ID G3VQJ8_SARHA Unreviewed; 998 AA.
AC G3VQJ8;
DT 16-NOV-2011, integrated into UniProtKB/TrEMBL.
DT 07-APR-2021, sequence version 2.
DT 27-MAR-2024, entry version 58.
DE RecName: Full=isoleucine--tRNA ligase {ECO:0000256|ARBA:ARBA00013165};
DE EC=6.1.1.5 {ECO:0000256|ARBA:ARBA00013165};
DE AltName: Full=Isoleucyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00032665};
GN Name=IARS2 {ECO:0000313|Ensembl:ENSSHAP00000005453.2};
OS Sarcophilus harrisii (Tasmanian devil) (Sarcophilus laniarius).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Metatheria; Dasyuromorphia; Dasyuridae; Sarcophilus.
OX NCBI_TaxID=9305 {ECO:0000313|Ensembl:ENSSHAP00000005453.2, ECO:0000313|Proteomes:UP000007648};
RN [1] {ECO:0000313|Ensembl:ENSSHAP00000005453.2, ECO:0000313|Proteomes:UP000007648}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=21709235; DOI=10.1073/pnas.1102838108;
RA Miller W., Hayes V.M., Ratan A., Petersen D.C., Wittekindt N.E., Miller J.,
RA Walenz B., Knight J., Qi J., Zhao F., Wang Q., Bedoya-Reina O.C.,
RA Katiyar N., Tomsho L.P., Kasson L.M., Hardie R.A., Woodbridge P.,
RA Tindall E.A., Bertelsen M.F., Dixon D., Pyecroft S., Helgen K.M.,
RA Lesk A.M., Pringle T.H., Patterson N., Zhang Y., Kreiss A., Woods G.M.,
RA Jones M.E., Schuster S.C.;
RT "Genetic diversity and population structure of the endangered marsupial
RT Sarcophilus harrisii (Tasmanian devil).";
RL Proc. Natl. Acad. Sci. U.S.A. 108:12348-12353(2011).
RN [2] {ECO:0000313|Ensembl:ENSSHAP00000005453.2}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000256|ARBA:ARBA00005594}.
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DR RefSeq; XP_003767767.1; XM_003767719.2.
DR AlphaFoldDB; G3VQJ8; -.
DR Ensembl; ENSSHAT00000005507.2; ENSSHAP00000005453.2; ENSSHAG00000004762.2.
DR GeneID; 100916992; -.
DR KEGG; shr:100916992; -.
DR CTD; 55699; -.
DR GeneTree; ENSGT00550000074910; -.
DR HOGENOM; CLU_001493_7_2_1; -.
DR InParanoid; G3VQJ8; -.
DR Proteomes; UP000007648; Unassembled WGS sequence.
DR GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004822; F:isoleucine-tRNA ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR GO; GO:0006428; P:isoleucyl-tRNA aminoacylation; IEA:InterPro.
DR CDD; cd07960; Anticodon_Ia_Ile_BEm; 1.
DR Gene3D; 1.10.730.20; -; 1.
DR Gene3D; 3.40.50.620; HUPs; 2.
DR Gene3D; 1.10.10.830; Ile-tRNA synthetase CP2 domain-like; 1.
DR HAMAP; MF_02002; Ile_tRNA_synth_type1; 1.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR033708; Anticodon_Ile_BEm.
DR InterPro; IPR002301; Ile-tRNA-ligase.
DR InterPro; IPR023585; Ile-tRNA-ligase_type1.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR NCBIfam; TIGR00392; ileS; 1.
DR PANTHER; PTHR42765:SF1; ISOLEUCINE--TRNA LIGASE, MITOCHONDRIAL; 1.
DR PANTHER; PTHR42765; SOLEUCYL-TRNA SYNTHETASE; 1.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF00133; tRNA-synt_1; 1.
DR PRINTS; PR00984; TRNASYNTHILE.
DR SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
DR SUPFAM; SSF50677; ValRS/IleRS/LeuRS editing domain; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Ligase {ECO:0000256|ARBA:ARBA00022598};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917};
KW Reference proteome {ECO:0000313|Proteomes:UP000007648}.
FT DOMAIN 89..701
FT /note="Aminoacyl-tRNA synthetase class Ia"
FT /evidence="ECO:0000259|Pfam:PF00133"
FT DOMAIN 746..870
FT /note="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase
FT anticodon-binding"
FT /evidence="ECO:0000259|Pfam:PF08264"
SQ SEQUENCE 998 AA; 112841 MW; 45B9881C73D94888 CRC64;
MLWRLSTGSL GALRRPQWGP GWRGTKTTSF LRRAKSVNGA HDQQTPAKAS PHRQTVLLPK
TSMPMKLVGR QQTDMELEIQ QKCGFSKLYS WQEKRNVREN FCLHDGPPYA NGEAHVGHAL
NKILKDITNR FQMMRGAKVH FVPGWDCHGL PIELKALSEL GEKAQNLSPM EIRKTARAFA
EAAIEKQKSA FIRWGIMADW DNCYYTFDKK YEAQQLKMFY QMYTKDLIYR AYKPVFWSPS
ARTALAEAEL EYNPKHVSHS IYAKFPLLKP PPLLASVLEG SSPVSAIIWT TQPWTIPANQ
AVCYMPESNY SIVKCTNSGE FYILAAEKVS SVATVLGTEF EVISTFLGAD LENGSCVHPI
IADRVSPLLP AQHVNLSKGT GMVHIAPAHG MEDYIVASRH ELPMDCLVDE DGNFTEAAGS
ELENKNALEE GTDTVIKMLQ STKNLLKEED ITHSYPYDWR TKKPVIIRSS KQWFVKIGNM
TATAKESLKK VKFIPASTMN SMVDMTERRP YWCISRQRVW GVPIPVFHHK TKDEVLINSE
TIDHLVQLVE KHGSDIWWTL PPEKLLPQKV LSQLGDPDAL EYVPGQDILD IWFESGTSWN
CVLPDQRADL YLEGKDQIGG WFQSSLLTSV AARKKAPFRV LLVHGFTLSD KGEKMSKSLG
NVISPDVIIN GGQDHSKDPP YGADILRWWV AESHIFSEVK IGSRILNAAR DNIDKLRNTL
RFLLGNLADF NPKTDSVPFE EMYIIDQYML HLLQDFGKKV TDAYRQYDFG KVIRHLQAFY
PSQLSNFYFS IIKDRLYCEK ENDLKRRSCQ TALAEILDVM VRAIAPILPH LAEEVFQHIP
YEKEPKSVFR SGWITTSTLW KKPGLEEAVE AACGMRDAFL GSIPGKNSLE YDVTVVIDPG
LLFEIIEMLH EQKTSGMSQL SELMMASQLS LLAQDPIAIP ADAIELKGSY EINLEGICEQ
SSYKVIVTPS TKEKCPRCWK YASDSPNELC PRCTEVLA
//
