ID G3X795_BOVIN Unreviewed; 704 AA.
AC G3X795;
DT 16-NOV-2011, integrated into UniProtKB/TrEMBL.
DT 10-APR-2019, sequence version 2.
DT 27-MAR-2024, entry version 72.
DE RecName: Full=Neurolysin, mitochondrial {ECO:0000256|ARBA:ARBA00039454};
DE EC=3.4.24.16 {ECO:0000256|ARBA:ARBA00039068};
DE AltName: Full=Microsomal endopeptidase {ECO:0000256|ARBA:ARBA00041940};
DE AltName: Full=Mitochondrial oligopeptidase M {ECO:0000256|ARBA:ARBA00042607};
DE AltName: Full=Neurotensin endopeptidase {ECO:0000256|ARBA:ARBA00041659};
GN Name=NLN {ECO:0000313|Ensembl:ENSBTAP00000022482.6,
GN ECO:0000313|VGNC:VGNC:97293};
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913 {ECO:0000313|Ensembl:ENSBTAP00000022482.6, ECO:0000313|Proteomes:UP000009136};
RN [1] {ECO:0000313|Ensembl:ENSBTAP00000022482.6, ECO:0000313|Proteomes:UP000009136}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Hereford {ECO:0000313|Ensembl:ENSBTAP00000022482.6,
RC ECO:0000313|Proteomes:UP000009136};
RA Rosen B.D., Bickhart D.M., Koren S., Schnabel R.D., Hall R., Zimin A.,
RA Dreischer C., Schultheiss S., Schroeder S.G., Elsik C.G., Couldrey C.,
RA Liu G.E., Van Tassell C.P., Phillippy A.M., Smith T.P.L., Medrano J.F.;
RT "ARS-UCD1.2.";
RL Submitted (MAR-2018) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSBTAP00000022482.6}
RP IDENTIFICATION.
RC STRAIN=Hereford {ECO:0000313|Ensembl:ENSBTAP00000022482.6};
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Preferential cleavage in neurotensin: 10-Pro-|-Tyr-11.;
CC EC=3.4.24.16; Evidence={ECO:0000256|ARBA:ARBA00035987};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|RuleBase:RU003435};
CC Note=Binds 1 zinc ion. {ECO:0000256|RuleBase:RU003435};
CC -!- SIMILARITY: Belongs to the peptidase M3 family.
CC {ECO:0000256|ARBA:ARBA00006040, ECO:0000256|RuleBase:RU003435}.
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DR AlphaFoldDB; G3X795; -.
DR SMR; G3X795; -.
DR Ensembl; ENSBTAT00000022482.6; ENSBTAP00000022482.6; ENSBTAG00000016903.6.
DR VEuPathDB; HostDB:ENSBTAG00000016903; -.
DR VGNC; VGNC:97293; NLN.
DR GeneTree; ENSGT00950000183171; -.
DR HOGENOM; CLU_001805_2_1_1; -.
DR InParanoid; G3X795; -.
DR OMA; RSGAWCS; -.
DR TreeFam; TF300459; -.
DR Reactome; R-BTA-375276; Peptide ligand-binding receptors.
DR Proteomes; UP000009136; Chromosome 20.
DR Bgee; ENSBTAG00000016903; Expressed in midbrain and 103 other cell types or tissues.
DR GO; GO:0005758; C:mitochondrial intermembrane space; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IEA:Ensembl.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004222; F:metalloendopeptidase activity; IBA:GO_Central.
DR GO; GO:0042277; F:peptide binding; IEA:Ensembl.
DR GO; GO:0006518; P:peptide metabolic process; IBA:GO_Central.
DR GO; GO:0006508; P:proteolysis; IBA:GO_Central.
DR GO; GO:0006111; P:regulation of gluconeogenesis; IEA:Ensembl.
DR GO; GO:1902809; P:regulation of skeletal muscle fiber differentiation; IEA:Ensembl.
DR CDD; cd06455; M3A_TOP; 1.
DR Gene3D; 3.40.390.10; Collagenase (Catalytic Domain); 1.
DR Gene3D; 1.10.1370.10; Neurolysin, domain 3; 1.
DR InterPro; IPR024079; MetalloPept_cat_dom_sf.
DR InterPro; IPR024077; Neurolysin/TOP_dom2.
DR InterPro; IPR024080; Neurolysin/TOP_N.
DR InterPro; IPR045090; Pept_M3A_M3B.
DR InterPro; IPR001567; Pept_M3A_M3B_dom.
DR PANTHER; PTHR11804:SF44; NEUROLYSIN, MITOCHONDRIAL; 1.
DR PANTHER; PTHR11804; PROTEASE M3 THIMET OLIGOPEPTIDASE-RELATED; 1.
DR Pfam; PF01432; Peptidase_M3; 1.
DR SUPFAM; SSF55486; Metalloproteases ('zincins'), catalytic domain; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU003435};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|RuleBase:RU003435};
KW Metalloprotease {ECO:0000256|ARBA:ARBA00023049,
KW ECO:0000256|RuleBase:RU003435};
KW Protease {ECO:0000256|ARBA:ARBA00022670, ECO:0000256|RuleBase:RU003435};
KW Reference proteome {ECO:0000313|Proteomes:UP000009136};
KW Zinc {ECO:0000256|ARBA:ARBA00022833, ECO:0000256|RuleBase:RU003435}.
FT DOMAIN 251..700
FT /note="Peptidase M3A/M3B catalytic"
FT /evidence="ECO:0000259|Pfam:PF01432"
SQ SEQUENCE 704 AA; 80745 MW; 77DC71F5E5C6DE3F CRC64;
MIVQCLLAVR GLHRVGGSRI LFRMTLGREE MSPLQAMSSY MAAGRNVLRW DLSPEQIKTR
TEELISQTKQ VYDAIGMRDI KEVTYENCLQ ALADIEVKYI VERTMLDFPQ HVSSDKEVRA
ASTEADKRLS RFDIEMSMRQ DIFLRIVHLK ETCDLEKIKP EARRYLEKSV KMGKRNGLHL
PEQVQNEIKA MKKRMSELCI DFNKNLNEDD TFLVFSKAEL GALPDDFINS LEKTDGDKYK
ITLKYPHYFP VMKKCCVPET RRKMEMAFNT RCKEENTVIL QQLLPLRAEV ARLLGYSTHA
DFVLEMNTAK STRHVTAFLD DLSQKLKLLG EAEREFILNL KKKECEERGF EYDGKINAWD
LHYYMTQTEE LKYSVDQETL KEYFPIEVVT EGLLNIYQEL LGLSFEQVTD AHVWNKSVTL
YTVKDKATGE VLGQFYLDLY PREGKYNHAA CFGLQPGCLL PDGSRMMSVA ALVVNFSQPL
AGRPSLLRHD EVRTYFHEFG HVMHQICAQT DFARFSGTNV ETDFVEVPSQ MLENWVWDAD
SLRRLSKHYR HGSPITDDLL EKLVASRLVN TGLLTLRQIV LSKVDQSLHT NTALDAASEY
AKYCTEILGV AATPGTNMPA TFGHLAGGYD GQYYGYLWSE VFSMDMFYSC FKKEGIMNPE
VGMKYRNLIL KPGGSLDGMD MLQNFLTREP NQKAFLMSRG LPAP
//
