ID G3Q5U9_GASAC Unreviewed; 855 AA.
AC G3Q5U9;
DT 16-NOV-2011, integrated into UniProtKB/TrEMBL.
DT 16-NOV-2011, sequence version 1.
DT 27-MAR-2024, entry version 55.
DE RecName: Full=Non-lysosomal glucosylceramidase {ECO:0000256|PIRNR:PIRNR028944};
DE Short=NLGase {ECO:0000256|PIRNR:PIRNR028944};
DE EC=3.2.1.45 {ECO:0000256|PIRNR:PIRNR028944};
GN Name=GBA2 {ECO:0000313|Ensembl:ENSGACP00000025255.1};
OS Gasterosteus aculeatus (Three-spined stickleback).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Eupercaria; Perciformes; Cottioidei; Gasterosteales; Gasterosteidae;
OC Gasterosteus.
OX NCBI_TaxID=69293 {ECO:0000313|Ensembl:ENSGACP00000025255.1, ECO:0000313|Proteomes:UP000007635};
RN [1] {ECO:0000313|Ensembl:ENSGACP00000025255.1, ECO:0000313|Proteomes:UP000007635}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Lindblad-Toh K., Mauceli E., Grabherr M., Chang J.L., Lander E.S.;
RL Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSGACP00000025255.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- FUNCTION: Non-lysosomal glucosylceramidase that catalyzes the
CC hydrolysis of glucosylceramide (GlcCer) to free glucose and ceramide.
CC {ECO:0000256|PIRNR:PIRNR028944}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-
CC acylsphing-4-enine + D-glucose; Xref=Rhea:RHEA:13269,
CC ChEBI:CHEBI:4167, ChEBI:CHEBI:15377, ChEBI:CHEBI:22801,
CC ChEBI:CHEBI:52639; EC=3.2.1.45;
CC Evidence={ECO:0000256|PIRNR:PIRNR028944};
CC -!- SIMILARITY: Belongs to the non-lysosomal glucosylceramidase family.
CC {ECO:0000256|PIRNR:PIRNR028944}.
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DR AlphaFoldDB; G3Q5U9; -.
DR STRING; 69293.ENSGACP00000025255; -.
DR Ensembl; ENSGACT00000025304.1; ENSGACP00000025255.1; ENSGACG00000019099.1.
DR eggNOG; KOG2119; Eukaryota.
DR GeneTree; ENSGT00390000010998; -.
DR InParanoid; G3Q5U9; -.
DR OMA; HDLGAPN; -.
DR TreeFam; TF313888; -.
DR Proteomes; UP000007635; Unassembled WGS sequence.
DR Bgee; ENSGACG00000019099; Expressed in intestinal epithelial cell and 12 other cell types or tissues.
DR GO; GO:0005886; C:plasma membrane; IEA:Ensembl.
DR GO; GO:0008422; F:beta-glucosidase activity; IEA:Ensembl.
DR GO; GO:0004348; F:glucosylceramidase activity; IEA:UniProtKB-EC.
DR GO; GO:0007409; P:axonogenesis; IEA:Ensembl.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR GO; GO:0007417; P:central nervous system development; IEA:Ensembl.
DR GO; GO:0006680; P:glucosylceramide catabolic process; IEA:InterPro.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR014551; B_Glucosidase_GBA2-typ.
DR InterPro; IPR006775; GH116_catalytic.
DR InterPro; IPR024462; GH116_N.
DR PANTHER; PTHR12654; BILE ACID BETA-GLUCOSIDASE-RELATED; 1.
DR PANTHER; PTHR12654:SF0; NON-LYSOSOMAL GLUCOSYLCERAMIDASE; 1.
DR Pfam; PF04685; DUF608; 1.
DR Pfam; PF12215; Glyco_hydr_116N; 1.
DR PIRSF; PIRSF028944; Beta_gluc_GBA2; 1.
DR SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|PIRNR:PIRNR028944};
KW Hydrolase {ECO:0000256|PIRNR:PIRNR028944};
KW Lipid metabolism {ECO:0000256|PIRNR:PIRNR028944};
KW Membrane {ECO:0000256|PIRNR:PIRNR028944};
KW Reference proteome {ECO:0000313|Proteomes:UP000007635}.
FT DOMAIN 89..392
FT /note="Glycosyl-hydrolase family 116 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF12215"
FT DOMAIN 458..823
FT /note="Glycosyl-hydrolase family 116 catalytic region"
FT /evidence="ECO:0000259|Pfam:PF04685"
FT REGION 832..855
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 838..855
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 855 AA; 96381 MW; 90EAF1A351DE7928 CRC64;
KSSADLMSRY VSKEMGCGVP KDGWRICLAH EFKEKRKPFQ AKDVSLSNVL EHVGLGFRYL
KWWYKKTQVE KKAPFIDMFR AQPLRQIYGA PLGGIGGGTI TRGWRGEFCR WQLNPGMYHY
KTVTANQFTV CLRRGGRTVY QQVLSVERPP TLQGWNWGYC GEYAFYHALY PRAWTVYHLP
GQNVTLTCRQ ISPVIPHDYQ VTRASPVAVF VWDVENNNDD ALDVSIMFTV VNGSGHKDDK
SGGHWNEPFH LEKEGEAVSG VLLHHCTSVN PYTLCVAARE QPDREVSHQT AFSPKGTCSG
LWSDLIADGR LDSPTGSSPP TPKGEKVAAA LAVGCSVAAR SRNCLEFCLA WDMPTITFGS
REREHIRRYT RYFGSKGDAS PSLGHHALTH YRARRFKSSV SDADSLPSWY KSALFNELYF
VVDGTVWTEL PEDADVSGGV RSEDGGLPAQ PAVIKEFGRF AYLEGQEYRM YNTYDVHFYA
SFALIMLWPK LALSLQYDIA GSVIQQDPTE RLHLMSGRCS PVKAKNVVPH DIGDPDDEPW
QRVNAYLIHD TADWKDLNLK FVLQVYRDFH LTQDSLYLRE MWPVCQAVME SEMKFDLDGD
GLIENSGYAD QTYDGWTVTG PSAYCGGLWL ASLCVMCKMA QLVDSEETHR RYRDILDRGR
AAFDKLLWNG KYYNYDSSGR DLSNSVMSDQ CAGHWFLRAS GLGEGDYKAF PKEKIQSALK
SVFDLNVMSF AGGQMGAVNG MRPEGVPDRS SVQSDEVWIG VVYGLAATMI HEGMKEEGMR
TAEGCYRTVW ERLGMAFQTP EAYCEKGIYR SLAYMRPLSV WAMQLALNSS QKDQATSARA
RATDEKQEPD LREES
//
