ID G3U5A3_LOXAF Unreviewed; 1243 AA.
AC G3U5A3;
DT 16-NOV-2011, integrated into UniProtKB/TrEMBL.
DT 16-NOV-2011, sequence version 1.
DT 27-MAR-2024, entry version 62.
DE RecName: Full=HECT-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012485};
DE EC=2.3.2.26 {ECO:0000256|ARBA:ARBA00012485};
GN Name=HECW2 {ECO:0000313|Ensembl:ENSLAFP00000023011.1};
OS Loxodonta africana (African elephant).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Afrotheria; Proboscidea; Elephantidae; Loxodonta.
OX NCBI_TaxID=9785 {ECO:0000313|Ensembl:ENSLAFP00000023011.1, ECO:0000313|Proteomes:UP000007646};
RN [1] {ECO:0000313|Ensembl:ENSLAFP00000023011.1, ECO:0000313|Proteomes:UP000007646}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Isolate ISIS603380 {ECO:0000313|Ensembl:ENSLAFP00000023011.1,
RC ECO:0000313|Proteomes:UP000007646};
RA Di Palma F., Heiman D., Young S., Johnson J., Lander E.S., Lindblad-Toh K.;
RT "The Genome Sequence of Loxodonta africana (African elephant).";
RL Submitted (JUN-2009) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSLAFP00000023011.1}
RP IDENTIFICATION.
RC STRAIN=Isolate ISIS603380 {ECO:0000313|Ensembl:ENSLAFP00000023011.1};
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.26; Evidence={ECO:0000256|ARBA:ARBA00000885};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000256|ARBA:ARBA00004906}.
CC -!- CAUTION: Lacks conserved residue(s) required for the propagation of
CC feature annotation. {ECO:0000256|PROSITE-ProRule:PRU00104}.
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DR AlphaFoldDB; G3U5A3; -.
DR Ensembl; ENSLAFT00000036108.1; ENSLAFP00000023011.1; ENSLAFG00000011976.3.
DR GeneTree; ENSGT00940000155466; -.
DR HOGENOM; CLU_002173_14_3_1; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000007646; Unassembled WGS sequence.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR CDD; cd08691; C2_NEDL1-like; 1.
DR CDD; cd00201; WW; 2.
DR Gene3D; 2.20.70.10; -; 2.
DR Gene3D; 2.60.40.2840; -; 1.
DR Gene3D; 2.60.40.150; C2 domain; 1.
DR Gene3D; 3.90.1750.10; Hect, E3 ligase catalytic domains; 1.
DR InterPro; IPR000008; C2_dom.
DR InterPro; IPR035892; C2_domain_sf.
DR InterPro; IPR037795; C2_HECW.
DR InterPro; IPR000569; HECT_dom.
DR InterPro; IPR035983; Hect_E3_ubiquitin_ligase.
DR InterPro; IPR040524; HECW1_helix.
DR InterPro; IPR032348; HECW_N.
DR InterPro; IPR001202; WW_dom.
DR InterPro; IPR036020; WW_dom_sf.
DR PANTHER; PTHR11254:SF127; E3 UBIQUITIN-PROTEIN LIGASE HECW2; 1.
DR PANTHER; PTHR11254; HECT DOMAIN UBIQUITIN-PROTEIN LIGASE; 1.
DR Pfam; PF00168; C2; 1.
DR Pfam; PF00632; HECT; 1.
DR Pfam; PF18436; HECW1_helix; 1.
DR Pfam; PF16562; HECW_N; 1.
DR Pfam; PF00397; WW; 2.
DR SMART; SM00239; C2; 1.
DR SMART; SM00456; WW; 2.
DR SUPFAM; SSF49562; C2 domain (Calcium/lipid-binding domain, CaLB); 1.
DR SUPFAM; SSF56204; Hect, E3 ligase catalytic domain; 1.
DR SUPFAM; SSF51045; WW domain; 2.
DR PROSITE; PS50004; C2; 1.
DR PROSITE; PS50237; HECT; 1.
DR PROSITE; PS01159; WW_DOMAIN_1; 2.
DR PROSITE; PS50020; WW_DOMAIN_2; 2.
PE 4: Predicted;
KW Reference proteome {ECO:0000313|Proteomes:UP000007646};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786,
KW ECO:0000256|PROSITE-ProRule:PRU00104}.
FT DOMAIN 65..196
FT /note="C2"
FT /evidence="ECO:0000259|PROSITE:PS50004"
FT DOMAIN 699..732
FT /note="WW"
FT /evidence="ECO:0000259|PROSITE:PS50020"
FT DOMAIN 877..910
FT /note="WW"
FT /evidence="ECO:0000259|PROSITE:PS50020"
FT DOMAIN 1132..1243
FT /note="HECT"
FT /evidence="ECO:0000259|PROSITE:PS50237"
FT REGION 237..346
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 388..688
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 916..961
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1056..1083
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 260..308
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 439..523
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 537..568
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 589..605
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 667..688
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1058..1076
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1243 AA; 137871 MW; B5B2A0E3767F52BD CRC64;
DSKNRGVTGT QKGQIVWRIE PGPYFMEPEI KICFKYYHGI SGALRATTPC ITVKNPAVMM
GAEGMEGGVS GSSHSRKLVS FTLSEDLRAV GLKKGMFFNP DPYLKMSIQP GKKSSFPTCA
HHGQERRSTI ISNTTNPIWH REKYSFFALL TDVLEIEIKD KFAKSRPIIK RFLGKLTIPV
QRLLERQAIG DQMLSYNLGR RLPADHVSGY LQFRVEVTSS VHEDASPEAV GTILGVSTVN
GDLGSPSDDE DMPGGHHDSQ VCSNGPTSEE SAADGTPKHS LRTSSTLEID TEELISTSSR
TSPPRGRQDS LNDYLDAIEH NGHSRPGAAT CSERSMGASP KLRSSFPTDT RLNAMLHIDS
DEEDHEFQQD LGYPSSLEEE GGLIMFSRAS RADDDGSLAS QTKPEDNPVE NEEAFANEAT
SFEDKPENHA ELADSTLPSG PSPDENENGP ESQQSADQGS GELCGSQEMD QPTSGADTGT
SDTSGGSRRA VSETESLDQG SEPSQVSSET EPSDPARTES VSEASTRPEG ESDLEGADSS
CNESVTTQLS SVETRCSSLE SARFPETPAF SSQEEEDGVC AAEPTSSGPA EGPQESVSTP
GSLPVVQVPS GEEEGPESAT LPDQEEMGAV WQRRGSLEGA TAENQPQEEG DAGDAQGTCE
GATAQDEGAT GGSQANGHQP LRSLPSVRQD VSRYQRVDEA LPPNWEARID SHGRIFYVDH
VNRTTTWQRP TAPPAPQMLQ RSNSIQQMEQ LNRRYQSIRR TMTNERPEEN TSALDGAGEE
ADFHQASADF RRENVLPHST SRSRLTLLLQ SPPVKFLISP EFFTVLHSNP SAYRMFTNNT
CLKHMITKVR RDTHHFERYQ HNRDLVGFLN MFANKQLELP RGWEMKHDHQ GKAFFVDHNS
RTTTFIDPRL PLQSSRPTSA LVHRQHLTRQ RSHSAGEVGE DSRHAGPPVL PRPSSTFNTV
SRPQYQDMVP VAYNDKIVAF LRQPNIFEIL QERQPDLTRN HSLSLNREKI QFIRTEGTPG
LVRLSSDADL VMLLSLFEEE IMSYVPPHAL LHPSYCQSPR GSPVSSPQNS PGTQRANARA
PAPYKRDFEA KLRNFYRKLE TKGYGQGPGK LKLIIRRDHL LEDAFNQIMG YSRKDLQRNK
LYVTFVGEEG LDYSGPSREF FFLVSRELFN PYYGLFEYSA NDTYTVQISP MSAFVDNHHE
WFRFSGRILG LALIHQYLLD AFFTRPFYKA LLRIVIICSL SQI
//
