ID G4XGW2_VITVI Unreviewed; 389 AA.
AC G4XGW2;
DT 14-DEC-2011, integrated into UniProtKB/TrEMBL.
DT 14-DEC-2011, sequence version 1.
DT 27-MAR-2024, entry version 43.
DE RecName: Full=trihydroxystilbene synthase {ECO:0000256|ARBA:ARBA00044056};
DE EC=2.3.1.95 {ECO:0000256|ARBA:ARBA00044056};
OS Vitis vinifera (Grape).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; Vitales; Vitaceae; Viteae; Vitis.
OX NCBI_TaxID=29760 {ECO:0000313|EMBL:AEP17003.1};
RN [1] {ECO:0000313|EMBL:AEP17003.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Fruit {ECO:0000313|EMBL:AEP17003.1};
RA Fang J.G., Zhang Y.P., Song C.N., Wang W.Y., Chu J.Q.;
RT "Cloning and Expression Analysis of Genes Involved in Fragrance of
RT Grapevine.";
RL Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=4-coumaroyl-CoA + 3 H(+) + 3 malonyl-CoA = 4 CO2 + 4 CoA +
CC trans-resveratrol; Xref=Rhea:RHEA:11936, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16526, ChEBI:CHEBI:45713, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57355, ChEBI:CHEBI:57384; EC=2.3.1.95;
CC Evidence={ECO:0000256|ARBA:ARBA00001104};
CC -!- PATHWAY: Phytoalexin biosynthesis; 3,4',5-trihydroxystilbene
CC biosynthesis; 3,4',5-trihydroxystilbene from trans-4-coumarate: step
CC 2/2. {ECO:0000256|ARBA:ARBA00043953}.
CC -!- SUBUNIT: Homodimer. {ECO:0000256|ARBA:ARBA00011738}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC synthases family. {ECO:0000256|ARBA:ARBA00005531,
CC ECO:0000256|RuleBase:RU003633}.
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DR EMBL; JF808008; AEP17003.1; -; mRNA.
DR AlphaFoldDB; G4XGW2; -.
DR ExpressionAtlas; G4XGW2; baseline and differential.
DR GO; GO:0050350; F:trihydroxystilbene synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0009058; P:biosynthetic process; IEA:InterPro.
DR CDD; cd00831; CHS_like; 1.
DR Gene3D; 3.40.47.10; -; 2.
DR InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR InterPro; IPR011141; Polyketide_synthase_type-III.
DR InterPro; IPR016039; Thiolase-like.
DR PANTHER; PTHR11877:SF14; CHALCONE SYNTHASE; 1.
DR PANTHER; PTHR11877; HYDROXYMETHYLGLUTARYL-COA SYNTHASE; 1.
DR Pfam; PF02797; Chal_sti_synt_C; 1.
DR Pfam; PF00195; Chal_sti_synt_N; 1.
DR PIRSF; PIRSF000451; PKS_III; 1.
DR SUPFAM; SSF53901; Thiolase-like; 2.
DR PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE 2: Evidence at transcript level;
KW Acyltransferase {ECO:0000256|RuleBase:RU003633};
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Plant defense {ECO:0000256|ARBA:ARBA00022821};
KW Stress response {ECO:0000256|ARBA:ARBA00023016};
KW Transferase {ECO:0000256|RuleBase:RU003633}.
FT DOMAIN 5..228
FT /note="Chalcone/stilbene synthase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF00195"
FT DOMAIN 238..387
FT /note="Chalcone/stilbene synthase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF02797"
FT ACT_SITE 164
FT /note="Acyl-thioester intermediate"
FT /evidence="ECO:0000256|PIRSR:PIRSR000451-1"
SQ SEQUENCE 389 AA; 42521 MW; 55BEC96AB204CE12 CRC64;
MVTVNEVRNA QRAEGPATVM AIGTATPPNC VDQSTYPDYY FRITNSEHKT ELKEKFKRMC
DKSMIKKRYM HLTEEILKEN PNVCEYMAAS LDARQDMVVV EVPKLGKEAA AKAIKEWGQP
KSKITHLVFC TTSGVDMPGA DYQLTKLLGL RPSVKRFMMY QQGCFAGGTV LRLAKDLAEN
NKGARVLVVC SEITAVTFRG PSDTHLDSLV GQALFGDGAA AVIVGSDPIP GVEKPMFELV
SAAQTILPDS DGAIDGHLRE VGLTFHLLKD VPGLISKNIE KSLNEAFQPL GIKDWNSIFW
IAHPGGPAIL DQVEEKLALK PEKLRSTRHV LSECGNMSSA CVLFILDEMR RKSAEEGLKT
TGEGPEWGVL FGFGPGLTVE TVVLHSVST
//
