidase_A1.
DR InterPro; IPR001969; Aspartic_peptidase_AS.
DR InterPro; IPR012848; Aspartic_peptidase_N.
DR InterPro; IPR033121; PEPTIDASE_A1.
DR InterPro; IPR021109; Peptidase_aspartic_dom_sf.
DR PANTHER; PTHR47966; BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED; 1.
DR PANTHER; PTHR47966:SF72; GASTRICSIN; 1.
DR Pfam; PF07966; A1_Propeptide; 1.
DR Pfam; PF00026; Asp; 1.
DR PRINTS; PR00792; PEPSIN.
DR SUPFAM; SSF50630; Acid proteases; 1.
DR PROSITE; PS00141; ASP_PROTEASE; 1.
DR PROSITE; PS51767; PEPTIDASE_A1; 1.
PE 3: Inferred from homology;
KW Aspartyl protease {ECO:0000256|RuleBase:RU000454};
KW Disulfide bond {ECO:0000256|ARBA:ARBA00023157,
KW ECO:0000256|PIRSR:PIRSR601461-2};
KW Hydrolase {ECO:0000256|RuleBase:RU000454};
KW Protease {ECO:0000256|RuleBase:RU000454};
KW Reference proteome {ECO:0000313|Proteomes:UP000007635};
KW Secreted {ECO:0000256|ARBA:ARBA00022525};
KW Signal {ECO:0000256|ARBA:ARBA00022729, ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..16
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 17..387
FT /note="Gastricsin"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5003449556"
FT DOMAIN 69..384
FT /note="Peptidase A1"
FT /evidence="ECO:0000259|PROSITE:PS51767"
FT ACT_SITE 87
FT /evidence="ECO:0000256|PIRSR:PIRSR601461-1"
FT ACT_SITE 271
FT /evidence="ECO:0000256|PIRSR:PIRSR601461-1"
FT DISULFID 100..105
FT /evidence="ECO:0000256|PIRSR:PIRSR601461-2"
SQ SEQUENCE 387 AA; 42093 MW; B792A0B072A2A2AB CRC64;
MKCLVAVLVC VVLAEGIVKI PLQKHKSMRE ALRDKGIELP YQDPALKYQA DEFAGSASMY
INNYADTTYY GAISIGTPPQ SFQVLFDTGS ANLWVDSVYC NTQACNTHTK FNPQQSSTYS
GKGQTFYLPY GAGSLYGTFG YDTVNVGGIV INNQEIGLST NEPGQNFVVA KFDGILGLSY
PSISAGGETP VMDNMISQNL LSADMFAFYL SRGGQQGSVL SFGGVDNSLY QGQIYWTPVT
SQTYWQIGVN GFQINGQETG WCSQGCQSIV DTGTSALTAP SQFLGSIMQA IGAQQNQYGQ
YMVDCSQINN LPTFSFVISG VSLPLPPSAY ITQTNQNGYQ YCSVDISPTY LPSQDGQPLW
IMGDVFLREY YSVYDRSNNR LGFAAAV
//
