ID G3XCX1_PSEAE Unreviewed; 1261 AA.
AC G3XCX1;
DT 16-NOV-2011, integrated into UniProtKB/TrEMBL.
DT 16-NOV-2011, sequence version 1.
DT 27-MAR-2024, entry version 74.
DE RecName: Full=nitrate reductase (quinone) {ECO:0000256|ARBA:ARBA00012500};
DE EC=1.7.5.1 {ECO:0000256|ARBA:ARBA00012500};
GN Name=narG {ECO:0000313|EMBL:AAG07262.1};
GN OrderedLocusNames=PA3875 {ECO:0000313|EMBL:AAG07262.1};
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964 {ECO:0000313|EMBL:AAG07262.1, ECO:0000313|Proteomes:UP000002438};
RN [1] {ECO:0000313|EMBL:AAG07262.1, ECO:0000313|Proteomes:UP000002438}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 /
RC 1C / PRS 101 / PAO1 {ECO:0000313|Proteomes:UP000002438};
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.Q., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R., Smith K.,
RA Spencer D., Wong G.K., Wu Z., Paulsen I.T., Reizer J., Saier M.H.,
RA Hancock R.E., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a quinol + nitrate = a quinone + H2O + nitrite;
CC Xref=Rhea:RHEA:56144, ChEBI:CHEBI:15377, ChEBI:CHEBI:16301,
CC ChEBI:CHEBI:17632, ChEBI:CHEBI:24646, ChEBI:CHEBI:132124; EC=1.7.5.1;
CC Evidence={ECO:0000256|ARBA:ARBA00001854};
CC -!- COFACTOR:
CC Name=Mo-bis(molybdopterin guanine dinucleotide);
CC Xref=ChEBI:CHEBI:60539; Evidence={ECO:0000256|ARBA:ARBA00001942};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004202};
CC Peripheral membrane protein {ECO:0000256|ARBA:ARBA00004202}. Membrane
CC {ECO:0000256|ARBA:ARBA00004170}; Peripheral membrane protein
CC {ECO:0000256|ARBA:ARBA00004170}.
CC -!- SIMILARITY: Belongs to the prokaryotic molybdopterin-containing
CC oxidoreductase family. {ECO:0000256|ARBA:ARBA00010312}.
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DR EMBL; AE004091; AAG07262.1; -; Genomic_DNA.
DR PIR; G83162; G83162.
DR RefSeq; NP_252564.1; NC_002516.2.
DR RefSeq; WP_003105302.1; NZ_QZGE01000001.1.
DR AlphaFoldDB; G3XCX1; -.
DR SMR; G3XCX1; -.
DR STRING; 208964.PA3875; -.
DR PaxDb; 208964-PA3875; -.
DR GeneID; 879780; -.
DR KEGG; pae:PA3875; -.
DR PATRIC; fig|208964.12.peg.4058; -.
DR PseudoCAP; PA3875; -.
DR HOGENOM; CLU_000422_14_1_6; -.
DR InParanoid; G3XCX1; -.
DR OrthoDB; 9759518at2; -.
DR PhylomeDB; G3XCX1; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0009325; C:nitrate reductase complex; IEA:InterPro.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0043546; F:molybdopterin cofactor binding; IEA:InterPro.
DR GO; GO:0008940; F:nitrate reductase activity; IEA:InterPro.
DR GO; GO:0009061; P:anaerobic respiration; IBA:GO_Central.
DR GO; GO:0042126; P:nitrate metabolic process; IEA:InterPro.
DR CDD; cd02776; MopB_CT_Nitrate-R-NarG-like; 1.
DR CDD; cd02750; MopB_Nitrate-R-NarG-like; 1.
DR Gene3D; 3.40.50.12440; -; 1.
DR Gene3D; 4.10.1200.10; nitrate reductase tail; 1.
DR InterPro; IPR009010; Asp_de-COase-like_dom_sf.
DR InterPro; IPR037943; MopB_CT_Nitrate-R-NarG-like.
DR InterPro; IPR006657; MoPterin_dinucl-bd_dom.
DR InterPro; IPR006656; Mopterin_OxRdtase.
DR InterPro; IPR006963; Mopterin_OxRdtase_4Fe-4S_dom.
DR InterPro; IPR006655; Mopterin_OxRdtase_prok_CS.
DR InterPro; IPR027467; MopterinOxRdtase_cofactor_BS.
DR InterPro; IPR006468; NarG.
DR InterPro; IPR028189; Nitr_red_alph_N.
DR InterPro; IPR044906; Nitr_red_alph_N_sf.
DR NCBIfam; TIGR01580; narG; 1.
DR PANTHER; PTHR43105; RESPIRATORY NITRATE REDUCTASE; 1.
DR PANTHER; PTHR43105:SF2; RESPIRATORY NITRATE REDUCTASE 2 ALPHA CHAIN; 1.
DR Pfam; PF00384; Molybdopterin; 1.
DR Pfam; PF01568; Molydop_binding; 1.
DR Pfam; PF14710; Nitr_red_alph_N; 1.
DR SMART; SM00926; Molybdop_Fe4S4; 1.
DR SUPFAM; SSF50692; ADC-like; 1.
DR SUPFAM; SSF53706; Formate dehydrogenase/DMSO reductase, domains 1-3; 1.
DR PROSITE; PS51669; 4FE4S_MOW_BIS_MGD; 1.
DR PROSITE; PS00551; MOLYBDOPTERIN_PROK_1; 1.
DR PROSITE; PS00490; MOLYBDOPTERIN_PROK_2; 1.
DR PROSITE; PS00932; MOLYBDOPTERIN_PROK_3; 1.
PE 3: Inferred from homology;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Membrane {ECO:0000256|ARBA:ARBA00022475};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000002438}.
FT DOMAIN 43..107
FT /note="4Fe-4S Mo/W bis-MGD-type"
FT /evidence="ECO:0000259|PROSITE:PS51669"
SQ SEQUENCE 1261 AA; 140973 MW; 30CA8AF6827EB3C6 CRC64;
MSHLLDRLQF FKKKQGEFAD GHGETSNESR AWEGAYRQRW QHDKIVRSTH GVNCTGSCSW
KIYVKNGLIT WETQQTDYPR TRPDLPNHEP RGCPRGASYS WYIYSANRLK YPKVRKPLLK
LWREARAQHG DPVNAWASIV EDAAKAKSYK SQRGLGGFVR SSWDEVTEII AAANVYTAKT
YGPDRVIGFS PIPAMSMVSY AAGARYLSLI GGVCLSFYDW YCDLPPASPQ IWGEQTDVPE
SADWYNSSYI IAWGSNVPQT RTPDAHFFTE VRYKGTKTVS ITPDYSEVAK LTDLWLNPKQ
GTDAALGMAF GHVILKEFHL DRPSAYFVDY CRQYTDMPML VLLEEHAGGA FKPTRYLRAA
DLADNLGQDN NPEWKTIAYD ERSGGLVSPT GAIGYRWGES GKWNIAELDG RSGDQTRLQL
SLLDGPEHAC EVAFPYFAGQ EHPHFKGVAN DEVLLRRVPF REIVAADGKR LRVATVYDLQ
MANYSIDRGL GGDNVATSYE DADTPYTPAW QERITGVPAA RATQVAREFA DSADKTRGKA
MVIIGAAMNH WYHMDMNYRA VINMLMMCGC IGQSGGGWAH YVGQEKLRPQ TGWAPLAFGL
DWSRPPRQMN GTSFFYLHSS QWRHEKLSMH EVLSPLADAS RFAEHALDYN IQAERLGWLP
SAPQLNRNPL RIAAEAEAAG LPVADYVVRE LKSGGLRFAS ESPDDPQNFP RNMFIWRSNL
LGSSGKGHEY MLKYLLGAKN GVMNDDLGKA GGPRPTEVDW VDDGAEGKLD LVTTLDFRMS
STCMYSDIVL PTATWYEKDD LNTSDMHPFI HPLSAATDPA WEAKSDWEIY KAIAKKFSAV
AEGHLGVEQD LVTVPLLHDT PTELAQPFGG DGHDWKKGEC EPVPGRNLPT LHLVERDYPN
VYRKFTSLGP LLDKLGNGGK GIGWNTEKEV KLVGDLNHRV VESGVSQGRP RIDSAIDAAE
VVLALAPETN GQVAVKAWEA LSKITGREHA HLALPKEDEK IRFRDIQVQP RKIISSPTWS
GLEDEHVSYN AGYTNVHELI PWRTITGRQQ FYQDHPWMQA FGEGFVSYRP PVNTRTTEKL
LNRKPNGNPE ITLNWITPHQ KWGIHSTYSD NLLMLTLSRG GPIIWLSEHD AAKAGIVDND
WVEVFNANGA ATCRAVVSQR VKDGMVMMYH AQERIVNVPG SETTGTRGGH HNSVTRVVLK
PTHMIGGYAQ QAWGFNYYGT VGCNRDEFVV VRKMSKVDWL DEPRHGGLGG DALPQPLPQD
I
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