ID   G7M8X5_9CLOT            Unreviewed;       548 AA.
AC   G7M8X5;
DT   25-JAN-2012, integrated into UniProtKB/TrEMBL.
DT   25-JAN-2012, sequence version 1.
DT   27-MAR-2024, entry version 48.
DE   RecName: Full=inorganic diphosphatase {ECO:0000256|ARBA:ARBA00012146};
DE            EC=3.6.1.1 {ECO:0000256|ARBA:ARBA00012146};
DE   AltName: Full=Pyrophosphate phospho-hydrolase {ECO:0000256|ARBA:ARBA00032535};
GN   ORFNames=CDLVIII_4969 {ECO:0000313|EMBL:EHJ01460.1};
OS   Clostridium sp. DL-VIII.
OC   Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=641107 {ECO:0000313|EMBL:EHJ01460.1, ECO:0000313|Proteomes:UP000005106};
RN   [1] {ECO:0000313|EMBL:EHJ01460.1, ECO:0000313|Proteomes:UP000005106}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DL-VIII {ECO:0000313|EMBL:EHJ01460.1,
RC   ECO:0000313|Proteomes:UP000005106};
RX   PubMed=23929491;
RA   Taghavi S., Izquierdo J.A., van der Lelie D.;
RT   "Complete Genome Sequence of Clostridium sp. Strain DL-VIII, a Novel
RT   Solventogenic Clostridium Species Isolated from Anaerobic Sludge.";
RL   Genome Announc. 1:e00605-e00613(2013).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=diphosphate + H2O = H(+) + 2 phosphate; Xref=Rhea:RHEA:24576,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:43474; EC=3.6.1.1;
CC         Evidence={ECO:0000256|ARBA:ARBA00000926};
CC   -!- COFACTOR:
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC         Evidence={ECO:0000256|ARBA:ARBA00001936};
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DR   EMBL; CM001240; EHJ01460.1; -; Genomic_DNA.
DR   RefSeq; WP_009172100.1; NZ_CM001240.1.
DR   AlphaFoldDB; G7M8X5; -.
DR   STRING; 641107.CDLVIII_4969; -.
DR   eggNOG; COG1227; Bacteria.
DR   HOGENOM; CLU_025243_1_0_9; -.
DR   Proteomes; UP000005106; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR   GO; GO:0004427; F:inorganic diphosphate phosphatase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   CDD; cd04597; CBS_pair_inorgPPase; 1.
DR   Gene3D; 3.10.580.10; CBS-domain; 1.
DR   Gene3D; 3.10.310.20; DHHA2 domain; 1.
DR   Gene3D; 3.40.1390.20; HprK N-terminal domain-like; 1.
DR   Gene3D; 3.90.1640.10; inorganic pyrophosphatase (n-terminal core); 1.
DR   InterPro; IPR000644; CBS_dom.
DR   InterPro; IPR046342; CBS_dom_sf.
DR   InterPro; IPR001667; DDH_dom.
DR   InterPro; IPR038763; DHH_sf.
DR   InterPro; IPR004097; DHHA2.
DR   InterPro; IPR038222; DHHA2_dom_sf.
DR   InterPro; IPR010766; DRTGG.
DR   InterPro; IPR028979; Ser_kin/Pase_Hpr-like_N_sf.
DR   PANTHER; PTHR12112; BNIP - RELATED; 1.
DR   PANTHER; PTHR12112:SF22; MANGANESE-DEPENDENT INORGANIC PYROPHOSPHATASE; 1.
DR   Pfam; PF00571; CBS; 2.
DR   Pfam; PF01368; DHH; 2.
DR   Pfam; PF02833; DHHA2; 1.
DR   Pfam; PF07085; DRTGG; 1.
DR   SMART; SM00116; CBS; 2.
DR   SMART; SM01131; DHHA2; 1.
DR   SUPFAM; SSF54631; CBS-domain pair; 1.
DR   SUPFAM; SSF64182; DHH phosphoesterases; 1.
DR   SUPFAM; SSF75138; HprK N-terminal domain-like; 1.
DR   PROSITE; PS51371; CBS; 2.
PE   4: Predicted;
KW   CBS domain {ECO:0000256|PROSITE-ProRule:PRU00703};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000313|EMBL:EHJ01460.1};
KW   Manganese {ECO:0000256|ARBA:ARBA00023211};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW   Reference proteome {ECO:0000313|Proteomes:UP000005106}.
FT   DOMAIN          73..132
FT                   /note="CBS"
FT                   /evidence="ECO:0000259|PROSITE:PS51371"
FT   DOMAIN          252..310
FT                   /note="CBS"
FT                   /evidence="ECO:0000259|PROSITE:PS51371"
SQ   SEQUENCE   548 AA;  60444 MW;  B4C8F5EF92B67EC3 CRC64;
     MENVVYVTGH KNPDSDSICA AYGYAEFKNK TSDLPAVPVR LGNVNPETQF ILDYFGVKAP
     RLLETVKLKV EDLNFDTIKP INGNYSLRMA WNIMRDNHIK SLPVVDDKGG LIGILSISNL
     TSAYMDIGDN DILAKSKTSI ENILDTLSAT SIYINEACKV FNGKIVVSAM QPESLKEIVD
     EGDIAIVGDR PDVHEALIDI KTSLIIITGS HKLAENLLSK AKENGVTVIS TPHDSFTASR
     LLIQSIPVSY VMATKDLISF SKDELADDIK DTMAETRYNS YPVVDEEGKV IGTVSRYHLI
     SNHKKKVIQV DHNERGQSVD GLEEAEVLEI IDHHRVADIQ TSNPIFFRNE PLGSSSTIVG
     KSYFEHNITP SKEVAGLLCG AIISDTLLFK SPTCTEQDKV ICRKLAEIAG ISSLEDFAKE
     MFKAGTSLKG KSVSQIFNQD FKPFTMGESK VGVAQVNTMD IEGFMPLKDE MLSFMKSEAD
     KKGYDVVMLL LTDILTEGSQ VLVAGERQDY VEKTFNVELK DSMAFLPGVL SRKKQVIPPL
     TNVINSMK
//