ID   G3ST4_BOVIN             Reviewed;         483 AA.
AC   Q5E9W5; Q0VCZ6;
DT   07-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 1.
DT   08-NOV-2023, entry version 85.
DE   RecName: Full=Galactose-3-O-sulfotransferase 4;
DE            Short=Gal3ST-4;
DE            EC=2.8.2.-;
DE   AltName: Full=Beta-galactose-3-O-sulfotransferase 4;
DE   AltName: Full=Gal-beta-1,3-GalNAc 3'-sulfotransferase;
GN   Name=GAL3ST4;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX   PubMed=16305752; DOI=10.1186/1471-2164-6-166;
RA   Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L.,
RA   Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L.;
RT   "Characterization of 954 bovine full-CDS cDNA sequences.";
RL   BMC Genomics 6:166-166(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Fetal skin;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the transfer of sulfate to beta-1,3-linked
CC       galactose residues in O-linked glycoproteins. Good substrates include
CC       asialofetuin, Gal-beta-1,3-GalNAc and Gal-beta-1,3 (GlcNAc-beta-
CC       1,6)GalNAc (By similarity). {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC   -!- PATHWAY: Protein modification; carbohydrate sulfation.
CC   -!- SUBCELLULAR LOCATION: Golgi apparatus, Golgi stack membrane
CC       {ECO:0000305}; Single-pass type II membrane protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the galactose-3-O-sulfotransferase family.
CC       {ECO:0000305}.
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DR   EMBL; BT020805; AAX08822.1; -; mRNA.
DR   EMBL; BC119916; AAI19917.1; -; mRNA.
DR   RefSeq; NP_001029709.1; NM_001034537.1.
DR   AlphaFoldDB; Q5E9W5; -.
DR   STRING; 9913.ENSBTAP00000019991; -.
DR   GlyCosmos; Q5E9W5; 1 site, No reported glycans.
DR   PaxDb; 9913-ENSBTAP00000019991; -.
DR   GeneID; 520552; -.
DR   KEGG; bta:520552; -.
DR   CTD; 79690; -.
DR   eggNOG; ENOG502QSHR; Eukaryota.
DR   InParanoid; Q5E9W5; -.
DR   OrthoDB; 3032344at2759; -.
DR   UniPathway; UPA00353; -.
DR   Proteomes; UP000009136; Unplaced.
DR   GO; GO:0032580; C:Golgi cisterna membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0050694; F:galactose 3-O-sulfotransferase activity; IBA:GO_Central.
DR   GO; GO:0001733; F:galactosylceramide sulfotransferase activity; IEA:InterPro.
DR   GO; GO:0009247; P:glycolipid biosynthetic process; IEA:InterPro.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR   InterPro; IPR009729; Gal-3-0_sulfotransfrase.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR14647; GALACTOSE-3-O-SULFOTRANSFERASE; 1.
DR   PANTHER; PTHR14647:SF57; GALACTOSE-3-O-SULFOTRANSFERASE 4; 1.
DR   Pfam; PF06990; Gal-3-0_sulfotr; 2.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
PE   2: Evidence at transcript level;
KW   Glycoprotein; Golgi apparatus; Membrane; Reference proteome; Signal-anchor;
KW   Transferase; Transmembrane; Transmembrane helix.
FT   CHAIN           1..483
FT                   /note="Galactose-3-O-sulfotransferase 4"
FT                   /id="PRO_0000085208"
FT   TOPO_DOM        1..18
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        19..39
FT                   /note="Helical; Signal-anchor for type II membrane protein"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        40..483
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   REGION          225..248
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CARBOHYD        371
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   483 AA;  53830 MW;  6C7EA2B051132101 CRC64;
     MGVLSPTRTM RLWGPRSLGV ALGVFMTIGF ALQLLGGPFQ RRLPGLQLRH SWASSLGPAA
     PSCPPRQHLV FLKTHKSGSS SVLSLLHRYG DRHGLRFALP ARYQFGYPRL FQASRVKGYR
     PQSGGTQPPF HILCHHMRFN LKEVLQVMPS DSFFFSIVRD PAALARSAFS YYKSTSSAFR
     KAPSLAAFLA NPRAFYRPGA RGDHYARNLL WFDFGLPFPP ELRTKRGNPH VSRDPNPPQL
     PSGAGPPAHT LDPNALFHPV PIVADGHSQM SSPASLDLGS SSFIQWNLAW LDSVFDLVLV
     AEYFDESLVL LADALCWGLD DVVGFMHNAQ AGGGQDRSTI DDGGLTTEER QLTARARAWN
     NLDWALYVHF NRSLWARIKQ YGQSRLDSAV AELRARREAL AKHCLVGGEA LDPKYITDRR
     FRPFQFGSGK VLGYVLRSGL SLQDQEECER LATPELQYKD KLDAKQFPPT VSLPLKTSRR
     PSP
//