ID G3XN54_ASPNA Unreviewed; 2461 AA.
AC G3XN54;
DT 14-DEC-2011, integrated into UniProtKB/TrEMBL.
DT 14-DEC-2011, sequence version 1.
DT 27-MAR-2024, entry version 60.
DE RecName: Full=non-specific serine/threonine protein kinase {ECO:0000256|ARBA:ARBA00012513};
DE EC=2.7.11.1 {ECO:0000256|ARBA:ARBA00012513};
GN ORFNames=ASPNIDRAFT_210817 {ECO:0000313|EMBL:EHA27521.1};
OS Aspergillus niger (strain ATCC 1015 / CBS 113.46 / FGSC A1144 / LSHB Ac4 /
OS NCTC 3858a / NRRL 328 / USDA 3528.7).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC Aspergillus subgen. Circumdati.
OX NCBI_TaxID=380704 {ECO:0000313|EMBL:EHA27521.1, ECO:0000313|Proteomes:UP000009038};
RN [1] {ECO:0000313|EMBL:EHA27521.1, ECO:0000313|Proteomes:UP000009038}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 1015 / CBS 113.46 / FGSC A1144 / LSHB Ac4 / NCTC 3858a /
RC NRRL 328 / USDA 3528.7 {ECO:0000313|Proteomes:UP000009038};
RX PubMed=21543515; DOI=10.1101/gr.112169.110;
RA Andersen M.R., Salazar M.P., Schaap P.J., van de Vondervoort P.J.,
RA Culley D., Thykaer J., Frisvad J.C., Nielsen K.F., Albang R., Albermann K.,
RA Berka R.M., Braus G.H., Braus-Stromeyer S.A., Corrochano L.M., Dai Z.,
RA van Dijck P.W., Hofmann G., Lasure L.L., Magnuson J.K., Menke H.,
RA Meijer M., Meijer S.L., Nielsen J.B., Nielsen M.L., van Ooyen A.J.,
RA Pel H.J., Poulsen L., Samson R.A., Stam H., Tsang A., van den Brink J.M.,
RA Atkins A., Aerts A., Shapiro H., Pangilinan J., Salamov A., Lou Y.,
RA Lindquist E., Lucas S., Grimwood J., Grigoriev I.V., Kubicek C.P.,
RA Martinez D., van Peij N.N., Roubos J.A., Nielsen J., Baker S.E.;
RT "Comparative genomics of citric-acid-producing Aspergillus niger ATCC 1015
RT versus enzyme-producing CBS 513.88.";
RL Genome Res. 21:885-897(2011).
CC -!- FUNCTION: Serine/threonine protein kinase which activates checkpoint
CC signaling upon genotoxic stresses such as ionizing radiation (IR),
CC ultraviolet light (UV), or DNA replication stalling, thereby acting as
CC a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-
CC Q. Phosphorylates histone H2A to form H2AS128ph (gamma-H2A) at sites of
CC DNA damage, involved in the regulation of DNA damage response
CC mechanism. Required for the control of telomere length and genome
CC stability. {ECO:0000256|ARBA:ARBA00025079}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
CC [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1;
CC Evidence={ECO:0000256|ARBA:ARBA00001433};
CC -!- SUBUNIT: Associates with DNA double-strand breaks.
CC {ECO:0000256|ARBA:ARBA00011370}.
CC -!- SIMILARITY: Belongs to the PI3/PI4-kinase family. ATM subfamily.
CC {ECO:0000256|ARBA:ARBA00010769}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EHA27521.1}.
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DR EMBL; ACJE01000002; EHA27521.1; -; Genomic_DNA.
DR STRING; 380704.G3XN54; -.
DR HOGENOM; CLU_000178_4_1_1; -.
DR Proteomes; UP000009038; Unassembled WGS sequence.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-KW.
DR GO; GO:0032991; C:protein-containing complex; IEA:UniProt.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0006325; P:chromatin organization; IEA:UniProtKB-KW.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR GO; GO:0035556; P:intracellular signal transduction; IEA:UniProt.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR GO; GO:0019219; P:regulation of nucleobase-containing compound metabolic process; IEA:UniProt.
DR CDD; cd00892; PIKKc_ATR; 1.
DR Gene3D; 1.10.1070.11; Phosphatidylinositol 3-/4-kinase, catalytic domain; 1.
DR Gene3D; 1.25.40.10; Tetratricopeptide repeat domain; 1.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR003152; FATC_dom.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR000403; PI3/4_kinase_cat_dom.
DR InterPro; IPR036940; PI3/4_kinase_cat_sf.
DR InterPro; IPR018936; PI3/4_kinase_CS.
DR InterPro; IPR003151; PIK-rel_kinase_FAT.
DR InterPro; IPR014009; PIK_FAT.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR InterPro; IPR012993; UME.
DR PANTHER; PTHR11139; ATAXIA TELANGIECTASIA MUTATED ATM -RELATED; 1.
DR PANTHER; PTHR11139:SF124; SERINE_THREONINE-PROTEIN KINASE MEC1; 1.
DR Pfam; PF02259; FAT; 1.
DR Pfam; PF02260; FATC; 1.
DR Pfam; PF00454; PI3_PI4_kinase; 1.
DR Pfam; PF08064; UME; 1.
DR SMART; SM01343; FATC; 1.
DR SMART; SM00146; PI3Kc; 1.
DR SMART; SM00802; UME; 1.
DR SUPFAM; SSF48371; ARM repeat; 1.
DR SUPFAM; SSF56112; Protein kinase-like (PK-like); 1.
DR SUPFAM; SSF48452; TPR-like; 1.
DR PROSITE; PS51189; FAT; 1.
DR PROSITE; PS51190; FATC; 1.
DR PROSITE; PS00916; PI3_4_KINASE_2; 1.
DR PROSITE; PS50290; PI3_4_KINASE_3; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Chromatin regulator {ECO:0000256|ARBA:ARBA00022853};
KW DNA damage {ECO:0000256|ARBA:ARBA00023204};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW Kinase {ECO:0000256|ARBA:ARBA00022777};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Serine/threonine-protein kinase {ECO:0000256|ARBA:ARBA00022527};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 1436..2012
FT /note="FAT"
FT /evidence="ECO:0000259|PROSITE:PS51189"
FT DOMAIN 2125..2445
FT /note="PI3K/PI4K catalytic"
FT /evidence="ECO:0000259|PROSITE:PS50290"
FT DOMAIN 2429..2461
FT /note="FATC"
FT /evidence="ECO:0000259|PROSITE:PS51190"
SQ SEQUENCE 2461 AA; 277855 MW; CC77AD54BABD4FC1 CRC64;
MASNKRKESR LPIPGIADWN PAEPVSSTLA ANLVPHLSSQ GDGTPGLTKE AFSQLRHELL
EGKYSHLQLN DSITDVKKLI CIVLKAGLEP CIRDDRSELQ GQLLDCLDIV QNAVDKAPLA
LLEMPEPELL GKDAAAPLYS CLVVWLVHLF STWDSEPVED RIGGILASIF NAPHNNTRLW
SSFVGITALI QACTTELLSA LEAYQSRARL THVASSSVVN PKQLCTLDLD RLGLRCASFQ
KLIRLESCAQ AVRLCLNLLI SAINAYVSYN IPSKRQNLAW LSNGYRRLWK LTVLWHCKSD
VKDVTSQIPT FSRFLRFIKR FCDHVPYTSP KKRLACIFLH IFAETLALEI LNQASDLQLS
LSQAIDSFTN AFTSEKDIMR SMRATILPVK SKLALFNSFD TCLQAAIESS LVHCPIVYNS
LDAKDAEAAF CSGNIPEMAA VHGFPGSGHS SSSRVGAVQM AKRRCLPRLD ADRESRNVAQ
RLIAKSLKLL RCDQSGTLGD LSCAVATSYS GLSEQDKLSL LCVLCTIPCA LSGNLTDIKN
VDSEDSFTCR ICDPKCRDLK SESNSQTVKS DELCQILDHI ISNLSRTANL RIAATVALRR
TLMHTSESAH MQLTSSVFGE FCLNFLRSSV RELRLTTGHS ITSFVRRNLD HETRRSNFIV
ILDWLQNLSQ KQETPLHETC ILTLCHLAQF SNDEEMNIIL LRLVEYLGHS NPFIGAVAYT
ELAGLAQHLS VTPAALFRPF WRTLSVTVVR NFQSRPYMAE QLCDLLGMKV DDFLRLTETY
VLPYLVLARK RDVIIRVGAT YKDAKTPFDI CSEKNNLASI LAFLLSQPSP NLEESVMSAL
SEVDSAFRGR TLAELLRIEP ILIACDLLKG LGDLGEDKGK RFLRALHHLA ILVPRKSGPT
PRRANLIGYF VEEHVLGIIT QFAHAVNDFQ IRQPLIEKKR NIAAIGEMIK VARGHISSAL
PQICACLRAA LEIQELCNNA FIVWRVLISS LQEEEVEPLI DQTLSIVIRY WDMFHEDTRK
QACELVEYIM QSHYELVRDI FNTMPSLASI PILYKHEATL NSMKETMDVR SHFLAFIRRC
QSDTATVVEQ SLRELVPYLS QNEEFIHRSV LSEIPDPVVA QLARSLLDCC VKFNTSSDVI
TKLSAECLGL IGCLDPNRVD TVKEKKGILV LSNFDSMEET FDFILFFLQN VLVETFLSAS
NTRAQGFLAY AMQNLLRFCG LDSAVANRSR DFRADEKYQR WLELPETVRN ILAPFLTSKY
TVTIGTLNTN CSYPLFTAGL THGNWLRSFV QDLLQAGNGD NAKLVFSVCS RIVKGQDISI
ASFLLPFAVL NRTVSGSAKE KEDLRCELVN VLSHPLPDTH DYVYENIILC SQSIFEVLDY
LSRWLQGKKK QINGLRNHSY PSNRAHKGAS RDALLDTYYS QAKAVEILLS SIPPEVISKR
AVECKSFSRA LFHWEQYIRQ WKNQHSKQGG AVVEPLYQRL QDIYSRIDEP DGIEGISSHL
HVLNIDQQVL EHRKAGRWAT AQSWYELQLE KEPNNSEAQW NLLACLKESG QQDAILTRFE
ILKANSSSRK FLPFAVEATW ITSNWGKLNE YLQQLSQLGR GEFNIEIGLA LNAFRHGKYT
EFWEHMEALR LSVAKSLTAN SVVSLQSCHD SILKLHTLHE VESIARAKIK DCGSSDSRSK
LPDILDRRLD ILGGYISDKQ YLLGLRRATM ELTNEFTDSD IAAAWLTSAR LSRKSNFTNQ
AYQSMLHAAR LNDRSATIEH ARLLWKDGHH RKAIQTLEGA IAANEFSSET TSSQERTSSL
ITNNGEHQNL LLARAHLLLA KWTDRAGQTQ SDAIVQRYRE AIKLHPRWEK AHYYLGKHYN
KILDLEKAKP LGREAQIYLS GEASKLVVDN YLRSLAHGNK YVFQSLPKIL TLWLEHASTV
DQPFDPKRGD NEDFQKHTLN QRKKSLDDMH TQLRKYVNRI PAALLFTILP QVVARICHPN
TTVYDLLTKI VARAVNSFPQ QGLWTVLAVV KSSSKDRASR GISCLQKITE VNKKLKNQPL
SDMRGMINQG QKFSEEMLRL CIARIEDKVA RVSLRDLKFS HKVAPCRLVV PFQAMLTPTL
PASHKPEYLK GFRAFPRDPT TIEAIMDDVQ VLNSLQKPRK IGIRGSDGKM YNILCKPKDD
LRKDQRLMEF NNMINRLFKK DVESSKRRMY IKTYAVTPLN EECGLIEWVD NLRALRDIVI
KLLRERGIAP NYNEIRHDLN EACSDNSKLH LFTTRILAKF PPVLYEWFIE MFPEAGSWFA
ARIRYTRSCA VMSMVGHVLG LGDRHGENIL FEEGTGGVLH VDFNCLFDKG LTFDIPELVP
FRLTQNMVDA FGVYGYNGPF RKTCEISLGL LRHNEDALMT VLETFLHDPT TDFIGKKKRR
THVNAPETPA GVLENVRNKL RGLLPGESVP LSVDGHVDEL IVQATDVRNL AAMYIGWCAF
F
//
