ID G4ST95_META2 Unreviewed; 824 AA.
AC G4ST95;
DT 14-DEC-2011, integrated into UniProtKB/TrEMBL.
DT 14-DEC-2011, sequence version 1.
DT 27-MAR-2024, entry version 59.
DE SubName: Full=CRISPR-associated helicase Cas3 domain protein {ECO:0000313|EMBL:CCE23848.1};
GN Name=cas {ECO:0000313|EMBL:CCE23848.1};
GN OrderedLocusNames=MEALZ_2162 {ECO:0000313|EMBL:CCE23848.1};
OS Methylotuvimicrobium alcaliphilum (strain DSM 19304 / NCIMB 14124 / VKM
OS B-2133 / 20Z) (Methylomicrobium alcaliphilum).
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Methylococcales;
OC Methylococcaceae; Methylotuvimicrobium.
OX NCBI_TaxID=1091494 {ECO:0000313|EMBL:CCE23848.1, ECO:0000313|Proteomes:UP000008315};
RN [1] {ECO:0000313|Proteomes:UP000008315}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 19304 / NCIMB 14124 / VKM B-2133 / 20Z
RC {ECO:0000313|Proteomes:UP000008315};
RX PubMed=22207753; DOI=10.1128/JB.06392-11;
RA Vuilleumier S., Khmelenina V.N., Bringel F., Reshetnikov A.S., Lajus A.,
RA Mangenot S., Rouy Z., Op den Camp H.J., Jetten M.S., Dispirito A.A.,
RA Dunfield P., Klotz M.G., Semrau J.D., Stein L.Y., Barbe V., Medigue C.,
RA Trotsenko Y.A., Kalyuzhnaya M.G.;
RT "Genome sequence of the haloalkaliphilic methanotrophic bacterium
RT Methylomicrobium alcaliphilum 20Z.";
RL J. Bacteriol. 194:551-552(2012).
CC -!- SIMILARITY: In the N-terminal section; belongs to the CRISPR-associated
CC nuclease Cas3-HD family. {ECO:0000256|ARBA:ARBA00006847}.
CC -!- SIMILARITY: In the central section; belongs to the CRISPR-associated
CC helicase Cas3 family. {ECO:0000256|ARBA:ARBA00009046}.
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DR EMBL; FO082060; CCE23848.1; -; Genomic_DNA.
DR AlphaFoldDB; G4ST95; -.
DR STRING; 1091494.MEALZ_2162; -.
DR KEGG; mah:MEALZ_2162; -.
DR PATRIC; fig|271065.3.peg.2223; -.
DR HOGENOM; CLU_010123_0_0_6; -.
DR Proteomes; UP000008315; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR CDD; cd09641; Cas3''_I; 1.
DR CDD; cd17930; DEXHc_cas3; 1.
DR Gene3D; 1.10.3210.30; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR006483; CRISPR-assoc_Cas3_HD.
DR InterPro; IPR038257; CRISPR-assoc_Cas3_HD_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR006474; Helicase_Cas3_CRISPR-ass_core.
DR InterPro; IPR027417; P-loop_NTPase.
DR NCBIfam; TIGR01587; cas3_core; 1.
DR NCBIfam; TIGR01596; cas3_HD; 1.
DR Pfam; PF18019; Cas3_HD; 1.
DR Pfam; PF00270; DEAD; 1.
DR SUPFAM; SSF109604; HD-domain/PDEase-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51643; HD_CAS3; 1.
PE 3: Inferred from homology;
KW Antiviral defense {ECO:0000256|ARBA:ARBA00023118};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Reference proteome {ECO:0000313|Proteomes:UP000008315}.
FT DOMAIN 21..230
FT /note="HD Cas3-type"
FT /evidence="ECO:0000259|PROSITE:PS51643"
SQ SEQUENCE 824 AA; 92431 MW; C25278767BE91C55 CRC64;
MYRDRILKIK DTLYVAHVRS NDKKIQTLEE HLIDVAEIAK KLATKINLPE AGELIGLLHD
FGKYSTEFQN YLQSATGLID PDIDNEAVDA KALKGKIDHS SAGAQWIWQE LSRYGNNGEG
RLCGQIMAVC IASHHSGLID CLKPDGSNGF NVRMQKDDVR THLAECKENA DSQILERAKK
LMDKPLLKAM LEQIRVMMMS GSKQSTTIRE FYLGFWTRFL FSCLIDADRI DSADFETPGN
ACYRNKNDLQ LTVAIERLEL FLAGFSNKPT ESNTPIDGIR REISEVCKNR AHEPQGIYTL
TVPTGGGKTY ASLRYALHHA QKHKLERIIY VIPYTSIIEQ NAAAIRSALE RESDAYPWVL
EQHSNLEPER QTWHSKLAAE NWDAPIVLTT MVQFLETLFG GGTRGVRRLH QLANSVVIFD
EIQTLPIQCT HLFCNALNFL ADHAGMTAVL CTATQPVLDR LRASDKGQLS LPQNSELMPD
ISGLFDQLKR VVISDKTKPE GWSEDEIAEL ALTELDTKGN CLVIVNTKAW AQALYLKCAE
TVDIESLFHL STGQCAAHRK ALFKLMRQRL ADGLPVLCIS TQLIEAGVDI DFAGVVRFLA
GLDSIAQAAG RCNRNGHLST ATVHIVNPAR ETISQLIDIK VGQEKTKRVL SEIEDEDLLS
PEAMRRYFQY YFYDRADDMS YPLSAKQVGR DDSLLNLLSQ NKYNVGVGAA LHLKQSFMTA
GNVFKAIDAP THSVIVPYLE GKQLLVELGG IAKRFEARRF RECLQRAQQY SVNVFPNVWR
RLVEQQAVYE IQPGEGIYGL DERYYSEAFG LSTEPVSKAE AIIL
//
