ID G7Z962_AZOL4 Unreviewed; 632 AA.
AC G7Z962;
DT 25-JAN-2012, integrated into UniProtKB/TrEMBL.
DT 25-JAN-2012, sequence version 1.
DT 27-MAR-2024, entry version 73.
DE RecName: Full=Nif-specific regulatory protein {ECO:0000256|ARBA:ARBA00015308, ECO:0000256|RuleBase:RU368029};
GN Name=nifA {ECO:0000313|EMBL:CBS87522.1};
GN OrderedLocusNames=AZOLI_2308 {ECO:0000313|EMBL:CBS87522.1};
OS Azospirillum lipoferum (strain 4B).
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Rhodospirillales;
OC Azospirillaceae; Azospirillum.
OX NCBI_TaxID=862719 {ECO:0000313|EMBL:CBS87522.1, ECO:0000313|Proteomes:UP000005667};
RN [1] {ECO:0000313|Proteomes:UP000005667}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=4B {ECO:0000313|Proteomes:UP000005667};
RX PubMed=22216014; DOI=10.1371/journal.pgen.1002430;
RA Wisniewski-Dye F., Borziak K., Khalsa-Moyers G., Alexandre G.,
RA Sukharnikov L.O., Wuichet K., Hurst G.B., McDonald W.H., Robertson J.S.,
RA Barbe V., Calteau A., Rouy Z., Mangenot S., Prigent-Combaret C.,
RA Normand P., Boyer M., Siguier P., Dessaux Y., Elmerich C., Condemine G.,
RA Krishnen G., Kennedy I., Paterson A.H., Gonzalez V., Mavingui P.,
RA Zhulin I.B.;
RT "Azospirillum genomes reveal transition of bacteria from aquatic to
RT terrestrial environments.";
RL PLoS Genet. 7:E1002430-E1002430(2011).
CC -!- FUNCTION: Required for activation of most nif operons, which are
CC directly involved in nitrogen fixation. {ECO:0000256|ARBA:ARBA00002167,
CC ECO:0000256|RuleBase:RU368029}.
CC -!- SUBUNIT: Interacts with sigma-54. {ECO:0000256|ARBA:ARBA00011135,
CC ECO:0000256|RuleBase:RU368029}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; FQ311868; CBS87522.1; -; Genomic_DNA.
DR RefSeq; WP_014248509.1; NC_016622.1.
DR AlphaFoldDB; G7Z962; -.
DR STRING; 862719.AZOLI_2308; -.
DR KEGG; ali:AZOLI_2308; -.
DR HOGENOM; CLU_000445_95_2_5; -.
DR OrthoDB; 9770562at2; -.
DR Proteomes; UP000005667; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-UniRule.
DR GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-UniRule.
DR CDD; cd00009; AAA; 1.
DR Gene3D; 1.10.8.60; -; 1.
DR Gene3D; 3.30.450.40; -; 1.
DR Gene3D; 1.10.10.60; Homeodomain-like; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003018; GAF.
DR InterPro; IPR029016; GAF-like_dom_sf.
DR InterPro; IPR002197; HTH_Fis.
DR InterPro; IPR010113; Nif-specific_regulatory_prot.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR002078; Sigma_54_int.
DR InterPro; IPR025662; Sigma_54_int_dom_ATP-bd_1.
DR InterPro; IPR025943; Sigma_54_int_dom_ATP-bd_2.
DR InterPro; IPR025944; Sigma_54_int_dom_CS.
DR NCBIfam; TIGR01817; nifA; 1.
DR PANTHER; PTHR32071; TRANSCRIPTIONAL REGULATORY PROTEIN; 1.
DR PANTHER; PTHR32071:SF95; TRANSCRIPTIONAL REGULATORY PROTEIN ZRAR; 1.
DR Pfam; PF01590; GAF; 1.
DR Pfam; PF02954; HTH_8; 1.
DR Pfam; PF00158; Sigma54_activat; 1.
DR PRINTS; PR01590; HTHFIS.
DR SMART; SM00382; AAA; 1.
DR SMART; SM00065; GAF; 1.
DR SUPFAM; SSF55781; GAF domain-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS00675; SIGMA54_INTERACT_1; 1.
DR PROSITE; PS00676; SIGMA54_INTERACT_2; 1.
DR PROSITE; PS00688; SIGMA54_INTERACT_3; 1.
DR PROSITE; PS50045; SIGMA54_INTERACT_4; 1.
PE 4: Predicted;
KW Activator {ECO:0000256|ARBA:ARBA00023159, ECO:0000256|RuleBase:RU368029};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|RuleBase:RU368029};
KW Nitrogen fixation {ECO:0000256|ARBA:ARBA00023231,
KW ECO:0000256|RuleBase:RU368029};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Transcription {ECO:0000256|RuleBase:RU368029};
KW Transcription regulation {ECO:0000256|RuleBase:RU368029};
KW Two-component regulatory system {ECO:0000256|ARBA:ARBA00023012,
KW ECO:0000256|RuleBase:RU368029}.
FT DOMAIN 207..435
FT /note="Sigma-54 factor interaction"
FT /evidence="ECO:0000259|PROSITE:PS50045"
FT REGION 482..507
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 550..587
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 550..578
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 632 AA; 67872 MW; 203912B7AE312C25 CRC64;
MPSAAQSASS SSLELLTIYE VSKILGSSLD LEQTLREVLR ALSYQLQMHR GRVYLQGEDN
ALRLVAALGL PKDSTAQEID YSQGEGISGR ILKTGMPAVV PNLAEEPLFN NRSGGRDDLD
EQVASLVGVP IKAAGTVVGV LTIDRISDDG PSGHFGSDVR FLTMVANLIG QTVRLHRTVA
EERRFMMRET FRVQKELRPV VAPVNDVVCT SPNMVDVLAQ VHRVAPFKST VLIRGESGTG
KELIARAIHN LSPRKDAPFI RVNCAALPES LLESELFGHE KGSFTGAQKD HKGRFELASG
GTLFLDEIGD ISSNFQAKLL RVLQEQEFER VGGSKTIKTD VRLICATNLN LEEAVGHGKF
RADLYFRINV VTIHLPPLRE RRGDIAMLAK HFVAKFARDN GLALDIDRDA LEVLNRCTWP
GNVRELENCI ERAATQCRNG TIRVPDLSCS MNLCNSSVLF QYRTMGAAVG GLAPSMSGTP
INPSGANPAL GRPQQPPIAG MPPAVPAPAP ANGAANGVSN GTQWPACASG CSAGPAPVCG
ASKPLPPTAI APLPAPQPAA AAPAVPSAPA PQPADTPDLP LDEPESGPLR DRLVWAMERT
GWVQAKAARL LGMTTRQVSY ALRKYNIEIK RF
//
