ID G7IUG0_MEDTR Unreviewed; 1269 AA.
AC G7IUG0;
DT 25-JAN-2012, integrated into UniProtKB/TrEMBL.
DT 25-JAN-2012, sequence version 1.
DT 27-MAR-2024, entry version 63.
DE RecName: Full=RNA helicase {ECO:0000256|ARBA:ARBA00012552};
DE EC=3.6.4.13 {ECO:0000256|ARBA:ARBA00012552};
GN Name=11423262 {ECO:0000313|EnsemblPlants:AES67725};
GN OrderedLocusNames=MTR_2g098440 {ECO:0000313|EMBL:AES67725.1};
GN ORFNames=MtrunA17_Chr2g0329131 {ECO:0000313|EMBL:RHN76170.1};
OS Medicago truncatula (Barrel medic) (Medicago tribuloides).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; Hologalegina; IRL clade; Trifolieae; Medicago.
OX NCBI_TaxID=3880 {ECO:0000313|EMBL:AES67725.1, ECO:0000313|Proteomes:UP000002051};
RN [1] {ECO:0000313|EMBL:AES67725.1, ECO:0000313|Proteomes:UP000002051}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=A17 {ECO:0000313|EMBL:AES67725.1}, and cv. Jemalong A17
RC {ECO:0000313|EnsemblPlants:AES67725,
RC ECO:0000313|Proteomes:UP000002051};
RX PubMed=22089132; DOI=10.1038/nature10625;
RA Young N.D., Debelle F., Oldroyd G.E., Geurts R., Cannon S.B., Udvardi M.K.,
RA Benedito V.A., Mayer K.F., Gouzy J., Schoof H., Van de Peer Y., Proost S.,
RA Cook D.R., Meyers B.C., Spannagl M., Cheung F., De Mita S.,
RA Krishnakumar V., Gundlach H., Zhou S., Mudge J., Bharti A.K., Murray J.D.,
RA Naoumkina M.A., Rosen B., Silverstein K.A., Tang H., Rombauts S.,
RA Zhao P.X., Zhou P., Barbe V., Bardou P., Bechner M., Bellec A., Berger A.,
RA Berges H., Bidwell S., Bisseling T., Choisne N., Couloux A., Denny R.,
RA Deshpande S., Dai X., Doyle J.J., Dudez A.M., Farmer A.D., Fouteau S.,
RA Franken C., Gibelin C., Gish J., Goldstein S., Gonzalez A.J., Green P.J.,
RA Hallab A., Hartog M., Hua A., Humphray S.J., Jeong D.H., Jing Y.,
RA Jocker A., Kenton S.M., Kim D.J., Klee K., Lai H., Lang C., Lin S.,
RA Macmil S.L., Magdelenat G., Matthews L., McCorrison J., Monaghan E.L.,
RA Mun J.H., Najar F.Z., Nicholson C., Noirot C., O'Bleness M., Paule C.R.,
RA Poulain J., Prion F., Qin B., Qu C., Retzel E.F., Riddle C., Sallet E.,
RA Samain S., Samson N., Sanders I., Saurat O., Scarpelli C., Schiex T.,
RA Segurens B., Severin A.J., Sherrier D.J., Shi R., Sims S., Singer S.R.,
RA Sinharoy S., Sterck L., Viollet A., Wang B.B., Wang K., Wang M., Wang X.,
RA Warfsmann J., Weissenbach J., White D.D., White J.D., Wiley G.B.,
RA Wincker P., Xing Y., Yang L., Yao Z., Ying F., Zhai J., Zhou L., Zuber A.,
RA Denarie J., Dixon R.A., May G.D., Schwartz D.C., Rogers J., Quetier F.,
RA Town C.D., Roe B.A.;
RT "The Medicago genome provides insight into the evolution of rhizobial
RT symbioses.";
RL Nature 480:520-524(2011).
RN [2] {ECO:0000313|EMBL:AES67725.1, ECO:0000313|Proteomes:UP000002051}
RP GENOME REANNOTATION.
RC STRAIN=cv. Jemalong A17 {ECO:0000313|EnsemblPlants:AES67725,
RC ECO:0000313|Proteomes:UP000002051};
RX PubMed=24767513; DOI=10.1186/1471-2164-15-312;
RA Tang H., Krishnakumar V., Bidwell S., Rosen B., Chan A., Zhou S.,
RA Gentzbittel L., Childs K.L., Yandell M., Gundlach H., Mayer K.F.,
RA Schwartz D.C., Town C.D.;
RT "An improved genome release (version Mt4.0) for the model legume Medicago
RT truncatula.";
RL BMC Genomics 15:312-312(2014).
RN [3] {ECO:0000313|EnsemblPlants:AES67725}
RP IDENTIFICATION.
RC STRAIN=cv. Jemalong A17 {ECO:0000313|EnsemblPlants:AES67725};
RG EnsemblPlants;
RL Submitted (APR-2015) to UniProtKB.
RN [4] {ECO:0000313|EMBL:RHN76170.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Leaves {ECO:0000313|EMBL:RHN76170.1};
RA Pecrix Y., Gamas P., Carrere S.;
RT "Whole-genome landscape of Medicago truncatula symbiotic genes.";
RL Nat. Plants 0:0-0(2018).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC Evidence={ECO:0000256|ARBA:ARBA00001556};
CC -!- SIMILARITY: Belongs to the DEAD box helicase family. DEAH subfamily.
CC PRP16 sub-subfamily. {ECO:0000256|ARBA:ARBA00038040}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CM001218; AES67725.1; -; Genomic_DNA.
DR EMBL; PSQE01000002; RHN76170.1; -; Genomic_DNA.
DR RefSeq; XP_003597474.1; XM_003597426.2.
DR AlphaFoldDB; G7IUG0; -.
DR STRING; 3880.G7IUG0; -.
DR PaxDb; 3880-AES67725; -.
DR EnsemblPlants; AES67725; AES67725; MTR_2g098440.
DR GeneID; 11423262; -.
DR Gramene; AES67725; AES67725; MTR_2g098440.
DR KEGG; mtr:11423262; -.
DR eggNOG; KOG0924; Eukaryota.
DR HOGENOM; CLU_001832_6_2_1; -.
DR OMA; SASPWDY; -.
DR OrthoDB; 5488182at2759; -.
DR Proteomes; UP000002051; Chromosome 2.
DR Proteomes; UP000265566; Chromosome 2.
DR GO; GO:0005681; C:spliceosomal complex; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004386; F:helicase activity; IBA:GO_Central.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IBA:GO_Central.
DR CDD; cd18791; SF2_C_RHA; 1.
DR Gene3D; 1.20.120.1080; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR011709; DEAD-box_helicase_OB_fold.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR048333; HA2_WH.
DR InterPro; IPR007502; Helicase-assoc_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR18934; ATP-DEPENDENT RNA HELICASE; 1.
DR PANTHER; PTHR18934:SF91; PRE-MRNA-SPLICING FACTOR ATP-DEPENDENT RNA HELICASE PRP16; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF21010; HA2_C; 1.
DR Pfam; PF04408; HA2_N; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF07717; OB_NTP_bind; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00847; HA2; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Helicase {ECO:0000313|EMBL:AES67725.1};
KW Hydrolase {ECO:0000313|EMBL:RHN76170.1};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Reference proteome {ECO:0000313|Proteomes:UP000002051}.
FT DOMAIN 581..744
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 766..946
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
FT REGION 1..33
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 79..330
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1204..1269
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 10..24
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 101..138
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 150..239
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 285..307
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 308..330
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1204..1245
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1269 AA; 143339 MW; 13AA18D24421BC51 CRC64;
MEKNGANAEI VDINKTTTTL EPEKSTGGGL YVPGKDRVVY VAPERKSRLG LDTLAIAKRG
ESQSDGAFKV PKEITTSIAA AAEDEDKSES SDVVEESGQA GTRRNAHRRY RETTSETSRA
ESSLTDDHHA DTYGNRSTER RGSDVSASPS GYDRDDHRSE RRHSRDDSRS DSREVRHRNN
YDSRESYSGR DSRSRYYDHE YDRKRNRYEG SRRTPGRSDW DHGRWEWEDT PRRDGVSSSR
RHQPSPSPMF VGASPDARLV SPWHTPHSSY NSPSPWDHVS PSPVPIRASG SSVKSSVSGY
NRRSHKLAFS SENSDTYEEE IADKSDLGEE HKYEITESMR QEMEYDADRA WYDREEGSAL
FDSDSSSLFL GDEASFQKKE AELAKRLVRR DGTKMSLSQS KKLSQLTADN AQWEDRQLLR
SGAVRGTEVQ TEFDDEDERK VILLVHDTKP PFLDGRVVYT KQAEPIMPIK DPTSDMALIS
RKGSALVREI HEKQSSNKSR QRFWELAGSK LGDILGVEKT AEQIDADTAV VGEDGEIDFK
EEAKFSNHMK KGEAVSDFAM SKTLAEQRQY LPIFSVREEL LQVIRENQVV VVVGETGSGK
TTQLTQYLYE DGYTIGGIVG CTQPRRVAAM SVAKRVSEEM DTELGDKVGY AIRFEDVTGP
NTVIKYMTDG VLLRETLKDS DLDKYRVIVM DEAHERSLST DVLFGILKKV VAQRRDFKLI
VTSATLNAQK FSHFFGSVPI FHIPGRTFPV NILWSKTPVE DYVEGAVKQA MTIHVTSPPG
DILIFMTGQD EIEAACYSLA ERMEQMVSSS NKEVPKLLIL PIYSQLPADL QAKIFQKAED
GARKCIVATN IAETSLTVDG IFFVIDTGYG KMKVYNPRMG MDALQVFPVS RAAADQRAGR
AGRTGPGTCY RLYTESAYLN EMLASPVPEI QRTNLGNVVL LLKSLKVENL LDFDFMDPPP
QDNILNSMYQ LWVLGALNNV GGLTELGWKM VEFPLDPPLA KMLLMGERLG CLEEVLTIVS
MLSVPSVFFR PKDRAEESDA ARERFFVPES DHLTLYNVYQ QWKQHDYRGD WCNDHFLHVK
GLRKAREVRS QLLDILKTLK IPLTTCFPDT DVVRKAICSA YFHNSARLKG VGEYVNTRNG
MPCHLHPSSA LYGMGCTPDY VVYHELILTT KEYMQCATAV EPQWMAELGP MFFSVKESDT
SLLEHKKKQK REKTAMEEEM ENLKKEQAEL ERENKRKEKE KRAKSQQQIS IPGLKKGSST
FLRPKKFGL
//
